The objective of this study was to analyze and investigate the genotype frequency and the association between Acyl-CoA:diacylglycerol acyltransferase1 gene, DGAT1 gene, and milk yield (MY), milk composition, protein yield (PY), fat yield (FY), solid not fat yield (SNF), total solid (TS), the content of fat, protein, solid not fat, and total solid, (%Fat,%Prot,%SNF,%TS) in two herds of crossbred Holstein dairy cattle in Thailand. Two hundred and twenty-seven crossbred Holstein cows were used and their blood samples were taken for the study. PCR–RFLP was used to identify the allele and genotype of DGAT1 gene. A general linear model and the least square method were used to estimate the least square mean and additive, and the dominant effect of the gene on the traits and the least significant differences were used to compare the mean of each trait between genotypes. Two alleles (K, A) and three genotypes (AA, KA, KK) were detected, the highest allele and genotype frequencies were A and AA, respectively. The least mean squares of each genotype were compared and significant differences between genotype were detected. Genotype KK has the greatest effect on all milk composition content traits, while genotype AA has the greatest effect on yield traits. Highly significant additive gene effect was detected. From the results, it can be concluded that the DGAT1 gene can be used as a gene marker for assisted selection in milk composition traits.
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Banos, G., Woolliams, J.A., Woodward, B.W., Forbes, A.B., Coffey, M.P., 2008. Impact of single nucleotide polymorphisms in leptin, leptin receptor, growth hormone receptor, and diacylglycerol acyltransferase (DGAT1) gene loci on milk production, feed, and body energy traits of UK dairy cows. Journal Dairy Science, 91, 3190–3200.
Barbosa D. S. M.V, Sonstegars, T.S.,, Thallman, R.M., Connor, E.E., Schnabel, R.D., Van Tassell. C,P.2010. Characterization of DGAT1 allelic effects in a sample of North American Holstein cattle. Animal biotechnology. 21, 88–99.
Bennewitz, J., Reinsch, N., Paul, S., Looft, C., Kaupe, B., Weimann, C., Erhardt, G., Thaller, G., Kuhn, C., Schwerin, M., Thomsen, H., Reinhardt, F., Reents, R., Kalm, E., 2004. The DGAT1 K232A mutation is not solely responsible for the milk production quantitative trait locus on the bovine chromosome 14. Journal Dairy Science, 87, 431–442.
Cardoso, S.R., Queiroz, L.B., Alonso, V., Goulart, G.B., Mouro, E., Benedetti, L., Goulart, R. 2011. Productive performance of the dairy cattle Girolando breed mediated by the fat-related genes DGAT1 and LEP and their polymorphisms. Research in Veterinary Science. Article in press.
Falconer, D.S., Mackay, T.F.C. 1996. Introduction to quantitative genetics, 4th. Longman Scientific and Technical. New York.
Freitas, A. F., Wilcox, C. J., Costa, C.N., 1998. Breed group effects on milk production of Brazilian crossbred dairy cows. Journal Dairy Science 81, 2306–2311.
Gautier, M., Capitan, A., Fritz, S., Eggen, A., Boichard, D., Druet, T., 2007. Characterization of the DGAT1 K232A and variable number of tandem repeat polymorphisms in French dairy cattle. Journal Dairy Science, 90, 2980–2988.
Grisart, B., Coppieters,W., Farnir, F., Karim, L., Ford, C., Berzi, P., Cambisano, N., Mni, M., Reid, S., Simon, P., Spelman, R., Georges, M., Snell, R., 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research, 12(2), 222–231.
Hammami, H., Rekik, B., Bastin, C., Soyeurt, H., Bormann, J., Stoll, J., Gengler, N., 2009. Environmental sensitivity for milk yield in Luxembourg and Tunisian Holsteins by herd management level. Journal Dairy Science, 92, 4604–4612.
Hayes, B. J., Carrick, M., Bowman, P., Goddard, M.E., 2003. Genotype × environment interaction for milk production of daughters of Australian dairy sires from test-day records. Journal Dairy Science, 86, 3736–3744.
Kaupe, B., Winter, A., Fries, R., Erhardt, G., 2004. DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. Journal of Dairy Research, 71, 182–187.
Kuehn, C., Edel, C., Weikard, R., Thaller, G., 2007. Dominance and parent-of-origin effects of coding and non-coding alleles at the acylCoA-diacylglycerol-acyltransferase (DGAT1) gene on milk production traits in German Holstein cows. BMC Genetics, 8, 62–70.
Lacorte, G.A., Machado, M.A., Martinez, M.L., Campos, A.L., Maciel, R.P., Verneque, R.S., Teodoro, R.L., Peixoto, M.G.C.D., Carvalho, M.R.S., Fonseca, C.G., 2006. DGAT1 K232A polymorphism in Brazilian cattle breeds. Genetics and Molecular Research, 5, 475–482.
Lillehammer, M., Hayes, B.J., Meuwissen, T. H. E., Goddard, M. E., 2009. Gene by environment interactions for production traits in Australian dairy cattle Journal Dairy Science, 92,4008–4017.
Meuwissen, T. H. E., Van Arendonk., J. A. M., 1992. Potential improvements in rate of genetic gain from marker assisted selection in dairy cattle breeding schemes. Journal Dairy Science, 75, 1651–1659.
