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Detection and differentiation of pigeon paramyxovirus serotype-1 (PPMV-1) isolates by RT-PCR and restriction enzyme analysis

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Abstract

Detection and pathotyping of Newcastle disease virus (NDV) is extremely important because the appearance of virulent virus has significant economic consequences. During 1981 to 1985, infections of racing and show pigeons with an avian paramyxovirus serotype-1 (APMV-1) hit worldwide, and a panzootic occurred due to a variant form of classical NDV. On the basis of pathogenicity and monoclonal antibody binding studies, the virus was termed ‘pigeon PMV-1’ (PPMV-1). In the past, number of Newcastle disease outbreaks in poultry and other birds has been attributed to PPMV-1. PPMV-1 viruses are known to present difficulty when assessed by conventional in vivo pathogenicity tests. In this study, the technique of reverse transcription-polymerase chain reaction (RT-PCR) and restriction enzyme (RE) analysis was used to detect and differentiate PPMV-1 isolates of Indian origin. Restriction enzyme digestion analysis of RT-PCR-amplified fusion protein (F) gene, encoding for the cleavage activation sites of fusion protein, was carried out with restriction enzymes BglI, HhaI, HaeIII, HinfI, MboI, MspI, PvuII and StyI. A set of only four enzymes HhaI, MspI or HaeIII, MboI and BglI alone were sufficient to differentially detect APMV-1 and PPMV-1 viruses and their pathotypes. In conclusion, RT-PCR followed by RE analysis proved to be useful for detection and differentiation of APMV-1 and PPMV-1 isolates at genomic level.

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References

  • Alexander, D.J., 1998. Newcastle disease and other avian paramyxoviruses. In: D.E., Swayne, J.R., Glison, M.W. Jackwood, J.E., Pearson, W. M. Reed (eds), A laboratory manual for the isolation and identification of avian pathogens, 4th edn. American Association of Avian Pathologists, Pennsylvania, pp. 156–163.

  • Alexander, D.J. and Parsons, G., 1984. Avian paramyxovirus type 1 infections of racing pigeon: 2. Pathogenicity experiments in pigeons and chickens. Veterinary Record, 114, 466–468.

    Article  PubMed  CAS  Google Scholar 

  • Alexander, D.J. and Parsons, G., 1986. Pathogenicity for chickens of avian paramyxovirus type 1 isolates obtained from pigeon in Great Britain during 1983–85. Avian Pathology, 15, 487–493.

    Article  PubMed  CAS  Google Scholar 

  • Alexander, D.J. and Senne, D.A., 2008. Newcastle disease, other avian paramyxoviruses, and pneumovirus infections. In: Saif, Y.M., A.M. Fadly, J.R. Glisson, L.R. McDougald, L.K. Nolan, D. E. Swayne (eds), Disease of poultry, 12th edn. Blackwell, Ames, pp. 75–115.

    Google Scholar 

  • Alexander, D.J., G. Parsons, and R. Marshall, 1984a: Infection of fowls with Newcastle disease virus by food contaminated with pigeon feces. Vet Rec. 115:601–602.

    Article  PubMed  CAS  Google Scholar 

  • Alexander, D.J., Russel, P.H. and Collins, M.S., 1984b. Paramyxovirus type 1 infections of racing pigeons: 1. Characterization of isolated viruses. Veterinary Record, 114, 444–446.

    Article  PubMed  CAS  Google Scholar 

  • Alexander, D.J., Russel, P.H., Parsons, G., Abu Elzein, E.M.E., Ballough, A., Cernik, K., Engstrom, B., Fevereiro, M., Fleury, H.J.A., Guitet, M., Kaleta, E.F., Kihm, U., Kosters, J., Lomniczi, J. Meister, B., Meulemans, G., Nerome, K., Petek, M., Pokomunski, S., Polten, B., Prip, M., Richter, R., Saghy, E., Samberg, V., Spanoghe, L. and Tumova, B., 1985. Antigenic and biological characterization of avian paramyxovirus type-1 isolates from pigeons—an international collaborative study. Avian Pathology, 14, 365–376.

    Article  PubMed  CAS  Google Scholar 

  • Alexander, D.J. E.W. Aldous and M.C.M. Fuller, 2012: The long view: a selective review of 40 years of Newcastle disease research. Avian Pathology, 41, 329–335.

    Article  PubMed  Google Scholar 

  • Bonfante, F., Terregino, C., Heidari, A., Monne, I., Salviato, A., Taddei, R., Raffini, E. and Capua, I., 2012. Identification of APMV-1 associated with high mortality of collared doves (Streptoelia decaocto) in Italy. Veterinary Record, 171, 327.

    Article  PubMed  CAS  Google Scholar 

  • Cattoli, G., Susta, L., Terregino, C. and Brown, C., 2011. Newcastle disease: a review of field recognition and current methods of laboratory detection. Journal of Veterinary Diagnostic Investigation, 23, 637–656.

    Google Scholar 

  • Collins, M.S., Strong, I. and Alexander, D.J. 1996. Pathogenicity and phylogenetic evaluation of the variant Newcastle disease virus termed ‘pigeon PMV-1 viruses’ based on the nucleotide sequence of the fusion protein gene. Archives of Virology, 141, 635–647.

    Article  PubMed  CAS  Google Scholar 

  • Dortmans, J.C., Koch, G., Rottier P.J.M. and Peeters, B.P.H., 2009. Virulence of pigeon paramyxovirus type 1 does not always correlate with the cleavability of its fusion protein. Journal of General Virology, 90, 2746–2750.

