Advertisement

Tropical Animal Health and Production

, Volume 44, Issue 7, pp 1497–1503 | Cite as

Detection of Pasteurella multocida isolates from local pigs of India by polymerase chain reaction and their antibiogram

  • Swaraj RajkhowaEmail author
  • Ingudam Shakuntala
  • Seema Rani Pegu
  • Rajib Kumar Das
  • Anubrata Das
Original Research

Abstract

Pasteurella multocida has been recognized as an important veterinary pathogen for over a century. Conventional methods for diagnosis of pasteurellosis rely on the detection of the organism by microscopy and its isolation and identification. However, as far as pasteurellosis is concerned, it is not just sufficient to know the identity of the organism. To constitute effective control measures, it is important to know the serotype of the organism. A study was undertaken to characterize the Pasteurella isolates from local pigs in India with clinical respiratory disease by determination of their capsule types and presence or absence of toxin gene. Pasteurella could be isolated from 66.70% of pigs with clinical respiratory disease. All the isolates were confirmed through biochemical characterization and P. multocida-specific polymerase chain reaction. It has also been observed that all the isolates belonged to capsular type D. All the isolates were sensitive to chloramphenicol, chlortetracycline, doxycycline, and enrofloxacin, while the rest of the antibiotics were less effective. It has also been observed that all isolates were resistant to cephalexin, penicillin G, and sulphadiazine. The study revealed the detection of P. multocida serotype D from clinical respiratory diseases of local pigs of India, which could be one of the important respiratory tract pathogens responsible for mortality of local pigs in India.

Keywords

Antibiogram Clinical respiratory tract disease Local pigs Pasteurella multocida Polymerase chain reaction (PCR) 

