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Tropical Animal Health and Production

, Volume 45, Issue 7, pp 1557–1561 | Cite as

Survey of Anaplasmataceae bacteria in sheep from Senegal

  • Mamadou Lamine Djiba
  • Oleg Mediannikov
  • Mbaye Mbengue
  • Yaya Thiongane
  • Jean-François Molez
  • Momar Talla Seck
  • Florence Fenollar
  • Didier Raoult
  • Mady Ndiaye
Regular Articles

Abstract

Purpose

The authors studied the role of bacteria belonging to Anaplasmataceae family as the causes of acute illnesses of sheep in West Africa.

Methods

We examined and sampled 120 febrile sheep in two regions of Senegal for this study. The DNA extracted from these blood samples was tested by PCR using two pairs of primers (groEL-based and 16S rRNA gene-based).

Results

In 52/120 samples, the microscopic examination revealed intraerythrocytic and/or intraphagocytic spherical inclusions. In 48/52 cases, we succeeded in identifying the bacterial agent: in 38 cases, it was Anaplasma ovis; in six cases, it was Ehrlichia ruminantium; in two cases, Anaplasma phagocytophilum; in one case, Anaplasma platys; and in one case, a yet uncultured Anaplasma sp. closely related to A. phagocytophilum.

Conclusions

Our studies demonstrated the great variety of pathogenic bacteria from the Anaplasmataceae family in the blood of clinically ill sheep. A. ovis was identified unexpectedly often. For the first time, A. phagocytophilum was found in sub-Saharan Africa, and its further epidemiology may be now reconsidered. The roles of canine pathogen, A. platys, and yet undescribed Anaplasma sp. “Badiouré” in ovine pathology should be more closely studied.

Keyword

Sheep Anaplasmosis Ehrlichiosis Ixodid ticks Senegal 

Notes

Acknowledgments

The present work was partly supported by an Agence National de Recherche (ANR) grant 2010 MALEMAF (research on emergent pathogens in Africa) and the Foundation Mediterranée Infection. We thank the villagers of Niayes and Ziguinchor regions for their benevolence.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Mamadou Lamine Djiba
    • 1
  • Oleg Mediannikov
    • 2
  • Mbaye Mbengue
    • 1
  • Yaya Thiongane
    • 1
  • Jean-François Molez
    • 1
  • Momar Talla Seck
    • 1
  • Florence Fenollar
    • 2
  • Didier Raoult
    • 2
  • Mady Ndiaye
    • 3
  1. 1.Department of MicrobiologyNational Laboratory for Research on Animal DiseasesDakarSenegal
  2. 2.URMITE, IRD 198, UM63, CNRS 7278, Inserm 1095Aix Marseille UniversitéDakarSenegal
  3. 3.Department for Animal Biology, Faculty for Sciences and TechniquesDakar University (UCAD)DakarSenegal

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