Tropical Animal Health and Production

, Volume 46, Issue 1, pp 73–78 | Cite as

Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR

  • Shahzad Ali
  • Qurban Ali
  • Falk Melzer
  • Iahtasham Khan
  • Shamim Akhter
  • Heinrich Neubauer
  • Syed M. Jamal
Regular Articles

Abstract

Brucellosis is endemic in bovines in Pakistan. The Brucella species and biovars involved, however, are unknown. The objectives of the present study were to isolate and characterize brucellae from seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes which had recently aborted. The seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes were collected from the Potohar Plateau, Pakistan. Isolation of brucellae was done on modified Farrell’s serum dextrose agar. Isolates were characterized by conventional biotyping methods, while molecular typing was done by genus (B4/B5) and species-specific (Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis) polymerase chain reaction (PCR). A total of 30 isolates were recovered from milk (n = 5), aborted fetuses (n = 13), and vaginal swabs (n = 12). Most isolates were from cattle (56.7 %). All of them were identified as B. abortus biovar 1 based on conventional biotyping methods and genus and species-specific PCR. This preliminary study provides the first report on the prevalence of B. abortus biovar 1 in cattle and buffaloes in Pakistan.

Keywords

Brucella abortus Pakistan Biochemical tests PCR 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Shahzad Ali
    • 1
    • 2
    • 3
  • Qurban Ali
    • 3
  • Falk Melzer
    • 2
  • Iahtasham Khan
    • 2
    • 4
  • Shamim Akhter
    • 1
  • Heinrich Neubauer
    • 2
  • Syed M. Jamal
    • 3
    • 5
  1. 1.Pir Mehr Ali Shah Arid Agriculture UniversityRawalpindiPakistan
  2. 2.Friedrich-Loeffler-InstitutBundesforschungsinstitut für TiergesundheitJenaGermany
  3. 3.National Veterinary LaboratoryIslamabadPakistan
  4. 4.University of Veterinary and Animal SciencesLahorePakistan
  5. 5.Ministry of National Food Security and ResearchIslamabadPakistan

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