References

  1. Arumugam, N.D., Ajam, N., Blackall, P.J., Asiah, N.M., Ramlan, M., Maria, J., Yuslan, S. and Thong, K.L., 2011. Capsular serotyping of Pasteurella multocida from various animal hosts—a comparison of phenotypic and genotypic methods, Tropical Biomedicine, 28,55-63.PubMedGoogle Scholar
  2. Boyce, J.D., Chung, J.Y. and Adler, B., 2000. Pasteurella multocida capsule: composition, function and genetics, Journal of Biotechnology, 83, 153-160.PubMedCrossRefGoogle Scholar
  3. Carter, G.R., 1967. Pasteurellosis: Pasteurella multocida and Pasteurella hemolítica, Advances in Veterinary Science, 11, 321-379.PubMedGoogle Scholar
  4. Confer, AW., 2009. Update on bacterial pathogenesis in BRD, Animal Health Research Review, 10,145-1488.CrossRefGoogle Scholar
  5. Cowan, S.T. and Steel, J.K., 1993. Manual for identification of medical bacteria. 3 rd ed. /edited and rev.by G.I.Barrow and R.K.A.Feltham. London, UK: The Syndics of the Cambridge University Press.Google Scholar
  6. Cowart, R.P., Backstrom, L. and Brim, T. A., 1989. Pasteurella multocida and Bordetella bronchiseptica in Atrophic Rhinitis and Pneumonia in Swine, Canadian Journal of Veterinary Research 53, 295-300.PubMedGoogle Scholar
  7. Cruickshank, R., Dugidn, J.P., Marmoin, B.P. and Swain, R.H.A., 1975. Medical Microbiology, vol. 2, 12th edn., Edinburg, London and New York: Churchill Livingstone.Google Scholar
  8. CLSI/ Clinical and Laboratory Standards Institute, 2008. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 3 rd edn. Approved standard M31-A3. Clinical and Laboratory Standards Institute, Wayne, PA.Google Scholar
  9. Davies, R.L., MacCorquodale, R., Baillie, S. and Caffrey, B., 2003. Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis, Journal of Medical Microbiology, 52, 59-67.PubMedCrossRefGoogle Scholar
  10. De Alwis, M.C.L., 1996. Haemorrhagic septicaemia: Clinical and epidemiological features of the disease. Proceedings of International Workshop on Diagnosis and Control of HS, Bali, Indonesia, May 28-30.Google Scholar
  11. De Jong, M.F., Oel, H.L. and Tetenburg, G.J., 1980. Atrophic rhinitis—pathogenicity tests for P. multocida isolates. Proceedings of International Pig Veterinary Society Congress (Copenhagen), p.211.Google Scholar
  12. Dey, S., Singh, V.P., Kumar, A.A., Srivastava, S.K. and Singh, N., 2007. Molecular identification of Indian isolates of P. multocida serotype B:2 by different PCR assays, Indian Journal of Animal Sciences, 77, 317-319.Google Scholar
  13. Dev Sharma, S.P., Sharma, R.K. and Rahman, H., 2004. Isolation, serotyping and antibiogram of Pasteurella from pigs of North East India, Indian Journal of Animal Sciences, 74, 905-907.Google Scholar
  14. Djordjenic, S.P., Eamens, G.J., Ha, H., Walker, M.J. and Chin, Jc., 1998. Demonstration that Australian Pasteurella multocida isolates from sporadic outbreaks of porcine pneumonia are non-toxigenic (toxA) and display heterogeneous DNA restriction endonuclease profiles compared with toxigenic isolates from herds with progressive atrophic rhinitis, Journal of Medical Microbiology, 47,679-688.CrossRefGoogle Scholar
  15. Guillet, C., Lambert, O.J., Carbonnelle, E., Ferroni, A. and Vachee, A., 2007. Pasteurella multocida sepsis and meningitis in 2-month-old twin infants after household exposure to a slaughtered sheep, Clinical Infectious Diseases, 45, e80-1.PubMedCrossRefGoogle Scholar
  16. Iwamatsu, S. and Sawada, T., 1988. Relationship between serotypes, dermonecrotic toxin production of P. multocida isolates and pneumonic lesions of porcine lungs, Japan Journal of Veterinary Sciences, 50, 1200-1206.CrossRefGoogle Scholar
  17. Kalorey, D.R., Yuvaraj, S., Vanjari, S.S., Gunjal, P.S., Dhanawade, N.B., Barbuddhe, S.B., and Bhandarkar, A.G., 2008. PCR analysis of Pasteurella multocida isolates from an outbreak of pasteurellosis in Indian pigs, Comparative Immunology, Microbiology and Infectious Diseases, 31, 459-465.PubMedCrossRefGoogle Scholar
  18. Kehrenberg, C. and Schwarz, S., 2005. dfrA20, a novel trimethoprim resistance gene from Pasteurella multocida, Antimicrobial Agents and Chemotherapy, 49, 414-417.PubMedCrossRefGoogle Scholar
  19. Kehrenberg, C. and Schwarz, S., 2007. Mutations in 16S rRNA and ribosomal protein S5 associated with high level spectinomycin resistance in Pasteurella multocida, Antimicrobial Agents and Chemotherapy, 51, 2244-2246.PubMedCrossRefGoogle Scholar
  20. Kehrenberg, C., Tanzil, G.C., Martel, J.L., Dancla, E.C. and Schwarz, S., 2001. Antimicrobial resistance in Pasteurella and Manhaemia: epidemiology and genetic basis, Veterinary Research, 32, 323-339.PubMedCrossRefGoogle Scholar
  21. Khan, M.A., Yamin, M., Khan, M.S. and Khan, A.G., 1994. Epidemiological and economical based ranking order of buffalo and cattle diseases through active disease surveillance system, Proceedings of the 8th International Congress on Animal Hygiene, St. Paul, Minnesota, USA.Google Scholar
  22. Kumar, P., Singh, V.P., Agrawal, R.K. and Singh, S., 2009., Identification of Pasteurella multocida isolates of ruminant origin using polymerase chain reaction and their antibiogram study, Tropical Animal Health and Production, 41,573-578.PubMedCrossRefGoogle Scholar
  23. Lichtensteiger, C.A., Steenbergen, S.M., Lee, R.M., Polson, D.D. and Vimr, E.R., 1996. Direct PCR analysis for toxigenic Pasteurella multocida, Journal of Clinical Microbiology, 34,3035-3039.PubMedGoogle Scholar
