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Tropical Animal Health and Production

, Volume 41, Issue 7, pp 1115–1126 | Cite as

An in vitro nutritive evaluation and rumen fermentation kinetics of Sesbania aculeate as affected by harvest time and cutting regimen

  • M. R. Al-MasriEmail author
Original Paper

Abstract

The nutritive value of Sesbania aculeate harvested after 60 and 120 days of planting and subjected to two cutting regimen (15 or 30 cm length) was evaluated by determination of the crude protein (CP), crude fibre (CF), buffer soluble nitrogen (BS-N), buffer soluble non-protein nitrogen (BS-NPN) and cell wall constituents (neutral-detergent fibre; NDF, acid-detergent fibre; ADF and lignin). In vitro digestible organic matter (IVDOM), metabolizable energy (ME), microbial nitrogen (MN) and biomass (MBM) production were also estimated in the experimental plant samples after their incubation with rumen fluid for 96 h in the absence or presence of polyethylene glycol (PEG, 6000) at a ratio of 2:1 PEG:substrate. Fermentation characteristics (initial gas production; a, gas production during incubation; b, potential gas production; a+b, fractional rate of gas production; c) were assessed using an in vitro incubation technique with rumen fluid. There was a significant (P < 0.05) effect of harvest time on all studied nutritive parameters and fermentation characteristics. The early harvest plant samples (after 60 days of planting) gave significantly higher values of IVOMD, ME, CP, BS-N, BS-NPN, MN, MBM and fractional rate of gas production and lower values of CF and cell wall constituents than the late harvest. Crude protein, BS-N, BS-NBN, IVOMD and ME were negatively correlated with CF and cell wall constituents. Metabolisable energy and IVOMD were positively correlated with CP, BS-N and BS-NPN. Cutting treatments significantly affected the CP, CF, BS-N, BS-NPN, NDF, ADF, IVDOM, ME, potential gas production and b values. There was no significant (P > 0.05) effect of added PEG on IVDOM, ME, MN, MBM, fermentation characteristics and gas production over 96 h. The greatest proportion of gas production occurred between 6 and 24 h of incubation. The fractional rate of gas production from 100 mg substrate was higher (0.046 mL/h) for the plant samples harvested at early stage and cut at 30 cm length than harvested at late stage (0.018 mL/h). C values were negatively correlated with lignin concentrations. The amount of MN and MBM produced from 100 mg substrate amounted to 1.29 mg and 14.95 mg at early maturity stage and 0.68 mg and 7.89 mg at late stage, respectively. Microbial nitrogen and MBM production were negatively correlated with CF, cell wall constituents and gas production but positively correlated with CP, BS-N and BS-NPN.

Keywords

Gas production Fermentation Microbial mass Nutrient Protein Energy Harvesting Cutting 

Abbreviations

a

initial gas production

a+b

potential gas production

ADF

acid-detergent fibre

b

gas production during incubation

BS-N

buffer soluble nitrogen

BS-NBN

buffer soluble non-protein nitrogen

c

fractional rate of gas production

CF

crude fibre

CP

crude protein

CT

condensed tannins

DM

dry matter

HT

hydrolysable tannins

IVDOM

in vitro digestible organic matter

L

lignin

MBM

microbial biomass

ME

metabolizable energy

MN

microbial nitrogen

NDF

neutral-detergent fibre

r

correlation coefficient

TP

total phenols

Y

predicted daily intake

Notes

Acknowledgments

The author thank the Director General and Head of Agriculture Department, Atomic Energy Commission of Syria, for their encouragement and financial support.