    Article  PubMed  CAS  Google Scholar 

  • Dortmans J.C., Koch, G., Rottier, P.J. and Peeters, B.P., 2011. A comparative infection study of pigeon and avian paramyxovirus type 1 viruses in pigeons: evaluation of clinical signs, virus shedding and seroconversion. Avian Pathology, 40, 125–130.

    Article  PubMed  CAS  Google Scholar 

  • Hines, L. and Miller, C.L., 2012. Avian Paramyxovirus Serotype-1: A Review of Disease Distribution, Clinical Symptoms, and Laboratory Diagnostics. Veterinary Medicine International. doi:10.1155/2012/708216.

  • ICTV (2011). Virus Taxonomy: 2011 Release. http://ictvonline.org/virusTaxonomy.asp?version=2011. Accessed 5 July 2012.

  • Irvine, R.M., Aldous, E.W., Manvell, R.J., Cox, W.J., Ceeraz, V., Fuller, C.M., Wood, A.M., Milne, J.C., Wilson, M., Hepple, R.G., Hurst, A., Sharpe, C.E., Alexander, D.J. and Brown, I.H., 2009. Outbreak of Newcastle disease due to pigeon paramyxovirus type 1 in grey partridges (Perdix perdix) in Scotland in October 2006. Veterinary Record, 165, 531–535.

    Article  PubMed  CAS  Google Scholar 

  • Kant, A., Koch, G., Van Roozelaar, D., Balk, F. and Ter Huurne, A. 1997. Differentiation of virulent and non-virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction. Avian Pathology, 26, 837–849.

    Article  PubMed  CAS  Google Scholar 

  • Kataria, J.M., Dhama, K., Rahul, S., Singh S.D. and Sah, R.L., 2004. Occurrence of Newcastle disease in pigeons in India and immune response following immunization with inactivated homologous and heterologous paramyxovirus. Indian Journal of Comparative Microbiology Immunology and Infectious Diseases, 25, 53–56.

    Google Scholar 

  • Kim, L.M., King, D.J., Guzman, H., Tesh, R.B., Travassos da Rosa A.P., Bueno, R. Jr,, Dennett, J.A., Afonso, C.L., 2008. Biological and phylogenetic characterization of pigeon paramyxovirus serotype 1 circulating in wild North American pigeons and doves. Journal of Clinical Microbiology,46:3303–3310.

    Article  PubMed  CAS  Google Scholar 

  • Kommers, G.D., King, D.J., Seal, B.S. and Brown, C.C., 2001. Virulence of Pigeon-origin Newcastle disease virus isolates for domestic chickens. Avian Diseases, 45, 906–921.

    Article  PubMed  CAS  Google Scholar 

  • Liu, H., Wang, Z., Son, C., Wang, Y., Yu, B., Zheng, D., Sun, C. and Wu, Y., 2006. Characterization of pigeon-origin Newcastle disease virus isolated in China. Avian Diseases, 50, 636–640.

    Article  PubMed  Google Scholar 

  • Nanthakumar, T., Kataria, R.S., Tiwari, A.K., Butchaiah, G. and Kataria, J.M., 2000. Pathotyping of Newcastle disease viruses by RT-PCR and restriction enzyme analysis. Veterinary Research Communication, 24, 275–286.

    Article  CAS  Google Scholar 

  • Naveen, K.A., Singh, S.D. and Mohanty, T.R., 2009. Pathobiological and molecular characterization of an Indian isolate of Pigeon paramyxovirus type-I. Indian Journal of Veterinary Pathology, 33, 58-61.

    Google Scholar 

  • OIE, 2012. Newcastle disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012. Chapter 2.3.14. http://www.oie.int/international-standard-setting/terrestrial-manual/access-online/ Accessed 5 July 2012

  • Tirumurugaan, K.G., Kapgate, S., Vinupriya, M.K., Vijayarani, K., Kumanan, K., Elankumaran, S., 2011. Genotypic and pathotypic characterization of Newcastle disease viruses from India. PLoS One, 6:e28414.

    Article  PubMed  CAS  Google Scholar 

  • Ujvári, D., Wehmann, E., Herczeg, J. and Lomniczi, B., 2006. Identification and subgrouping of pigeon type Newcastle disease virus strains by restriction enzyme cleavage site analysis. Journal of Virological Methods, 131, 115–121.

    Article  PubMed  Google Scholar 

  • Wakamatsu, N., King, D.J., Kapczynski, D.R., Seal, B.S., and Brown, C.C., 2006. Experimental Pathogenesis for Chickens, Turkeys, and Pigeons of Exotic Newcastle Disease Virus from an Outbreak in California during 2002–2003.Veterinary Pathology, 43:925–933.

    Google Scholar 

  • Zanetti, F., Rodríguez, M., King, D.J., Capua, I., Carrillo, E., Seal, B.S., Berinstein, A., 2003. Matrix protein gene sequence analysis of avian paramyxovirus 1 isolates obtained from pigeons. Virus Genes. 26, 199–206.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors are thankful to the Indian Council of Agricultural Research, New Delhi for providing necessary grants and facilities to carry out this research work.

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Authors and Affiliations

  1. Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India

    Kuttanda A. Naveen, Shambhu Dayal Singh, Jag Mohan Kataria, Rajamani Barathidasan & Kuldeep Dhama

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  1. Kuttanda A. Naveen
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  2. Shambhu Dayal Singh
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  3. Jag Mohan Kataria
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  5. Kuldeep Dhama
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Correspondence to Kuldeep Dhama.

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Naveen, K.A., Singh, S.D., Kataria, J.M. et al. Detection and differentiation of pigeon paramyxovirus serotype-1 (PPMV-1) isolates by RT-PCR and restriction enzyme analysis. Trop Anim Health Prod 45, 1231–1236 (2013). https://doi.org/10.1007/s11250-013-0352-0

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