Tropical Animal Health and Production

, Volume 46, Issue 2, pp 371–377 | Cite as

Neurological trypanosomiasis in quinapyramine sulfate-treated horses—a breach of the blood–brain barrier?

  • Muthusamy Ranjithkumar
  • Buddhi Chandrasekaran Saravanan
  • Suresh Chandra Yadav
  • Rajender Kumar
  • Rajendra Singh
  • Sahadeb Dey
Regular Articles


Trypanosoma evansi infection typically produces wasting disease, but it can also develop into a neurological or meningoencephalitis form in equids. Trypanosomiasis in horses was treated with quinapyramine sulfate, and all the 14 infected animals were recovered clinically. After clinical recovery, four animals developed a neurological form of the disease at various intervals. Two of these animals treated with diminazene aceturate recovered temporarily. Repeated attempts failed to find the parasite in the blood or the cerebrospinal fluid (CSF), but all of the animals were positive in enzyme-linked immunosorbent assay. The calculation of the antibody index (AI) in the serum and the CSF and polymerase chain reaction (PCR) analysis of the CSF and brain tissue were carried out to confirm the neuro-infection. We found PCR and AI analyses of the CSF to be useful tools in the diagnosis of the neurological form of trypanosomiasis when the organism cannot be found in the blood or CSF. The increased albumin quotient is indicative of barrier leakage due to neuroinflammation. The biochemical changes in the CSF due to nervous system trypanosomiasis include increases in the albumin quotient, total protein, and urea nitrogen. It seems to be the first report on relapse of the nervous form of trypanosomiasis in equids even after quinapyramine treatment in endemic areas.


Antibody index Diminazene aceturate Meningoencephalitis Neurological signs PCR Trypanosomiasis 


