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Tropical Animal Health and Production

, Volume 42, Issue 8, pp 1649–1654 | Cite as

Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

  • Fikru Regassa Gari
  • Hagos Ashenafi
  • Alemu Tola
  • Bruno M. Goddeeris
  • Filip Claes
Original Research

Abstract

Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P > 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P < 0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair \( \left( {k = 0.{1} - 0.{4}} \right) \) with significantly higher sensitivity by PCR than other tests.

Keywords

Dourine Horses Prevalence and test agreement 

Notes

Acknowledgements

This study was supported by VLIR (Flemish Interuniversity Council) of Belgium. We would like to thank the Dean of Faculty of Veterinary Medicine, Addis Ababa University Ethiopia, Professor Merga Bekana for his support during the research. F. Claes is a postdoctoral fellow of the Flanders Research Foundation (FWO).

References

  1. Alemu, T., Luckins, A.G., Phipps, L.P., Reid, S.W.J., Holmes, PH., 1997. The use of Enzyme Linked Immunosorbent Assays to investigate the prevalence of Trypanosoma equiperdum in Ethiopian horses, Veterinary Parasitology, 71, 239-250.CrossRefPubMedGoogle Scholar
  2. Arsi-Bale Zone Agricultural and Rural Development Office, 2009. Annual Meteorological and livestock report, Robe, Ethiopia.Google Scholar
  3. Bajyana Songa, E., Hamers, R., 1988. A card agglutination test (CATT) for veterinary use based on an early VAT RoTat 1/2 of Trypanosoma evansi, Annual Society of Belgian Tropical Medicine, 68, 233-240.Google Scholar
  4. Barrowman, P. R., 1976. Observations on the transmission, immunology, clinical signs and chemotherapy of dourine (Trypanosoma equiperdum infection) in horses, with special reference to cerebro-spinal fluid. Onderstepoort Journal of Veterinary Research, 43 (2), 55-66.PubMedGoogle Scholar
  5. Claes, F., Agbo, E. C., Radwanska, M., Tepas, M. F. W., Baltz, T., De Waal, D. T., Goddeeris, B. M., Claassen, E., Büscher, P., 2003. How does Trypanosoma equiperdum fit into the Trypanozoon group? A cluster analysis by RAPD and Multiplex-endonuclease genotyping approach. Parasitology, 126, 425-431.CrossRefPubMedGoogle Scholar
  6. Claes, F., Radwanska, M. Urakawa, T., Majiwa, P.A., Goddeeris, B., Büscher, P., 2004. Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections. Kinetoplastid Biol Dis, 3, 3CrossRefPubMedGoogle Scholar
  7. Clausen, P-H., Gebreselassie, G., Abditcho, S., Mehlitz, D., Staak, C., 1999. Detection of trypanosome DNA in serologically positive but aparasitaemic horses suspected of dourine in Ethiopia. Tokai Journal of experimental and Clinical Medicine, 23 (6), 303-308.Google Scholar
  8. Clausen, P-H., Chuluun, S., Sodnomdarjaa, R., Greiner, M., Noeckler, K., Staak, C., Zessin, K-H., Schein, E., 2003. A field study to estimate the prevalence of Trypanosoma equiperdum in Mongolian horses, Veterinary Parasitology, 115(1), 9-18CrossRefPubMedGoogle Scholar
  9. CSA., 2008. Central Statistical Authority. Addis Ababa, Ethiopia.Google Scholar
  10. Dagnachew, Z., Shafo, K., Kelil, A., 1980. An investigation of dourine in Arsi administrative region. Ethiopian Medical Journal, 4, 3-19Google Scholar
  11. Desquesnes, M., Dávila, A. M. R., 2002. Applications of PCR-based tools for detection and identification of animal trypanosomes. A review and perspectives. Veterinary Parasitology, 109, 213–231CrossRefPubMedGoogle Scholar
