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The diversity of BVDV subgenotypes in a vaccinated dairy cattle herd in Brazil

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Bovine viral diarrhoea virus (BVDV) is an important pathogen of cattle that occurs worldwide with substantial economic impact on beef and dairy industries. The aim of this study was to describe the diversity of BVDV subgenotypes in persistently infected (PI) animals identified in a highly productive, regularly vaccinated, dairy cattle herd presenting with reproductive failure. Serum samples were collected from all animals within the herd (n = 692) and used to detect the presence of BVDV RNA. Using reverse transcription polymerase chain reaction assay, 29 cows were identified as transiently infected, three animals (two cows and one calf) as persistently infected, and one calf as putative BVDV PI animal. The sequences of 5'UTR and/or Npro gene of BVDV used in phylogenetic analyses revealed that the three PI animals were infected by three different BVDV subgenotypes (BVDV-1a, BVDV-1b, and BVDV-1d). These results demonstrated that in an open dairy cattle herd, regular vaccination against BVDV by itself is not able to prevent viral circulation in the herd. Furthermore, depending on the frequency of the acquisition of heifers and/or cows for replacement, several BVDV subgenotypes may co-exist simultaneously in the same herd.

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  1. Becher, P., Avalos Ramirez, R., Orlich, M., Cedillo Rosales, S., König, M., Schweizer, M., Stalder, H., Schirrmeier, H. and Thiel, H.J., 2003. Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology, 311, 96--104

  2. Bolin, S.R., Lim, A., Grotelueschen, D.M., Mcbeth, W.W. and Cortese, V.S., 2009. Genetic characterization of bovine viral diarrhea viruses isolated from persistently infected calves born to dams vaccinated against bovine viral diarrhea virus before breeding. American Journal of Veterinary Research, 70, 86--91

  3. Boom, R., Sol, C.J.A., Salimans, M.M.M., Jansen, C.L., Wertheimdillen, P.M.E. and Noordaa, J., 1990. Rapid and simple method for purification of nucleics acids. Journal of Clinical Microbiology, 28, 495--503

  4. Brock, K.V., 2003. The persistence of bovine viral diarrhea virus. Biologicals, 31, 133--135

  5. Cortese, V.S., Grooms, D.L., Ellis, J., Bolin, S.R., Ridpath, J.F. and Brock, K.V., 1998. Protection of pregnant cattle and their fetuses against infection with bovine viral diarrhea virus type 1 by use of a modified-live virus vaccine. American Journal of Veterinary Research, 59, 1409--1413

  6. Cortez, A., Heinemann, M.B., Castro, A.M.M.G., Soares, R.M., Pinto, A.M.V., Alfieri, A.A., Flores, E.F., Leite, R.C. and Richtzenhain, L.J., 2006. Genetic characterization of Brazilian bovine viral diarrhea virus isolates by partial nucleotide sequencing of the 5’-UTR region. Pesquisa Veterinária Brasileira, 26, 211--216

  7. Decaro, N., Lucente, M.S., Mari, V., Cirone, F., Cordioli, P., Camero, M., Sciarretta, R., Losurdo, M., Lorusso, E. and Buonavoglia, C., 2011. Atypical pestivirus and severe respiratory disease in calves, Europe. Emerging Infectious Diseases, 17, 1549--1552

  8. Dias, F.C., Médici, K.C., Alexandrino, B., Medeiros, A.S.R., Alfieri, A.A. and Samara, S.I., 2010. Occurrence of persistently infected animals with bovine viral diarrhoea virus in cattle herds from the States of Minas Gerais and São Paulo, Brazil. Pesquisa Veterinária Brasileira, 30, 933--939

  9. Ficken, M.D., Ellsworth, M.A. and Tucker, C.M., 2006. Evaluation of the efficacy of a modified-live combination vaccine against bovine viral diarrhea virus types 1 and 2 challenge exposures in a one-year duration-of-immunity fetal protection study. Veterinary Therapeutics: Research in Applied Veterinary Medicine, 7, 283--294

  10. Flores, E.F., Ridpath, J.F., Weiblen, R., Vogel, F.S. and Gil, L.H., 2002. Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: Evidence for a subgenotype within BVDV-2. Virus Research, 87, 51--60

  11. Fulton, R.W., Ridpath, J.F., Confer, A.W., Saliki, J.T., Burge, J.L. and Payton, M.E., 2003. Bovine viral diarrhoea virus antigenic diversity: Impact on disease and vaccination programs. Biologicals, 31, 89--95

  12. Fulton, R.W., Ridpath, J.F., Ore, S., Confer, A.W., Saliki, J.T., Burge, L.J. and Payton, M.E., 2005. Bovine viral diarrhea virus (BVDV) subgenotypes in diagnostic laboratory accessions: Distribution of BVDV1a, 1b, and 2a subgenotypes. Veterinary Microbiology, 111, 135--140

  13. Fulton, R.W., Johnson, B.J., Briggs, R.E., Ridpath, J.F., Saliki, J.T., Confer, A.W., Burge, L.J., Step, D.L., Walker, D.A. and Payton, M.E., 2006. Challenge with bovine viral diarrhea virus by exposure to persistently infected calves: Protection by vaccination and negative results of antigen testing in non vaccinated acutely infected calves. Canadian Journal of Veterinary Research, 70, 121--127

  14. Houe, H., Baker, J.C., Maes, R.K., Wuryastuti, H., Wasito, R., Ruegg, P.L., Lloyd, J.W., 1995. Prevalence of cattle persistently infected with bovine viral diarrhea virus in 20 dairy herds in two counties in central Michigan and comparison of prevalence of antibody-positive cattle among herds with different infection and vaccination status. Journal of Veterinary Diagnostic Investigation, 7, 321--326

  15. Houe, H., 1999. Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV). Veterinary Microbiology, 64, 89--107

  16. ICVT, 2012. International Committee on Taxonomy of Viruses <http://ictvonline.org/virusTaxonomy.asp?taxnode_id=20123618>Accessed in: 25 mar. 2013.