  24. Oehler, R.L., Velez, A.P, Mizrachi, M., Lamarche, J. and Gompf, S., 2009. Bite-related and septic syndromes caused by cats and dogs, Lancet Infectious Disease, 9439-447.Google Scholar
  25. Perrin, F., Renard, B., Belliard, G. and Bontemps, F., 2011. Pasteurella multocida meningoencephalitis, Presse Medicale, 40,212-214.CrossRefGoogle Scholar
  26. Pijoan, C., 1992. Pneumonic Pasteurellosis. In: Leman, A. (eds) Diseases of Swine. 7th edn. Iowa, Wolfe Publishing Ltd. pp.552-559.Google Scholar
  27. Pors, S.E., Hansen, M.S., Christensen, H., Jensen, H.E., Petersen, A. and Bisgaard, M., 2011. Genetic diversity and associated pathology of Pasteurella multocida isolated from porcine pneumonia, Veterinary Microbiology, 150, 354-361PubMedCrossRefGoogle Scholar
  28. Radostits, O. M., Gay, C.C., Hinchcliff, K. W. and Constable, P.D., 2007. Veterinary medicine. 10th edn., Saunders, Elsevier, London, pp.922.Google Scholar
  29. RimIer, R.B. and Rhoades, K.R., 1987. Serogroup F, a new capsule serogroup of Pasteurella multocida, Journal of Clinical Microbiology, 25, 615-618.Google Scholar
  30. Rubies, X., Casal, J., Fernandez ,J. and Pijoan ,C., 1996. Transmission of P. multocida clones in a swine pyramid structure, Proceedings of International Pig Veterinary Society Congress, 14, 243.Google Scholar
  31. Saxena, M.K., Kumar, A.A., Chaudhari, P., Shivachandra, S.B., Singh, V.P. and Sharma, B., 2005. Ribotyping of Indian isolates of Pasteurella multocida based on 16S and 23S rRNA genes, Veterinary Research Communications, 29, 527-535.PubMedCrossRefGoogle Scholar
  32. Sellyei, B., Varga, Z., Szentesi-Samu, K., Kaszanyitzky, E. and Magyar, T., 2009. Antimicrobial susceptibility of Pasteurella multocida isolated from swine and poultry, Acta Veterinaria Hungarica, 57, 357-367.PubMedCrossRefGoogle Scholar
  33. Sharma, M., Dhar, P., Kumar, R., Kapoor, V., Gupta, D., Verma, L. and Katoch, R.C., 2010. Drug sensitivity profile of various capsular types of Pasteurella multocida in Himachal Pradesh, Indian Journal of Animal Sciences, 80, 1066-1069.Google Scholar
  34. Shayegh, J., Sharaf, J.D., Mikaili, P. and Namvar, H., 2009. Pheno and genotyping of Pasteurella multocida isolated from goat in Iran, African Journal of Biotechnology, 8, 3707-3710.Google Scholar
  35. Shivachandra, S.B., Viswas, K.N. and Kumar, A.A., 2011. A review of hemorrhagic septicemia in cattle and buffalo, Animal Health Research Review, 12, 67-82.CrossRefGoogle Scholar
  36. Snedecor, G.W. and Cochran, W.G., 1994. Statistical methods. Iowa State University Press, Ames, Iowa. pp.76-78.Google Scholar
  37. Stillaert, F.B., De Baerdemaeker, L., De Waele, J.J., Claeys, G., Blondeel, P.N. and Monstrey, S., 2010. Necrotizing fasciitis complicated by fatal multiple organ dysfunction due to Pasteurella multocida infection: a case report, Acta Clinica Belgica, 65, 412-414.PubMedGoogle Scholar
  38. Straw, B.E., Zimmerman, J.J., Allaire, S.D. and Tylor, D.J., 2006. Diseases of Swine. 9th edn. Blackwell Publishing.Google Scholar
  39. Tasneem, K., Zamir, B., Ali, S., Gill, Z.J. and Raza, A., 2009. Haemorrhagic septicaemia a review: Pakistan Journal of Science, 61, 10-13.Google Scholar
  40. Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J. and Adler, B., 2001. Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system, Journal of Clinical Microbiology, 39,924-929.PubMedCrossRefGoogle Scholar
  41. Townsend, K.M., Frost, A.J., Lee, C.W., Papadimitriou, J.M. and Dawkins, H.J., 1998. Development of PCR assays for species and type-specific identification of Pasteurella multocida isolates, Journal of Clinical Microbiology, 36,1096-1100.PubMedGoogle Scholar
  42. Varshney, M., Taku, A.K., Dutta, T.K. and Chhabra, R., 2009. Molecular detection of Pasteurella multocida by species and capsular type B specific PCR assay, Indian Journal Veterinary Research, 18, 48-54.Google Scholar
  43. Vera Lizarazo, Y.A., Rodríguez Ferri, E. F. de la Fuente, A. J. M. and Gutiérrez Martín, C. B., 2006. Evaluation of changes in antimicrobial susceptibility patterns of Pasteurella multocida subsp multocida isolates from pigs in Spain in 1987–1988 and 2003–2004, American Journal of Veterinary Research, 67, 663-668.CrossRefGoogle Scholar
  44. Verma, S.C., Mahajan, N.K., Malik, G. and Dahiya, J. P., 2004. An epidemiological study on bovine haemorrhagic septicaemia in Haryana, Indian Journal of Animal Research, 38, 14-19.Google Scholar
  45. Wilson, K., 1987. Preparation of genomic DNA from bacteria. In: Ausubel F.M., Bernt, R. Kingston, R.E., Moore D.D., Seidman S.J., Smith J.A. & Srahul K., (eds). Current protocol in molecular biology, vol. I. pp.241-242, New York: Wiley.Google Scholar
  46. Zhao, G., Pijoan, C. and Murtaugh, M.P., 1992. Epidemiology of Pasteurella multocida in a farrow-to-finish swine herd, Canadian Journal of Veterinary Research, 57, 136-138.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Swaraj Rajkhowa
    • 1
    Email author
  • Ingudam Shakuntala
    • 2
  • Seema Rani Pegu
    • 1
  • Rajib Kumar Das
    • 2
  • Anubrata Das
    • 1
  1. 1.National Research Centre on PigIndian Council of Agricultural Research (ICAR)GuwahatiIndia
  2. 2.ICAR Research Complex for NEH RegionUmiamIndia

Personalised recommendations