Tropical Animal Health and Production

, Volume 44, Supplement 1, pp 25–31 | Cite as

Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

  • Erastus K. Kang’ethe
  • Erastus K. Mulinge
  • Robert A. Skilton
  • Moses Njahira
  • Joseph G. Monda
  • Concepta Nyongesa
  • Cecilia K. Mbae
  • Stanley K. Kamwati
SI (Emerging Zoonoses)

Abstract

A total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twenty-five samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.

Keywords

Cryptosporidium Genotyping Dairy farming Kenya 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Erastus K. Kang’ethe
    • 1
  • Erastus K. Mulinge
    • 2
  • Robert A. Skilton
    • 3
  • Moses Njahira
    • 3
  • Joseph G. Monda
    • 1
  • Concepta Nyongesa
    • 1
  • Cecilia K. Mbae
    • 2
  • Stanley K. Kamwati
    • 2
  1. 1.Department of Public Health, Pharmacology and ToxicologyUniversity of NairobiNairobiKenya
  2. 2.Centre for Microbiology ResearchKenya Medical Research InstituteNairobiKenya
  3. 3.Bioscience eastern and central Africa-International Livestock Research Institute Hub (BecA-ILRI Hub)NairobiKenya

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