  17. Kovács, F., Magyar, T., Rinehart, C., Elbers, K., Schlesinger, K. and Ohnesorge, W.C., 2003. The live attenuated bovine viral diarrhea virus components of a multi-valent vaccine confer protection against fetal infection. Veterinary Microbiology, 96, 117--131

  18. Lindeberg, A. and Houe, H., 2005. Characteristics in the epidemiology of bovine viral diarrhea virus (BVDV) of relevance control. Preventive Veterinary Medicine, 72, 55--73

  19. Lunardi, M., Headley, S.A., Lisbôa, J.A., Amude, A.M. and Alfieri, A.A., 2008. Outbreak of acute bovine viral diarrhea in Brazilian beef cattle: Clinicopathological findings and molecular characterization of a wild-type BVDV strain subtype 1b. Research in Veterinary Science, 85, 599--604

  20. Mahony, T.J., Mccarthy, F.M., Gravel, J.L., Corney, B., Young, P.L. and Vilcek, S., 2005. Genetic analysis of bovine viral diarrhoea viruses from Australia. Veterinary Microbiology, 106, 1--6

  21. Oliveira, L.G., Oliveira, E.A.S. and Silva, L.H.T., 1996. Presence of pestiviruses and antibodies to pestiviruses in sera and cell cultures. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 48, 513--521

  22. Peterhans, E., Jungi, T.W. and Schweizer, M., 2003. BVDV and innate immunity. Biologicals, 31, 107--112

  23. Peterhans, E., Bachofen, C., Stalder, H. and Schweizer, M., 2010. Cytopathic bovine viral diarrhea viruses (BVDV): emerging pestiviruses doomed to extinction. Veterinary Research, 41, 44--58

  24. Ridpath, J.F., 2003. BVDV genotypes and biotypes: practical implications for diagnosis and control. Biologicals, 31, 127--131

  25. Ridpath, J.F., 2005. Classification and molecular biology. In: Goyal, S.M. and Ridpath, J.F. (eds), Bovine viral diarrhea virus: diagnosis, management and control. 1st Edition. (Iowa: Blackwell Publishing), 65--80

  26. Ridpath, J.F., 2010. Bovine viral diarrhea virus: global status. The Veterinary Clinics of North America. Food Animal Practice, 26, 105--121

  27. Stahl, K., Benito, A., Felmer, R., Zuñiga, J., Reinhardt, G., Rivera, H., Baule, C. and Moreno-López, J., 2009. Genetic diversity of bovine viral diarrhoea virus (BVDV) from Peru and Chile. Pesquisa Veterinária Brasileira, 29, 41--44

  28. Toplak, I., Sandvik, T., Barlic-Maganja, D., Grom, J. and Paton, D.J., 2004. Genetic typing of bovine viral diarrhoea virus: Most Slovenian isolates are of genotypes 1d and 1 f. Veterinary Microbiology, 99, 175--185

  29. Uttenthal, A., Stadejek, T. and Nylin, B., 2005. Genetic diversity of bovine viral diarrhoea viruses (BVDV) in Denmark during a 10-year eradication period. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 113, 536--541

  30. Vilcek, S., Paton, D.J., Durkovic, B., Strojny, L., Ibata, G., Moussa, A., Loitsch, A., Rossmanith, W., Vega, S., Scicluna, M.T. and Paifi, V., 2001. Bovine viral diarrhoea virus genotype 1 can be separated into at least eleven genetic groups. Archives of Virology, 146, 99--115

  31. Vilcek, S., Durkovic, B., Kolesarova, M., Greiser-Wilke, I. and Paton, D., 2004. Genetic diversity of international bovine viral diarrhoea virus (BVDV) Isolates: Identification of a new BVDV-1 genetic group. Veterinary Research, 35, 609--615

  32. Weinstock, D., Bhudevi, B., Anthony, E.C., 2001 Single-tube single-reverse transcriptase pcr assay for detection of bovine viral diarrhea virus in pooled bovine serum. Journal of Clinical Microbiology, 39, 343--346

  33. Zimmer, G.M., Wentink, G.H., Bruschke, C., Westenbrink, F.J., Brinkhof, J. and De Goey, I., 2002. Failure of foetal protection after vaccination against an experimental infection with bovine virus diarrhea virus. Veterinary Microbiology, 89, 255--265

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The authors thank the Brazilian Institutes CNPq, CAPES, FINEP, and Araucaria Foundation for financial support. A. F. Alfieri, S. A. Headley, and A. A. Alfieri are recipients of CNPq fellowships.

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Correspondence to Amauri A. Alfieri.

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Otonel, R.A.A., Alfieri, A.F., Dezen, S. et al. The diversity of BVDV subgenotypes in a vaccinated dairy cattle herd in Brazil. Trop Anim Health Prod 46, 87–92 (2014). https://doi.org/10.1007/s11250-013-0451-y

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  • Bovine
  • Persistent infection
  • Bovine viral diarrhoea
  • Genotyping
  • 5'UTR
  • Npro