Tropical Animal Health and Production

, Volume 42, Issue 3, pp 457–464 | Cite as

Evaluation of a 3% surf solution (surf field mastitis test) for the diagnosis of subclinical bovine and bubaline mastitis

  • Ghulam Muhammad
  • Abeera Naureen
  • Muhammad Nadeem Asi
  • Muhammad Saqib
  • Fazal-ur-Rehman
Article

Abstract

Purpose

To evaluate a 3% solution of household detergent viz., Surf Excel (Surf field mastitis test, SFMT) vis-à-vis California mastitis test (CMT), Whiteside test (WST), somatic cell counts (SCC; cut off limit = 5 × 105 cells per millilitre) and bacteriological cultures for the detection of subclinical mastitis in quarter foremilk samples (n = 800) of dairy cows and buffaloes.

Methods

Culture and SCC were used as gold standards. All tests were evaluated parallel and serial patterns. The sensitivities of SFMT, SCC, culture, CMT and WST in parallel testing were 72.82, 81.55, 87.38, 75.73 and 54.37%, respectively in cows, while 66.22, 79.73, 82.43, 70.27 and 50.00, respectively in buffaloes.

Results

SFMT was significantly (p < 0.05) more sensitive than WST and comparable to CMT in both species. In serial testing, percent specificity of SFMT (87.12 in cow; 85.16 in buffaloes) was significantly (P < 0.05) higher than that of CMT (83.33 in cow; 80.64 in buffaloes). The negative predictive values of SFMT (93.50 in cow; 96.35 in buffaloes) differed non-significantly from that of CMT (94.02 in cow; 96.15 in buffaloes). The kappa index between the tests was moderate to perfect both in parallel (0.54 to >0.80) and serial (0.58 to >0.8) testing.

Conclusion

On the basis of closely similar diagnostic efficiency of SFMT to CMT in terms of sensitivity, specificity, predictive values and kappa index together with inexpensive and ready availability of SFMT reagent, it tempting to suggest that SFMT can be use as a cheaper, user-friendly alternative animal-side subclinical mastitis diagnostic test in poor countries.