  1. Ananyutthawongese, C.T., Saengsombut, K., Sukhumsirichat, W., Uthaisang, W., Sarataphanm, N., Chansiri, K., 1999. Detection of bovine hemoparasite infection using multiplex polymerase chain reaction. Science Asia, 25, 85–90.CrossRefGoogle Scholar
  2. Berlin, D., Loeb, E., Baneth, G., 2009. Disseminated central nervous system disease caused by Trypanasoma evansi in a horse. Veterinary Parasitology, 161, 316 – 319.PubMedCrossRefGoogle Scholar
  3. Furr, M., 2002. Antigen-specific antibodies in cerebrospinal fluid after intramuscular injection of ovalbumin in horses. Journal of Veterinary Internal Medicine, 16, 588–592.PubMedCrossRefGoogle Scholar
  4. Furr, M., 2008, In: Furr, M., and Reed, S., (eds.), Equine Neurology. Blackwell publishing, Iowa, USA, pp. 47 – 218.Google Scholar
  5. Furr, M., Andrews, F., 2008. Cerebrospinal fluid and the blood – brain barrier. In: Furr, M., and Reed, S., (eds.), Equine Neurology. Blackwell Publishing, Iowa, USA, pp. 33 – 46.Google Scholar
  6. Hunter, C.A., Jennings, F.W., Adams, J.H., Murray, M., Kennedy, P.G.E., 1992. Subcurative chemotherapy and fatal post-treatment reactive encephalitis in African trypanosomiasis. The Lancet. 339(8799), 95–958.CrossRefGoogle Scholar
  7. Joshi, S.S., Singh, B., 2000. Evaluation of therapeutic and chemoprophylactic efficacy of certain drugs against clinical surra in buffalo. Indian Veterinary Journal, 77, 895–897.Google Scholar
  8. Kennedy, P.G.E., 2006. Human African trypanosomiasis—neurological aspects. Journal of Neurology, 253, 411 – 416.PubMedCrossRefGoogle Scholar
  9. Laha, R., Sasmal, N.K., 2008. Endemic status of Trypanasoma evansi infection in a horse stable of eastern region of India: a field investigation. Tropical Animal Health and Production, 40, 357 – 361.PubMedCrossRefGoogle Scholar
  10. Lanham, S.M., Godfrey, D.G., 1970. Isolation of salivarian trypanosomes from man and other animals using DEAE cellulose. Experimental Parasitology, 28, 521–534.PubMedCrossRefGoogle Scholar
  11. Lejon, V., Reiber, H., Legros, D., Dje, N., Magnus, E., Wouters, I., Sindic, C.J.M., Bu”scher, P., 2003. Intrathecal Immune response pattern for improved diagnosis of central nervous system involvement in Trypanosomiasis. Journal of Infectious Diseases, 187, 1475–1483.Google Scholar
  12. Lillie, R.D., Fulmer, H.M., 1976. Histopathologic techniques in practical histochemistry. McGraw Hill, New York.Google Scholar
  13. Masocha, W., Rottenberg, M.E., Kristensson, K., 2007. Migration of African trypanosomes across the blood–brain barrier. Physiology & Behaviour, 92, 110–114.CrossRefGoogle Scholar
  14. Monzon, C.M., Mancebo, O.A., Russo, A.M., 2003. Antibody levels by indirect ELISA test in Trypanosoma evansi infected horses following treatment with quinapyramine sulfate. Veterinary Parasitology, 111, 59–63.PubMedCrossRefGoogle Scholar
  15. Mulenga, C., Mhlanga, J.D., Kriistensson, K., Robertson, B., 2001. Trypanosoma brucei brucei crosses the blood–brain barrier while tight junction proteins are preserved in a rat chronic disease model. Neuropathology and Applied Neurobiology, 27, 77–85.PubMedCrossRefGoogle Scholar
  16. OIE - Office International Des Epizootics, 2004. Surra (Trypanasoma evansi) In: Manual of diagnostic tests and vaccines for terrestrial animals, Chapter 2.5, 15.Google Scholar
  17. Pathak, K.M.L., Arora, J.K., Kapoor, M., 1993. Camel trypanosomiasis in Rajesthan, India. Veterinary Parasitology, 49, 319–323.PubMedCrossRefGoogle Scholar
  18. Philip, K.A., Dascombe, M.J., Fraser, P.A., Pentreath, V.W., 1994. Blood–brain barrier damage in experimental African trypanosomiasis. Annals of Tropical Medicine and Parasitology, 88, 607–616.PubMedGoogle Scholar
  19. Ranjithkumar, M., Kamili, N.M., Saxena, A., Dan, Ananya, Dey, S., Raut, S.S., 2011. Disturbance of oxidant or antioxidant equilibrium in horses naturally infected with Trypanasoma evansi. Veterinary Parasitology, 180 (3–4), 349–353.PubMedCrossRefGoogle Scholar
  20. Reid, S.A., 2002. Trypanosoma evansi control and containment in Australia. Trends in Parasitology. 18 (5), 219–224.PubMedCrossRefGoogle Scholar
  21. Rodgers, J., 2010. Trypanosomiasis and the brain. Parasitology, 137, 1995–2006.PubMedCrossRefGoogle Scholar
  22. Rodrigues, A., Fighera, R.A., Souza, T.M., Sehild, A.L., Barros, C.S.L., 2009. Neuropathology of naturally occurring Trypanasoma evansi infection of horses. Veterinary Pathology, 46 (2), 251 – 258.PubMedCrossRefGoogle Scholar
  23. Seiler, R.J., Omar, S., Jackson, A.R.B., 1981. Meningoencephalitis in naturally occurring Trypanasoma evansi infection (Surra) of horses. Veterinary Pathology, 18, 120 – 122.PubMedGoogle Scholar
  24. Sellon, D.C., 2007. Surra – miscellaneous parasitic infections. In: Sellon, D.C., Long, M.T., (Eds). Equine infectious diseases. Saunders Elsevier, St.Louis MO, pp. 475 – 476.Google Scholar
  25. Terlizzi, R.D., Platt, S.R. 2009. The function, composition and analysis of cerebrospinal fluid in companion animals, Part –II – Analysis. Veterinary Journal, 80 (1), 15 – 32.CrossRefGoogle Scholar
  26. Tuntasuvan, D., Sarataphan, N., Nishikawa, H., 1997. Cerebral trypanasomiasis in native cattle. Veterinary Parasitology, 73, 357 – 363.PubMedCrossRefGoogle Scholar
  27. Tuntasuvan, D., Mimapan, S., Sarataphanm, N., Trongwongsa, L., Intraraksa, R., Lukins, A.G., 2000. Detection of Trypansoma evansi in brains of the naturally infected hog deer by streptavidine – biotin immunohistochmistry. Veterinary Parasitology, 87, 223 – 230.PubMedCrossRefGoogle Scholar
  28. Whitelaw, D.D., Gardiner, P.R., Murray, M., 1988. Extravasular foci of Trypansoma vivax in goats: the central nervous system and aqueous humour of the eye as potential sources of relapse infections after chemotherapy. Parasitology, 97, 51–61.PubMedCrossRefGoogle Scholar
  29. WHO (1998) Control and surveillance of African trypanasomiasis. Report of a WHO expert committee. World health organization. Geneva, Switzerland, Technical report series No. 881, pp. 114.Google Scholar
  30. Yadav, S.C., Kumar, R., Kumar, S., Tatu, U., Singh, R.K., Gupta, A.K., 2011. Identification and characterization of cysteine proteinases of Trypanosoma evansi. Parasitology Research, 109, 559–565.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Muthusamy Ranjithkumar
    • 1
  • Buddhi Chandrasekaran Saravanan
    • 2
  • Suresh Chandra Yadav
    • 3
  • Rajender Kumar
    • 3
  • Rajendra Singh
    • 4
  • Sahadeb Dey
    • 1
  1. 1.Division of MedicineIndian Veterinary Research InstituteIzatnagarIndia
  2. 2.Division of ParasitologyIndian Veterinary Research InstituteIzatnagarIndia
  3. 3.National Research Centre on EquineHisarIndia
  4. 4.Division of PathologyIndian Veterinary Research InstituteIzatnagarIndia

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