Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen
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Classical swine fever (CSF) is a highly contagious and severe viral disease of swine resulting in substantial production losses in different farming systems in many regions of the world. The accurate and rapid detection of CSF outbreaks is reliant on sensitive and specific laboratory testing and is a key component of disease control. Specific detection of CSF virus can be achieved by virus isolation in tissue culture, antigen capture or the detection of viral RNA using molecular techniques. In order to reduce the time taken to achieve a diagnostic result and simplify testing methods, an antigen capture ELISA using immunomagnetic beads (IMB) as the solid phase was developed and compared to a microplate-based antigen capture (AC)-ELISA. The IMB-ELISA has up to 64-fold greater analytical sensitivity than the AC-ELISA and initial estimates of diagnostic sensitivity and specificity are 100%. The IMB-ELISA has a highly robust, rapid and stable test format and is simpler to perform than the AC-ELISA. The IMB-ELISA has the added advantage that a result can be sensitively and specifically determined by eye, lending it to the possibility of adaptation to a near-to-field test with minimal equipment or expertise needed.
KeywordsClassical swine fever virus Diagnosis Rapid test Immunomagnetic Bead Microparticle ELISA
This work was supported by the Australian Centre for International Agricultural Research (ACIAR), the Department of Livestock and Fisheries of Lao PDR and CSIRO Division of Livestock Industries. The authors wish to acknowledge Ms. Manivanh Phruaravanh, Miss Vilaywan Soukvilai and Mr. Lapinh Phithakhep for technical and logistical support.
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