Tropical Animal Health and Production

, Volume 44, Issue 4, pp 911–914

Diagnosis of paratuberculosis in goats by cell mediated immune response, conventional and molecular diagnostic techniques

  • Sulficar Shamsudeen Rawther
  • Mapranath Raghavan Saseendranath
  • Govinda Pilla Krishnan Nair
  • Palathingal Vareid Tresamol
  • Usha Narayana Pillai
  • John Abraham
  • Tuticorin Maragatham Alagesan Senthilkumar
  • Shrine Nagalakshmy
  • Krishna Kripa Narayanan Nimisha
Original Research

DOI: 10.1007/s11250-011-9986-y

Cite this article as:
Rawther, S.S., Saseendranath, M.R., Nair, G.P.K. et al. Trop Anim Health Prod (2012) 44: 911. doi:10.1007/s11250-011-9986-y
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Abstract

In the present study efficacy of single intradermal Johnin test, acid fast staining of faecal smear and IS 900 faecal polymerase chain reaction tests was evaluated in 200 goats for detection of Mycobacterium avium subsp paratuberculosis. Two hundred goats comprising 150 goats from an organised farm in Trichur district and 50 goats reared under field condition at farmers premise from Malappuram district of Kerala state formed the study population. Faecal smear from all the 200 goats was stained by Ziehl–Neelsen acid fast stain and faecal polymerase chain reaction (PCR) specific for M. avium subsp paratuberculosis (MAP); IS 900 was performed on all samples. All the animals were subjected to single intradermal Johnin test. Out of 200 goats screened for paratuberculosis, six goats (3%), 11 goats (5.5%) and 42 goats (21%) were found positive by Ziehl–Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS 900 PCR respectively. Results of the present study indicate that amplification of IS 900 insertion element was the most specific and sensitive diagnostic detection method. Single intradermal Johnin test and Ziehl–Neelsen acid fast staining did not show any significant difference.

Keywords

Mycobacterium avium subsp paratuberculosis IS 900 faecal PCR Single intradermal Johnin Ziehl–Neelsen acid fast staining 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Sulficar Shamsudeen Rawther
    • 1
  • Mapranath Raghavan Saseendranath
    • 1
  • Govinda Pilla Krishnan Nair
    • 2
  • Palathingal Vareid Tresamol
    • 1
  • Usha Narayana Pillai
    • 3
  • John Abraham
    • 4
  • Tuticorin Maragatham Alagesan Senthilkumar
    • 5
  • Shrine Nagalakshmy
    • 5
  • Krishna Kripa Narayanan Nimisha
    • 1
  1. 1.Department of Veterinary Epidemiology and Preventive MedicineCollege of Veterinary and Animal Sciences, MannuthyTrichurIndia
  2. 2.Department of Veterinary MicrobiologyCollege of Veterinary and Animal Sciences, MannuthyTrichurIndia
  3. 3.Department of Veterinary Clinical Medicine, Jurisprudence and Ethics, College of Veterinary and Animal Sciences, MannuthyKerala Agricultural UniversityTrichurIndia
  4. 4.Department of Livestock Production Management, College of Veterinary and Animal Sciences, Pookot, WayanadKerala Agricultural UniversityTrichurIndia
  5. 5.Department of Animal BiotechnologyMadras Veterinary CollegeChennaiIndia
  6. 6.Department of Veterinary Epidemiology and Preventive MedicineVeterinary College and Research InstituteNamakkalIndia

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