References

  1. Al-Masri, M.R., 2003. An in vitro evaluation of some unconventional ruminant feeds in terms of the organic matter digestibility, energy and microbial biomass. Tropical Animal Health and Production, 35, 155–167. doi: 10.1023/A:1022877603010 CrossRefPubMedGoogle Scholar
  2. Al-Masri, M.R., 2006. Nutritive value of some indigenous range plants and their in vitro biochemical fermentable characteristics. Report on Scientific Research Final A/FRSR 361, (Department of Agriculture, Atomic Energy Commission, Syria)Google Scholar
  3. Al-Masri, M.R., 2007. An in vitro evaluation of some drought-tolerent native range plants in terms of ruminal microbial nitrogen, microbial biomass and their fermentation characteristics utilizing a gas-production technique. Tropical Grasslands, 41, 292–300Google Scholar
  4. Al-Masri, M.R. and Mardini, M., 2007. Effect of harvest time and sampling site on the nutritive value of Sesbania aculeate and Kochia indica. Report on Scientific Laboratory Study AECS-A/RSS 710, (Department of Agriculture, Atomic Energy Commission, Syria)Google Scholar
  5. Al-Masri, M.R., Zarkawi, M. and Khalifa, K., 2007. Partial substation of Atriplex lentiformis for wheat straw in the diet of Damascus does. Tropical Grasslands, 41, 301–307Google Scholar
  6. AOAC, 1990. Official methods of analysis. 15th ed, (Association of Official Analytical Chemist, Virginia, USA)Google Scholar
  7. Blümmel, M., Cone, J.W., Van Gelder, A.H., Nshalai, I., Umunna, N.N., Makkar, H.P.S. and Becker, L., 2005. Prediction of forage intake using in vitro gas production methods: Comparison of multiphase fermentation kinetics measured in an automated gas test, and combined gas volume and substrate degradability measurements in a manual syringe system. Animal Feed Science and Technology, 123–124, 517–526. doi: 10.1016/j.anifeedsci.2005.04.040
  8. Chumpawadee, S. and Pimpa, O., 2008. Effect of non forage high fibrous feedstuffs as fiber sources in total mixed ration on gas production characteristics and in vitro fermentation. Pakistan Journal of Nutrition, 7, 459–464CrossRefGoogle Scholar
  9. Cone, J.W. and Van Gelder, A.H., 2000. In vitro microbial protein synthesis in rumen fluid estimated with the gas production technique. In: Gas Production: Fermentation Kinetics for Feed Evaluation to Assess Microbial Activity, (British Society of Animal Science, Penicuik, UK), 25–26Google Scholar
  10. Czerkawski, J.W., 1986. An Introduction to Rumen Studies, (Pergamon Press, Oxford)Google Scholar
  11. Gasmi-Boubaker, A., Kayouli, C. and Buldgen, A., 2005. In vitro gas production and its relationship to in situ disappearance and chemical composition of some Mediterranean browse species. Animal Feed Science and Technology, 123–124, 303–311Google Scholar
  12. George, M.R. and Bell, M.E., 2001. Using stage of maturity to predict the quality of annual range forage, Rangeland Management Series, Publication 8019, (Division of Agriculture and Natural Resources, University of California, USA)Google Scholar
  13. Getachew, G., Makkar, H.P.S. and Becker, K., 2000. Effect of polyethylene glycol on in vitro degradability of nitrogen and microbial protein synthesis from tannin-rich browse and herbaceous legumes. British Journal of Nutrition, 84, 73–83PubMedGoogle Scholar
  14. Getachew, G., Makkar, H.P.S. and Becker, K., 2002. Tropical browsws: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. Journal Agricultural Science (Cambridge), 139, 341–352Google Scholar
  15. Getachew, G., DePeters, E.J., Robinson, P.H. and Fadel, J.G., 2005. Use of an in vitro rumen gas production technique to evaluate microbial fermentation of ruminant feeds and its impact on fermentation products. Animal Feed Science and Technology, 123–124, 547–559Google Scholar
  16. Goel, G., Makkar, H.P.S. and Becker, K., 2008. Changes in microbial community structure, methanogenesis and rumen fermentation in response to saponin-rich fractions from different plant materials. Journal of Applied Microbiology, 105, 770–777CrossRefPubMedGoogle Scholar
  17. Hardarson, G. and Danso, S.K.A., 1990. Use of 15N Methodology to Assess Biological Nitrogen Fixation. In: Use of Nuclear Technique in Studies of Soil-Plant Relationships, Training Course Series No. 2, (International Atomic Energy Agency, Vienna, Austria), 129–160Google Scholar
  18. Hossain, M.A., Muetzel, S. and Becker, K., 2001. Nutritional value and antinutritional factors in leaves from different Sesbania species. Tropical Science, 41, 126–132Google Scholar
  19. Jung, H.G., Mertens, D.R. and Payne, A.J., 1997. Correlation of acid detergent lignin and Klason lignin with digestibility of forage dry matter and neutral detergent fibre. Journal Dairy Science, 80, 1622–1628CrossRefGoogle Scholar
  20. Khazaal, K., Dentinho, J.M., Ribeiro, J.M. and Ørskov, E.R., 1995. Prediction of apparent digestibility and voluntary intake of hays fed to sheep: comparison between using fibre components, in vitro digestibility or characteristics of gas production or nylon bag degradation. Animal Science, 61, 527–538Google Scholar
