Tropical Animal Health and Production

, Volume 42, Issue 3, pp 431–438 | Cite as

Nutritional evaluation of transgenic cottonseed in the ration of lactating dairy cows

  • Ranjan K. Mohanta
  • Kamal K. Singhal
  • Amrish K. Tyagi
  • Y. S. Rajput
  • Shiv Prasad
Article

Abstract

The effects of feeding transgenic (Bt) whole cottonseed (WCS) were studied in lactating cows. Twenty multiparous crossbred cows (Karan Swiss × Karan Fries) in early lactation were given a concentrate mixture containing 40% crushed delinted non-transgenic (non-Bt) WCS, 2 kg wheat straw and green fodder ad lib for a 15-day adaptation period. Thereafter, the cows were divided in two similar groups of 10 each on the basis of milk yield, body weight (BW) and date of calving. The non-Bt control group continued on same ration, while for the Bt group the non-Bt WCS was replaced by transgenic WCS, in a feeding trial of four weeks. The diets provided a minimum of 2 kg cottonseed/cow/d. Mean DMI/100 kg BW and milk yield of non-Bt and Bt groups was 3.48 and 3.45 kg and 11.4 and 12.0 kg/d, respectively. Intake of nutrients, digestibility, milk production and body condition score (BCS) did not differ between the groups (P > 0.05), but BW gain was higher (P < 0.05) in the Bt group than the non-Bt group, probably as a result of hoof problem in two cows of non-Bt group, which when compared excluding two animals from each group did not differ significantly (P > 0.05). Transgenic protein (Cry1C) was not detected in the weekly milk samples or in blood plasma at the end of the experiment, showing that delinted WCS containing Cry1C protein can safely be fed to lactating cows.

