Tropical Animal Health and Production

, Volume 42, Issue 8, pp 1649–1654 | Cite as

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

  • Fikru Regassa Gari
  • Hagos Ashenafi
  • Alemu Tola
  • Bruno M. Goddeeris
  • Filip Claes
Original Research


Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P > 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P < 0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair \( \left( {k = 0.{1} - 0.{4}} \right) \) with significantly higher sensitivity by PCR than other tests.


Dourine Horses Prevalence and test agreement 



This study was supported by VLIR (Flemish Interuniversity Council) of Belgium. We would like to thank the Dean of Faculty of Veterinary Medicine, Addis Ababa University Ethiopia, Professor Merga Bekana for his support during the research. F. Claes is a postdoctoral fellow of the Flanders Research Foundation (FWO).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Fikru Regassa Gari
    • 1
  • Hagos Ashenafi
    • 1
  • Alemu Tola
    • 1
  • Bruno M. Goddeeris
    • 2
  • Filip Claes
    • 2
    • 3
  1. 1.Faculty of Veterinary MedicineAddis Ababa UniversityDebre ZeitEthiopia
  2. 2.Division Gene Technology, Department of Biosystems, Faculty of Bioscience EngineeringKatholieke Universiteit LeuvenLeuvenBelgium
  3. 3.Department of ParasitologyInstitute of Tropical MedicineAntwerpBelgium

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