Keywords

Surf field mastitis test Cow Buffalo Subclinical mastitis Test evaluation 

References

  1. Allore, H.G., 1993. A review of incidence of mastitis in buffaloes and cattle, Pakistan Veterinary Journal, 13, 1–7.Google Scholar
  2. Almaw, G., Zerihun, A. and Asfaw, Y., 2008. Bovine mastitis and its association with selected risk factors in smallholder dairy farms in and around Bahir Dar, Ethiopia, Tropical Animal Health Production, 40, 427–432.CrossRefGoogle Scholar
  3. Buragahain, J. and Dutta, G.N., 1991. Comparative efficacy of indirect test for detection of bovine subclinical mastitis, Indian Veterinary Journal, 68:19–22.Google Scholar
  4. Cady, R.A., Shah, S.K., Schermerhorn, E.C. and McDowell, R.E., 1983. Factors affecting performance of Nilli-Ravi buffaloes in Pakistan, Journal of Dairy Science, 66, 578–586.PubMedGoogle Scholar
  5. Dohoo, I.R., 1990. Evaluating mastitis tests. In: Proceeding of 29th Annual Meeting of National Mastitis Council, Inc., Feb. 12-14, 1990 (Louisville Kentucky, USA), 68–77.Google Scholar
  6. Emanuelson, U., Olsson, T., Holmberg, O., Hageltorn, M., Mattila, T., Nelson, L. and Aström, G., 1987. Comparison of some screening test for detecting mastitis, Journal of Dairy Science, 70, 880–887.PubMedGoogle Scholar
  7. Erskine, R.J., Wagner, S.A. and DeGraves, F.J., 2003. Mastitis therapy and pharmacology, Veterinary Clinics of North America—Food Animal Practice, 19, 109–138.CrossRefGoogle Scholar
  8. Fetrow, J., Stewart, S., Eicker, S., Farnworth, R. and Bey, R., 2000. Mastitis: An economical consideration. In: Proceeding of 39th Annual Meeting of National Mastitis Council, February, 13–16, 2000 (Atlanta Georgia, USA), 3–47.Google Scholar
  9. George, L.W. Divers, T.J., Ducharme, N. and Welcome, F.L., 2008. Rebhun’s Diseases of Dairy Cattle (Saunders Elsevier, St Louis, USA).Google Scholar
  10. Getahun, K., Kelay, B., Bekana, M. and Lobago, F., 2008. Bovine mastitis and antibiotic resistance patterns in Selalle smallholder dairy farms, central Ethiopia, Tropical Animal Health Production, 40, 261–168.CrossRefGoogle Scholar
  11. Gulati, S.K., Garg, M.R. and Scott, T.W., 2008. Innovative processes for enhancing milk yield and quality, Pakistan Journal of Agriculture Science, 45, 242–244.Google Scholar
  12. Heeschen, W.H., 2005. Somatic cells as an indicator of milk hygiene: scientific basis and the EU approach. In: Proceeding of 44th Annual Meeting of National Mastitis Council. Jan, 16–19, 2005 (Orlando, Florida, USA), 52–72.Google Scholar
  13. Hussain, M., Malik, M.A., Fatima, Z. and Yousaf, M.R., 2005. Participatory surveillance of livestock diseases in Islamabad capital territory, International Journal of Agriculture and Biology, 7, 657–570.Google Scholar
  14. Klaas, I.C., Enevoldsen, C., Vaarst, M. and Houe, H., 2004. Systematic clinical examination for identification of latent udder health types in Danish dairy herd, Journal of Veterinary Science, 87, 1217–1228.Google Scholar
  15. Martin, S.W., 1977. The evaluation of tests, Canadian Journal of Comparative Medicine, 41, 19–25.PubMedGoogle Scholar
  16. Muhammad, G., Athar, M., Shakoor, A., Khan, M.Z., Fazul-ur-Reham and Ahmad, M.T., 1995. Surf field mastitis test: an inexpensive new tool for evaluation of wholesomeness of fresh milk, Pakistan Veterinary Journal of Food Science, 5, 91–93Google Scholar
  17. National Mastitis Council, Inc., 1987. Laboratory and Field Handbook on Bovine Mastitis, National Mastitis Council, Inc., Arlington, VA, USA.Google Scholar
  18. National Mastitis Council, Inc., 1990. Microbiological Procedures for the Diagnosis of Bovine Udder Infection. National Mastitis Council, Inc., Arlington, VA, USA.Google Scholar
  19. National Mastitis Council Inc., 1999. Laboratory Handbook on Bovine Mastitis. Revised Ed. National Mastitis Council Inc., Madison, Wisconsin, USA.Google Scholar
  20. Naureen, A., Saqib, M., Muhammad, G., Hussain, M. H. and Asi, M. N., 2007. Comparative evaluation of Rose Bengal plate agglutination test, mallein and some conventional serological tests for diagnosis of spontaneous equine glanders, Journal of Veterinary Diagnostic Investigations 19, 362–367.Google Scholar
  21. Ngatia, T.A., Jensen, N.E. and Berg, B.B., 1991. Microscopic changes in infected bovine teat, British Veterinary Journal, 147, 133–139.PubMedGoogle Scholar
  22. Ogola, H., Shitandi, A., Nanua, J., 2007. Effect of mastitis on raw milk compositional quality. Journal Veterinary Science, 8, 237–242.CrossRefGoogle Scholar
  23. Owens, W.E., Ray, C.H., Boddie, R.L. and Nickerson, S.C., 1997. Efficacy of sequential intramammary antibiotic treatment against chronic Staphylococcus aureus intramammary infection, Large Animal Practitioner, 18, 10–14.Google Scholar
  24. Patgiri, G.P., Handique, S.C., Saikai, G.K. and Roychoudhury, R.K., 1987. Comparative efficacy of some indirect tests for the diagnosis of subclinical mastitis in cow, Indian Journal of Veterinary Medicine, 7, 146–147.Google Scholar
  25. Pyörälä, S., 2003. Indicators of inflammation in the diagnosis of mastitis. Veterinary Research, 34, 565–578.CrossRefPubMedGoogle Scholar
  26. Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W., 2007. Veterinary Medicine. W.B. Sanunders Co., Philadelphia. USA.Google Scholar
  27. Ruegg, P.L. and Reinemann, D.J., 2002. Milk quality and mastitis test, Bovine Practitioner, 36, 41–54.Google Scholar
  28. Schalm, O.W., Carroll, G. and Jain, J.C., 1971. Bovine Mastitis, Lea and Febiger, Philadelphia, USA.Google Scholar
  29. Schultz, L.H., Brown, R.W., Jasper, D.E., Mellan Berger, R.W., Natzke, R.P., Philpot, W.N., Smith, J.W. and Thompson, P.D., 1978. Current concept of bovine mastitis (National Mastitis Council, Inc., Washington, D.C., USA).Google Scholar
  30. Shitandi, A. and Kihumbu, G., 2004. Assessment of California mastitis test usage in smallholders dairy herd and risk of violate antimicrobial residues, Journal of Veterinary Science, 5, 5–9.PubMedGoogle Scholar
  31. Silva, I.D. and Silva, K.F. S.T., 1994. Total and differential cell count in buffaloes (Bubalus bubalis) milk, Buffalo Journal, 2, 133–137.Google Scholar
  32. Smith, D.R., 2006. Veterinary Clinical Epidemiology. 3rd Ed. (CRC Press, Taylor & Francis, Boca Raton, Florida, USA.).Google Scholar
  33. Viera, A.J. and Garret J.M., 2005. Understanding inter-observer agreement: the kappa statistic, Family Medicine, 37, 360–363.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ghulam Muhammad
    • 1
  • Abeera Naureen
    • 2
  • Muhammad Nadeem Asi
    • 1
  • Muhammad Saqib
    • 1
  • Fazal-ur-Rehman
    • 3
  1. 1.Department of Clinical Medicine and SurgeryUniversity of AgricultureFaisalabadPakistan
  2. 2.Department of Clinical Medicine and SurgeryUniversity of Veterinary and Animal SciencesLahorePakistan
  3. 3.Livestock and Dairy DevelopmentLahorePakistan

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