  12. Hagos, A., Abebe, G., Buscher, P., Goddeeris, B. M. and Claes, F., 2010. Serological and parasitological survey of dourine in the ArsiBale highlands of Ethiopia. Tropical Animal Health and Production, 42 (4), 769CrossRefPubMedGoogle Scholar
  13. Hilali, M., Abdel-Gawad, A., Nassar, A., Abdel-Wahab, A., Magnus, E., Büscher, P., 2004. Evaluation of the Card Agglutination Test (CATT/T. evansi) for detection of Trypanosoma evansi infection in water buffaloes (Bubalus bubalis) in Egypt. Veterinary Parasitology, 121 (1-2), 45-51CrossRefPubMedGoogle Scholar
  14. Luckins, A. G., 1994. Equine trypanosomiasis. Exotic disease series. Equine Veterinary Education, 6 (5), 259-262CrossRefGoogle Scholar
  15. Maarten, P., 2009. Role and importance of equines and constraints of equine keeping in the Arsi-Bale Highlands of Ethiopia. MSc thesis, Catholic University Leuven.Google Scholar
  16. Ngaira, J. M., Njagi, E. N. M., Ngeranwa, J. J. N., Olembo, N. K., 2004. PCR amplification of RoTat 1.2 VSG gene in Trypanosoma evansi isolates in Kenya, Veterinary Parasitology, 120, 23-33CrossRefPubMedGoogle Scholar
  17. Ngaira, J. M., Olembo, N. K., Njagi, E. N. M., Ngeranwa J. J. N., 2005. The detection of non-RoTat 1.2 Trypanosoma evansi, Experimental Parasitology, 110, 30-38CrossRefPubMedGoogle Scholar
  18. QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook, 2003. QIAGEN GmbH, QIAGEN Strasse 1. 40724.Hilden, Germany.Google Scholar
  19. Reid, S. A., Husein, A., Copeman, D. B., 2001. Evaluation and improvement of parasitological tests for Trypanosoma evansi infection. Veterinary Parasitology, 102(4), 291-297CrossRefPubMedGoogle Scholar
  20. Stuart, K., Brun, R., Croft, S., Fairlam, A., Gürtler, R. E., McKerrow, J., Reed, S., Tarleton, R., 2008. Kinetoplastids: related protozoan pathogens, different diseases, Journal of Clinical Investigation. 118(4), 1301-1310.CrossRefPubMedGoogle Scholar
  21. Urakawa, T., Verloo, D., Moens, L., Buscher, P., Majiwa, P. A. O., 2001. Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2. Experimental Parasitology, 99, 181-189CrossRefPubMedGoogle Scholar
  22. Verloo, D., Holland, W., My, L. N., Thanh, N. G., Tam, P. T., Goddeeris, B., Vercruysse, J., Büscher, P., 2000. Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from North Vietnam, Veterinary Parasitology, 92, 87-96CrossRefPubMedGoogle Scholar
  23. Viera, A. J., Garrett, J. M., 2005. Understanding Inter observer Agreement: The Kappa Statistic. Family Medicine, 37(5), 360-363PubMedGoogle Scholar
  24. Woo, P. T. K., 1969.The haematocritcentrifuge for the detection of trypanosomes in blood. Canadian Journal of Zoology, 47(5), 921-923CrossRefPubMedGoogle Scholar
  25. Zablotskij, V. T., Georgiu, C., De Waal, T., Clausen, P. H., Claes, F. and Touratier, L., 2003. The current challenges of dourine: difficulties in differentiating Trypanosoma equiperdum within the subgenus Trypanozoon. Review Scientific Techniques Office International Epizootices, 22, 1087-1096.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Fikru Regassa Gari
    • 1
  • Hagos Ashenafi
    • 1
  • Alemu Tola
    • 1
  • Bruno M. Goddeeris
    • 2
  • Filip Claes
    • 2
    • 3
  1. 1.Faculty of Veterinary MedicineAddis Ababa UniversityDebre ZeitEthiopia
  2. 2.Division Gene Technology, Department of Biosystems, Faculty of Bioscience EngineeringKatholieke Universiteit LeuvenLeuvenBelgium
  3. 3.Department of ParasitologyInstitute of Tropical MedicineAntwerpBelgium

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