  21. Khazaal, K., Parissi, Z., Tsiouvaras, C., Nastis, A. and Ørskov, E.R., 1996. Assessment of phenolics-related anti-nutritive levels using the in vitro gas production technique: A comparison between different types of polyvinylpolypyrrolidone or polyethylene glycol. Journal of Food Science and Agriculture, 71, 405–414CrossRefGoogle Scholar
  22. Kumar, R. and Vaithiyanathan, S., 1990. Occurrence, nutritional significance and effect on animal productivity of tannins in tree leaves. Animal Feed Science and Technology, 30, 21–38CrossRefGoogle Scholar
  23. Kurdali, F., 2004. Estimates of dry matter yield and nitrogen uptake in sorghum grown on saline and non-saline soils manured with Dhaincha plant tissues. Journal of Plant Nutrition, 27, 1611–1633CrossRefGoogle Scholar
  24. Makkar, H.P.S. and Becker, K., 1996. Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Animal Feed Science and Technology, 63, 211–228CrossRefGoogle Scholar
  25. Makkar, H.P.S., Blümmel, M. and Becker, K., 1995. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques. British Journal of Nutrition, 73, 897–913CrossRefPubMedGoogle Scholar
  26. Mandal, J.K., Singh, G., Victor, U.S. and Sharma, K.L., 2003. Green manuring: its effect on soil properties and crop growth under rice-wheat cropping system. European Journal of Agronomy, 19, 225–237CrossRefGoogle Scholar
  27. McSweeny, C.S., Palmer, B., McNeill, D.M. and Krause, D.O., 2001. Microbial interactions with tannins: nutritional consequences for ruminants. Animal Feed Science and Technology, 91, 83–93CrossRefGoogle Scholar
  28. Mekoya, A.K., 2008. Multipurpose fodder trees in Ethiopia: farmers’ perception, constraints to adoption and effect of long-term supplementation on sheep performance, (PhD thesis, University of Wageningen)Google Scholar
  29. Melagu, S., Peters, K.P., Tegegne, A., 2003. In vitro and in situ evaluation of selected multipurpose trees, wheat bran and Lablab purpureus as potential feed supplements of tef (Eragrostis tef) straw. Animal Feed Science and Technology, 108, 159–179CrossRefGoogle Scholar
  30. Menke, K.H. and Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7–55Google Scholar
  31. Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, Cambridge, 93, 217–222CrossRefGoogle Scholar
  32. Naumann, C. and Bassler, R., 1976. Die chemische Untersuchung von Futtermitteln, Methodenbuch Band III, (Neumann-Neudamm, Berlin)Google Scholar
  33. Ortiz-Tovar, M. G., López-Miranda, J., Cerrillo-Soto, M.A., Juárez-Reyes, A., Favela-Torres, E. and Soto-Cruz, N., 2007. Effect of solid substrate fermentation on the nutritional quality of agro-industrial residues. Interciencia, 32, 339–343.Google Scholar
  34. Ørskov, E.R. and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, Cambridge, 92, 499–503.CrossRefGoogle Scholar
  35. Rodrigues, M.A.M., Guedes, C.M., Cone, J.W., van Gelder, A.H., Ferreira, L.M.M. and Sequeira, C.A., 2007. Effects of phenolic acid structures on meadow hay digestibility. Animal Feed Science and Technology, 136, 297–311.CrossRefGoogle Scholar
  36. Sallam, S.M.A., Buenob, I.C.S., Godoyb, P.B., Nozellab, E.F., Vittib, D.M.S.S. and Abdallab, A.L., 2008. Nutritive value assessment of artichoke (Cynara scolymus) by-products as an alternative feed resource for ruminants. Tropical and Subtropical Agroecosystems, 8, 181–189Google Scholar
  37. Sileshi, G., Sithanatham, S., Mafongoya, P.L., Ogol, C.K. and Rao, M.R., 2003. Biology of Mesoplatys ochropetra Stal (Coleopetra: Chrysomelidae), a pest of Sesbania species, in southern central Africa. African Entomology, 11, 49–58Google Scholar
  38. Steingass, H. and Menke, K., 1986. Schatzung des energetischen Futterwertes aus der in vitro mit Pansensaft bestimmten Gasbildung und der chemischen Analyse. 1. Untersuchungen zur Methode. Tierernährung, 14, 251–270Google Scholar
  39. Van Soest, P.J., Robertson, J.B. and Leis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597PubMedCrossRefGoogle Scholar
  40. Waghorn, G.C., Jones, W.T., Shelton, I.D. and McNabb, W.C., 1990. Condensed tannins and the nutritive value of herbage. Proceeding New Zealand Grasslands Associates, 51, 171–176Google Scholar
  41. Yaduvanshi, N.P.S., 2003. Substitution of inorganic fertilizers by organic manures and the effects on soil fertility in a rice-wheat rotation on reclaimed sodic soil. Indian Journal of Animal Science, 140, 161–168Google Scholar
  42. Zarkawi, M., Al-Masri, M.R. and Khalifa, K., 2003. An observation on yield and nutritive value of Sesbania aculeate and its feeding to Damascus does. Tropical Grasslands, 37, 187–192Google Scholar
  43. Zarkawi, M., Al-Masri, M.R. and Khalifa, K., 2005. Nutritive value of Sesbania aculeate grown on salty soil and its effect on reproductive parameters of Syrian Awassi ewes. Australian Journal of Agricultural Research, 56, 819–825CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Agriculture, Atomic Energy CommissionDamascusSyria

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