Tropical Animal Health and Production

, Volume 47, Issue 1, pp 13–19 | Cite as

Molecular pathotyping and phylogenesis of the first Newcastle disease virus strain isolated from backyard chickens in Qatar

  • Mohamed Haroun
  • Khalid Abdelhakeem Mohran
  • Mahmoud Mahmoud Hassan
  • Nawal Mohamed Abdulla
Regular Articles

Abstract

The first Newcastle disease virus (NDV) field strain (NDV-QC08), previously isolated and identified in Qatar, was further subjected to molecular characterization and phylogenic analysis at passage levels two. Initial amplification and detection of the complementary DNA (cDNA) fragment of the NDV RNA amnioallantoic fluid (AAF) extract demonstrated the positive virus identity using real-time reverse transcriptase chain reaction (RRT-PCR). Partial sequence of the totally amplified 216 bp of the functional cleavage region encoding the F gene retrieved 112R-R-Q-K-R-F117 amino acid motif indicating the velogenic nature of the strain, and confirming the previously investigated in-vivo pathotyping. Phylogenic analysis and genotypic clustering studies classified the strain under NDV subgenotype VIId.

Keywords

Newcastle disease virus Pathotyping Genotyping Phylogenesis 

Abbreviations

ND

Newcastle disease

NDV

Newcastle disease virus

RRT-PCR

Real-time reverse transcription polymerase chain reaction

RT-PCR

Reverse transcription polymerase chain reaction

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Mohamed Haroun
    • 1
  • Khalid Abdelhakeem Mohran
    • 2
  • Mahmoud Mahmoud Hassan
    • 3
  • Nawal Mohamed Abdulla
    • 4
  1. 1.Virology Unit, Veterinary LaboratoryDepartment of Animal ResourcesDohaQatar
  2. 2.Molecular Biology Unit, Veterinary LaboratoryDepartment of Animal ResourcesDohaQatar
  3. 3.Veterinary ServicesDepartment of Animal ResourcesDohaQatar
  4. 4.Molecular Biology Unit, Veterinary LaboratoryDepartment of Animal ResourcesDohaQatar

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