Keywords

Bt protein Cottonseed Digestibility Feed intake Transgenic 

References

  1. Alexander, T. W., Reuter, T., Aulrich, K., Sharma, R., Okaine, E. K., Dixon, W. T., and McAllister, T. A. 2007. A review of the detection and fate of novel plant molecules derived from biotechnology in livestock production. Animal Feed Science and Technology, 133, 31–62CrossRefGoogle Scholar
  2. AOAC, 1990. Official Methods of Analysis, 15th edition, Association of Official Analytical Chemists, Arlington, VA, USA.Google Scholar
  3. Arieli, A., 1998. Whole cottonseed in dairy cattle feeding: A review. Animal Feed Science and Technology, 72, 97–110CrossRefGoogle Scholar
  4. CAST (Council for Agricultural Science and Technology), 2006. Safety of meat, milk, and eggs from animals fed crops derived from modern biotechnology, Issue Paper No. 34, CAST, Ames, Iowa, USA. pp 1–8Google Scholar
  5. Castillo, R., Gallardo, M.R., Maciel, M., Giordano, J.M., Conti, G.A., Gaggiotti, M.C., Quaino, O., Gianni, C. and Hartnell, G.F., 2004. Effects of feeding rations with genetically modified whole cottonseed to lactating Holstein cows. Journal of Dairy Science, 87, 1778–1785PubMedGoogle Scholar
  6. Flachowsky, G., Aulrich, K., Bohme, H. and Halle, I., 2007. Studies on feeds from genetically modified plants (GMP)-contributions to nutritional and safety assessment. Animal Feed Science and Technology, 133, 2–30CrossRefGoogle Scholar
  7. Goering, H. K. and Van Soest, P. J., 1967. Forage fibre analysis (Apparatus, reagents, procedures, and some application). Agriculture Handbook No. 379, ARS-USDA, Washington, DC.Google Scholar
  8. Hofte, H. and Whiteley H.R., 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews, 53(2), 242–255PubMedGoogle Scholar
  9. Huls, T.J., Erickson, G.E., Klopfenstein, T.J., Luebbe, M.K., Vander, K.J., Rice, D.W., Smith, B., Hinds, M., Owens, F. and Liebergesell, M., 2008. Effect of feeding DAS-59122-7 corn grain and non-transgenic corn grain to individually fed finishing steers. Professional Animal Scientist, 24, 572–577Google Scholar
  10. IDF, 1984. Recommended methods for somatic cell counting in milk. Bulletin of International Dairy Federation, 168, 4–6Google Scholar
  11. Kaneko, J.J., 1997. Clinical Biochemistry of Domestic Animals, 5th edn, Academic Press, San Diego, CA., USA.Google Scholar
  12. Kumar, R.S. and Singhal, K.K., 2004. Chemical composition and nutritional evaluation of transgenic cottonseed for ruminants. Indian Journal of Animal Sciences, 74 (5), 868–871.Google Scholar
  13. Moreira, V.R., Satter, L.D. and Harding, B., 2004.Comparison of conventional linted cottonseed and mechanically delinted cottonseed in diets for dairy cows. Journal of Dairy Science, 87, 131–138PubMedCrossRefGoogle Scholar
  14. Mujahid, A., Abdullah, M., Barque, A.R. and Gilani, A.H., 2000. Nutritional value of cottonseeds and its derived products: I. Physical fractionations and proximate composition. Asian–Australasian Journal of Animal Sciences, 13, 348–355Google Scholar
  15. NRC, 2001. Nutrient Requirements of Dairy Cattle. 7th revised edition, National Research Council, National Academy of Sciences, Washington, DC, USA.Google Scholar
  16. Prasad, S., 1994. Studies on body condition scoring and feeding management in relation to production performance of crossbred cows. Ph. D. Thesis, National Dairy Research Institute, Karnal, Haryana, India.Google Scholar
  17. Raghuramulu, N, Nair, K.M., Kalyanasundaram, S., 2003. A manual of laboratory techniques. National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, IndiaGoogle Scholar
  18. Ranjhan, S.K., 1998. Nutrient requirement of livestock and poultry, Indian Council of Agricultural Research (ICAR) Publication, New Delhi, India.Google Scholar
  19. Saijpaul, S., Grewal, R.S., Ahuja, C.S. and Naik, P.K., 2006. Effect of supplementation of whole linted cottonseed on the performance and blood biochemical profile of crossbred cows in summer. Animal Nutrition and Feed Technology, 6, 95–102Google Scholar
  20. Shimada, N., Murata, H. & Miyazaki, S., 2008. Safety evaluation of Bt plants. The Japan Agricultural Research Quarterly, 42, 251–259Google Scholar
  21. Singh, M. and Ludhri, R.S., 2001. Somatic cell counts in Murrah buffaloes (Bubalus bubalis) during different stages of lactation, parity and season. Asian–Australasian Journal of Animal Sciences, 14, 189–192Google Scholar
  22. Singh, M., Tiwari, D.P., Kumar, A., and Ravi Kumar, M., 2003. Effect of feeding transgenic cottonseed vis-à-vis non-transgenic cottonseed on haemato-biochemical constituents in lactating Murrah buffaloes. Asian–Australasian Journal of Animal Sciences, 16, 1732–1737Google Scholar
  23. Singhal, K.K., Kumar, R.S., Tyagi, A.K. and Rajput, Y.S., 2006. Evaluation of Bt cottonseed as protein supplement in the ration of lactating dairy cows. Indian Journal of Animal Sciences, 76, 532–537Google Scholar
  24. Smith, F.H., 1968. Estimation of free gossypol in cottonseed meal and cottonseed meats: modified method. Journal of American Oil Chemical Society, 45, 903CrossRefGoogle Scholar
  25. Stein, H.H., Rice, D.W., Smith, B.L., Hinds, M.A., Sauber, T.E., Pedersen, C., Wulf, D.M. and Peters, D.N., 2009. Evaluation of corn grain with the genetically modified input trait DAS-59122-7 fed to growing-finishing pigs. Journal of Animal Science, 87, 1254–1260CrossRefPubMedGoogle Scholar
  26. SYSTAT, 1997. Statistical package for social sciences Version 7.0. SPSS Inc., Chicago, IL, USA.Google Scholar
  27. Talapatra, S.K., Roy, S.C., and Sen, K.C., 1940. The analysis of mineral constituents in biological materials: Estimation of phosphorus, chlorine, calcium, manganese, sodium and potassium in foodstuffs. Indian Journal of Veterinary Science and Animal Husbandry, 10, 243–258Google Scholar
  28. Van Keulen, J. and Young, B.A., 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestion studies. Journal of Animal Science, 44, 282–287Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ranjan K. Mohanta
    • 1
  • Kamal K. Singhal
    • 1
  • Amrish K. Tyagi
    • 1
  • Y. S. Rajput
    • 2
  • Shiv Prasad
    • 3
  1. 1.Dairy Cattle Nutrition DivisionNational Dairy Research InstituteKarnalIndia
  2. 2.Animal Biochemistry DivisionNational Dairy Research InstituteKarnalIndia
  3. 3.Dairy Cattle Breeding DivisionNational Dairy Research InstituteKarnalIndia

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