Milazzotto, M.P., Rahal, P., Nichi, M., Miranda-Neto, T., Teixeira, L.A., Ferraz, J.B.S., Eler, J.P., Campagnari, F., Garcia, J.F., 2008. New molecular variants of hypothalamus–pituitary–gonad axis genes and their association with early puberty phenotype in Bos taurus indicus (Nellore). Livestock Science, 114, 274–279.
Naslund, J., Fikse, W.F., Pielberg, G.R., Lunden, A., 2008. Frequency and effect of the bovine acyl–CoA:diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism in Swedish dairy cattle. Journal Dairy Science, 91 (5), 2127–2134.
Nowacka-Woszuk, J., Noskowiak, A., Strabel, T., Jankowski, T., Świtoński M., 2008. An effect of the DGAT1 gene polymorphism on breeding value of Polish Holstein-Friesian sires. Animal Science Papers and Reports, 26, 17–23.
Pareek, C.S., Czarnik, U., Zabolewicz, T., Pareek, R.S., Walawski, K., 2005. DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle. Journal of Applied Genetics, 46, 85–87.
Peterson, D.G., Matitashvili, E.A. Bauman, D.E., 2003. Diet-induced milk fat depression in dairy cows results in increased trans-10, cis-12 CLA in milk fat and coordinate suppression of mRNA abundance for mammary enzymes involved in milk fat synthesis. Journal Nutrition, 133, 3098–3102.
Raymond, M., Rousset, F., 2003. Genepop 3.4., an updated version of Genepop V.1.2 (1995): Population genetics software for exact tests and ecumenicism. Journal of Heredity, 86, 248–9.
Sanders, K., Bennewitz, J., Reinsch N., Thaller, G., Prinzenberg, E.M., Kühn, C., Kalm, E., 2006. Characterization of the DGAT1 mutations and the CSN1S1 promoter in the German Angeln dairy cattle population. Journal Dairy Science, 89, 3164–3174.
Schennink,A., Stoop,W.M., Visker,M.H., Heck, J.M., Bovenhuis,H., van der Poel, J.J., van Valenberg, H.J., van Arendonk, J.A., 2007. DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Animal Genetics, 38 (5), 467–473.
Signorelli, F, Orrù, L., Napolitano, F., Matteis, G.D., Scatà, M.C., Catillo, G., Marchitelli, C., Moioli, B., 2009. Exploring polymorphisms and effects on milk traits of the DGAT1, SCD1 and GHR genes in four cattle breeds. Livestock Science, 125, 74–79.
Smaragdov M.G., 2011. Association of the DGAT1 gene polymorphism in bull with cow milk performance. Animal Genetics, 47, 126–132.
Spelman, R.J., Ford, C.A., McElhinney, P., Gregory, G.C., Snell, R.G., 2002. Characterization of the DGAT1 gene in New Zealand dairy population. Journal Dairy Science, 85, 3514–3517.
Streit, M., Neugebauer, N., Meuwissen, T.H., Bennewitz, J. 2011. Evidence for a major gene by polygene interaction for milk production traits in German Holstein dairy cattle. Journal Dairy Science, 94,1597–6000.
Strzalkowska, N., Siadkowska, E., Sloniewski, K., Krzyzewski, J., Zwierzchowski, L., 2005. Effect of DGAT1 gene polymorphism on milk production traits in Black- and -White (Friesian) cows. Animal Science Papers and Report, 23, 189–197.
Sun, D.,Jia, J., Ma, Y.,Zhang, Y., Wang, Y., Yu, Y., Zhang, Y., 2009 Effects of DGAT1 and GHR on milk yield and milk composition in the Chinese dairy population. Animal Genetics, 40, 997–1000.
Tadesse, M., Dessie., T., 2003. Milk production performance of Zebu, Holstein Friesian and their crosses in Ethiopia. Livestock Research for Rural Development, 15. http://www.cipav.org.co/lrrd/lrrd15/3/Tade153.htm (04/03/06)
Thaller, G., Kramer, W., Winter, A., Kaupe, B., Erhardt, G., Fries, R., 2003. Effects of DGAT1 variants on milk production traits in German cattle breeds. Journal of Animal Science, 81, 1911–1918.
Weller, J. I., Golik, M., Seroussi, E., Ezra, E., Ron, M., 2003. Population-wide analysis of a QTL affecting milk-fat production in the Israeli Holstein population Journal Dairy Science, 86, 2219–2227.
Winter, A., Kramer, W., Werner, F., Kollers, S., Kata, S., Durstewits, G., Buitkamp, J., Womack, J.E., Thaller, G., Fries, R., 2002. Association of a lysine-232/alanine polymorphism in a bovine gene encoding diacylglycerol acyltransferase (DGAT) with variation at a quantitative trait locus for on milk fat content in cattle. Proceedings of the National Academy of Sciences, 99, 9300–9305.
The authors are grateful to Chiyasarn Dairy Cattle Farm and the Farm of Suranaree University of Technology for providing the data and blood collection, to Dr. Thanathip Suwanasopee for useful suggestions, and Suranaree University of Technology for providing funds.
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Molee, A., Duanghaklang, N. & Na-Lampang, P. Effects of Acyl-CoA:diacylglycerol acyl transferase 1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle. Trop Anim Health Prod 44, 751–755 (2012). https://doi.org/10.1007/s11250-011-9959-1
- Acyl-CoA:diacylglycerol acyl transferase 1 gene
- Crossbred Holstein cattle
- Milk composition
- Milk production