Tropical Animal Health and Production

, Volume 41, Issue 7, pp 1213–1217 | Cite as

Changes in colostrum of Murrah buffaloes after calving

  • A. K. Dang
  • Suman Kapila
  • Maneesh Purohit
  • Charan Singh
Original Paper

Abstract

Colostrum samples were collected from 8 Murrah buffaloes on days 1, 2, 3, 4 and 5 after calving. Levels of IgG averaged 54.0 mg/ml at calving, then decreased significantly (P < 0.01). IgA and IgM on day 1 were 3.22 mg/ml and 5.22 mg/ml, respectively; both decreased during the first five days after calving. Values of IgA and IgM were higher than those reported in cows. SCC values, which were high at calving (500 000 per ml), reduced significantly (P < 0.01) on day 2, then decreased slightly until day 5 (180 000 per ml). At calving, macrophages were the most prominent cells in buffalo colostrum, followed by lymphocytes and neutrophils. Phagocytic activity was 23% at calving and reduced significantly (P < 0.01) to 14% on day 5. Phagocytic index was highest in the first colostrum, and then decreased non-significantly.

Keywords

Buffalo Colostrum Immunoglobulin SCC DLC Phagocytic activity 

References

  1. Barkema, H.W., Deluyeker, H.A., Schukken, Y.H., Lam., T.J.G.M., 1999. Quarter-milk Somatic cell count at calving and at the first six milking after calving. Preventive Veterinary Medicine 38, 1–9. doi: 10.1016/S0167-5877(98)00142-1 CrossRefPubMedGoogle Scholar
  2. Butler, J.E., 1973. The occurrence of immunoglobulin fragments, two types of lactoferrin IgG2 complex in bovine colostral and milk whey. Biochim. Biophys. Acta 275, 341.Google Scholar
  3. Dang, A.K., Kapila, S., Tomar, P., Singh, C., 2007a. Immunity of the buffalo mammary gland during different physiological stages. Asian Australasian Journal of Animal Sciences 20, 1174–1181.Google Scholar
  4. Dang, A.K., Kapila, S., Tomar, P., Singh, C., 2007b. Relationship of blood and milk cell counts and mastitic pathogens in Murrah buffaloes. Italian Journal of Animal Science 6, 821–824.Google Scholar
  5. Dang, A.K., Kapila, S., Singh, C., Sehgal, J.P., 2008. Milk differential cell counts and compositional changes in cows during different physiological stages. Milchwissenschaft 63, 239–242.Google Scholar
  6. Dosogne, H., Vangroenweghe, F., Barrio, B., Rainard, P., Burvenich. C., 2001. Decreased number and bactericidal activity against Staphylococcus aureus of the resident cells in milk of dairy cows during early lactation. Journal of Dairy Research 68, 539–549. doi: 10.1017/S0022029901005088 CrossRefPubMedGoogle Scholar
  7. Georgiev, P., 2008. Difference in chemical composition between cow colostrums and milk. Bulgarian Journal of Veterinary Medicine 11, 3–12.Google Scholar
  8. Ginel, J. Margarito, J. Molleda, R. López, M. Novales, W. Bernadina., 2003. Biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of canine serum IgA, IgG and IgM. Research in Veterinary Science 68, 107 – 110. doi: 10.1016/S0034-5288(96)90002-8
  9. Guidry, A.J., Paape, M.J.,Pearson, R. E., 1976. Effects of parturition and lactation on blood and milk cell concentrations, corticosteroids, and neutrophil phagocytosis in the cow. American Journal of Veterinary Research 37, 1195–1200.PubMedGoogle Scholar
  10. Jensen, D.L., Eberhart, R.J., 1981. Total and differential cell counts in secretions of the nonlactating bovine mammary gland. American Journal of Veterinary Research 42, 743–747.PubMedGoogle Scholar
  11. Larson, B.L., Heary, H.L. Jr., Devery, J.E., 1980. Immunoglobulin production and transport by the mammary gland. Journal of Dairy Science 63, 665–671.PubMedGoogle Scholar
  12. Meglia, G.E. Johannisson, A. Agenäs.S., Holtenius, K. Waller, K.P., 2001. Effects of feeding intensity during the dry period on leukocyte and lymphocyte sub-populations, neutrophil function and health in periparturient dairy cows. http://www.medscape.com/ medline/publication.
  13. McEwan, A. D., Fisher, E. W., Selman, I. E., Penhale, W. J., 1970. A turbidity test for the estimation of immune globulin levels in neonatal calf serum. Clinica Chimica Acta 27, 155–163. doi: 10.1016/0009-8981(70)90390-6 CrossRefGoogle Scholar
  14. Mehrzad, J., Dosogne, H., Meyer, E., Burvenich, C., 2001. Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows. Veterinary Research 32, 131–144. doi: 10.1051/vetres:2001100 CrossRefPubMedGoogle Scholar
  15. Quigley, J.D., Martin, K.R., Dowlen, H.H., Wallis, L.B., Lamar, K., 1994. Immunoglobulin concentration, specific gravity and nitrogen fractions of colostrums in Jersey cows. Journal of Dairy Science 77, 264–269.PubMedCrossRefGoogle Scholar
  16. Singh, A., Ahuja, P. and Singh, B., 1993. Individual variation in the composition of colostrums and absorption of colostral antibodies by the precolostral buffalo calf. Journal of Dairy Science 76, 1148–1156.PubMedCrossRefGoogle Scholar
  17. Sugisawa, H., Itou, T., Sakai, T., 2001. Promoting effect of colostrum on the phagocytic activity of bovine Poly morpho nuclear leucocytes in vitro. Biology of Neonate 79, 140–144. doi: 10.1159/000047080 CrossRefGoogle Scholar
  18. Zhang, H. Guo, J., Li-min, L., 2001. Determination of IgG in bovine colostrums. China Dairy Industry.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • A. K. Dang
    • 1
  • Suman Kapila
    • 1
  • Maneesh Purohit
    • 1
  • Charan Singh
    • 1
  1. 1.National Dairy Research InstituteKarnalIndia

Personalised recommendations