RT-PCR test for detecting porcine sapovirus in weanling piglets in Hunan Province, China
- 176 Downloads
The prevalence of porcine sapovirus infection in weanling pigs was investigated in Hunan Province, China, between August 2006 and October 2007. A total of 153 diarrheic fecal samples from ten intensive pig farms from ten representative administrative regions in Hunan province were examined for porcine sapoviruses using RT-PCR. Twenty-two of 153 (14.37 %) samples were found to contain porcine sapoviruses. Phylogenetic analysis showed that all the porcine sapovirus isolates in Hunan Province belonged to the porcine sapovirus genogroup III. The results of the present investigation have implications for the control of porcine sapovirus infection in pigs in Hunan Province, China.
KeywordsPorcine sapovirus Prevalence Swine RT-PCR Phylogenetic analysis Hunan province China
This work was supported by the National Natural Science Foundation of China (project no. 30571390) and the Science and Technology Foundation of Hunan Province (grant no. 2007FJ1003).
- Flynn, W.T., Saif, L.J., Moorhead, P.D., 1988. Pathogenesis of porcine enteric calicivirus-like virus in four-day-old gnotobiotic pigs. American Journal of Veterinary Research, 1988, 49, 819–825.Google Scholar
- Guo, M., Hayes, J., Cho, K.O., Parwani, A.V., Lucas, L.M., Saif, L.J., 2001. Comparative pathogenesis of tissue culture-adapted and wild-type Cowden porcine enteric calicivirus (PEC) in gnotobiotic pigs and induction of diarrhea by intravenous inoculation of wild-type PEC. Journay of Virology, 75: 9239–9251.PubMedCrossRefGoogle Scholar
- Jin, M., Yu, J.M., Li, H.Y., Zang, Q. and Cui, S.X., 2010. Genetic Diversity of Porcine Sapoviruses from Lulong County in China. Chinese Journal Virology, 26, 255–259 (in Chinese).Google Scholar
- Martella, V., Bányai, K., Lorusso, E., Bellacicco, A.L., Decaro, N., Mari, V., Saif, L., Costantini, V., De Grazia, S., Pezzotti, G., Lavazza, A., Buonavoglia, C. 2008. Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets. Virus Genes, 36: 365–373.PubMedCrossRefGoogle Scholar
- Nakamura, K., Saga, Y., Iwai, M., Obara, M., Horimoto, E., Hasegawa, S., Kurata, T., Okumura, H., Nagoshi, M. and Takizawa, T., 2010. Frequent detection of noroviruses and sapoviruses in swine and high genetic diversity of porcine sapovirus in Japan during Fiscal Year 2008. Jouranl of Clinical Microbiology, 48, 1215–1222.PubMedCrossRefGoogle Scholar
- Page, R.D., 1996. TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Application Bioscience, 12, 357–358.Google Scholar
- Swofford, D.L., 2002. Paup*: Phylogenetic Analysis Using Parsimony, version 4.0b10. Sinauer Associates, Sunderland, MA.Google Scholar