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Porcine parvovirus- and porcine circovirus 2-associated reproductive failure and neonatal mortality in crossbred Indian pigs

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Abstract

The objective of this study was to detect the presence of porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in a farm showing reproductive failure and increased mortality in neonatal piglets by histopathological examination, polymerase chain reaction, and demonstration of viral antigen and nucleic acid. Out of 594 piglets farrowed by 70 first-parity gilts, nine (1.51%) mummified fetuses, 13 (2.19%) stillborn, and 572 (96.3%) live-born piglets were recorded. The average litter size at birth was 8.48 piglets per litter. One hundred ninety-four (33.91%) piglets died within 7 days of age. PPV was detected in five litters (7.14%) and two of them revealed coinfection with PCV2. The pathological lesions in the coinfected litters were more severe, indicating a synergistic action between the two viruses. Results of this study suggest for the first time occurrence of PPV and coinfection with PCV2 in crossbred Indian pigs affected with reproductive problem and neonatal mortality.

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Abbreviations

APES:

3-Aminopropyl triethoxysilane

DAB:

Diaminobenzidine

DIG:

Digoxigenin

HE:

Hematoxylin and eosin

HRPO:

Horseradish peroxidase

ISH:

In situ hybridization

NBT–BCIP:

Nitro blue tetrazolium and 5′bromocresyl-3-indoylphosphate

PALS:

Periarteriolar lymphoid sheath

PCV2:

Porcine circovirus 2

PPV:

Porcine parvovirus

PRRSV:

Porcine reproductive and respiratory syndrome virus

RT:

Room temperature

RE:

Reticuloendothelial

SSC:

Sodium chloride sodium citrate

TBE:

Tris–borate–EDTA

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Acknowledgments

The authors acknowledge Director, IVRI, Izatnagar for providing necessary facilities during the course of this investigation.

Author information

Correspondence to G Saikumar.

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Sharma, R., Saikumar, G. Porcine parvovirus- and porcine circovirus 2-associated reproductive failure and neonatal mortality in crossbred Indian pigs. Trop Anim Health Prod 42, 515–522 (2010). https://doi.org/10.1007/s11250-009-9454-0

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Keywords

  • Pigs
  • Porcine parvovirus
  • Porcine circovirus 2
  • Coinfection
  • India