Molecular detection of Cryptosporidium spp. infections in water buffaloes from northeast Thailand
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The objectives of this study were to determine the individual and herd-level prevalence and genotype of Cryptosporidium and to identify putative risk factors associated with Cryptosporidium spp. infections in water buffaloes in northeast Thailand. Fecal samples from 600 water buffaloes of 287 farms in six provinces were collected and tested using DMSO-modified acid-fast staining and polymerase chain reaction. The overall prevalence of Cryptosporidium infections in buffaloes was 5.7 and 8.7 % among individual animals and herds, respectively. The provinces with highest infected Cryptosporidium were located in the Sakon Nakhon Basin in the northern part of the region. In addition, higher herd prevalence was observed among farms with more than five buffaloes (30 %) than those with five or less animals (16.2 %). Thirty (88.2 %) of the 34 Cryptosporidium-positive samples were Cryptosporidium parvum and four (11.8 %) were Cryptosporidium ryanae.
KeywordsCryptosporidium PCR Indirect fluorescence antibody test Buffaloes Northeast Thailand
The authors wish to thank the provincial veterinary officers and water buffalo owners in the Department of Livestock Development in Ubon Ratchathani, Roi Et, Si Sa Ket, Surin, Buri Ram, and Sakon Nakhon provinces for the assistance in sample collection. This project was supported by the Kasetsart University Research Development Institute (KURDI).
Conflict of interest
The authors wish to declare that they have no conflict of interests.
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