Tropical Animal Health and Production

, Volume 41, Issue 5, pp 807–818 | Cite as

Recovery from adverse effects of heat stress on slow-growing chicks in the tropics 1: Effect of ascorbic acid and different levels of betaine

  • Y. A. AttiaEmail author
  • R. A. Hassan
  • E. M. A. Qota
Original Paper


Three hundreds, 21 d-old slow-growing chicks were randomly divided among 5 treatments, of 5 replicates each. Each replicate contained 12 unsexed chicks housed in (1 × 1) a floor pen. A group was kept under thermoneutral condition at 28 ± 4°C and RH was 55 ± 3% during 21–84 d of age (positive control) and fed corn-soybean meal diet. The other four groups were kept for three successive days per week under heat stress (HS) at 38 ± 1.4°C and 49 ± 2% RH from 12.00 to 16.00 pm. Chicks in HS treatments were fed corn-soybean meal diet without (negative control) or with 250 mg AA/kg diet and Bet at 0.5 and 1 g/kg diet. HS decreased productive performance, increased (P < 0.05) meat dry matter, plasma triglyceride and serum calcium whereas decreased (P > 0.05) plasma glucose, serum total protein and water holding capacity (WHC) of meat. AA and 1 g of Bet/kg diet was equally potent for partial relief (P < 0.05) of the negative effect of HS on growth, increased (P < 0.05) feed intake, protein digestibility (P < 0.05), dressing out percentage, liver and giblets, whilst improved (P < 0.05) feed conversion ratio (FCR). Also, a complete recovery from the negative effect (P < 0.05) of HS shown on plasma glucose and partial recovery (P < 0.05) observed in total protein, triglyceride, blood pH, packed cell volume (PCV), hemoglobin (Hgb), rectal temperature (RT) and respiration rate (RR) and improved humoral immune competence to sheep red blood cell (SBRCs) test.


Ascorbic acid Betaine Heat stress Productive and physiological traits 



ascorbic acid


body weight gain






crude ash


crude protein


crude fibre




dry matter


ether extract


feed conversion ratio






heat stress


least significant difference


National Research Council


organic matter


hydrogen power


probability level


rectal temperature


respiration rate


sulphur amino acid


standard deviation


sheep red blood cells




water holding capacity


  1. Al-Ghamdi, Zahraa, H., 2008. Effects of Commutative heat stress on immunoresponses in broiler chickens reared in closed system. International Journal of Poultry Science 7, 64–968.Google Scholar
  2. AOAC. 1995. Official methods of Analysis, 15th edn, Association of Official Analytical Chemists Washington, DC, USA.Google Scholar
  3. Armstrong, W. D. and Carr, C. W. 1964. Physiological Chemistry Laboratory Direction, 3rd Bursus Publishing Co. Minneapolis, Minnesota, USA.Google Scholar
  4. Attia, Y. A., 2003. Performance, carcass characteristics, meat quality and plasma constituents of meat type drakes fed diets containing different levels of lysine with or without a microbial phytase. Archiv of Animal Nutrition 66, 39–48. doi: 10.1080/0003942031000086635 CrossRefGoogle Scholar
  5. Attia, Y. A., Hassan, R. A., Shehatta, M. H. and Abd El-Hady, S. B., 2005. Growth, carcass quality and blood serum constituents of slow growth chicks as affected by betaine additions to diets containing 2. Different levels of methionine, International Journal of Poultry Science, 11, 856–865.Google Scholar
  6. Attia, Y. A., Bohmer, Barbara M. and Roth-Maier, Dora A., 2006. Responses of broiler chicks raised under constant relatively high ambient temperature to enzymes, amino acid supplementations, or diet density, Archiv Für Geflügelkunde, 70, 80–91.Google Scholar
  7. Chendrimada, T. P., Neto, M. G., Pesti, G. M., Davis A. J. and Bakalli, R. H. I., 2002. Determination of the betaine content of feed ingredients using high-performance liquid chromatography, Journal of the Science of Food and Agriculture, 82, 1556–1563. doi: 10.1002/jsfa.1214 CrossRefGoogle Scholar
  8. Daghir, N. J., 2008. Poultry production in hot climates, 2nd edn. Ed. N. J. Daghir, CAB International.Google Scholar
  9. Eilers, R. J., 1967. Notification of final adoption of an international method and standard solution for hemoglobinometry specifications for preparation of a standard solution. American Journal of Clinical Pathology, 47: 212–214.PubMedGoogle Scholar
  10. Esteve-Garcia, E. and Mack, S., 2000. The effect of DL-methionine and betaine on growth performance and carcass characteristics in broilers, Animal Feed Science and Technology, 87, 85–93. doi: 10.1016/S0377-8401(00)00174-7 CrossRefGoogle Scholar
  11. Garcia N. M.; Pesti, G. M. and Bakalli, R. I., 2000. Influence of dietary protein level on the broiler chickens response to methionine and betaine supplements, Poultry Science, 79, 1478–1484.Google Scholar
  12. Gous, R. M. and Morriss, T.R., 2005. Nutritional interventions in alleviating the effects of high temperatures in broiler Production, World’s Poultry Science Journal, 61, 463–475. doi: 10.1079/WPS200568 CrossRefGoogle Scholar
  13. Graham, H., 2002. Betaine-Combating heat stress in poultry, Afma Matrix, December, 15, 16–17.Google Scholar
  14. Hassan, R.A., Attia, Y. A. and El-Ganzory, E. H., 2005. Growth, carcass quality, and blood serum constituents of slow growth chicks as affected by betaine additions to diets containing 1. Different levels of choline, International Journal of Poultry Science, 4, 840–850.CrossRefGoogle Scholar
  15. Kai, O.H., Nagase, N., Ishikawa, M., Suzuki, K. and Sato, K., 1988. Effects propylthiouracial PTU on the immunological status of the chickens, Development comparative Immunology, 12, 145. doi: 10.1016/0145-305X(88)90032-8 CrossRefGoogle Scholar
  16. Kettunen, H., Tiihonen, K., Peuranen, S., Saarinen, M.T. and Remus, J. C., 2001. Dietary betaine accumulates in the liver and intestinal tissue and stabilizes the intestinal epithelial structure in healthy and coccidia-infected broiler chicks, Comparative Biochemistry and Physiology, A 130, 759–769. doi: 10.1016/S1095-6433(01)00410-X Google Scholar
  17. Kutlu, H. R., 2001. Influences of wet feeding and supplementation with ascorbic acid on performance and carcass composition of broiler chicks exposed to a high ambient temperature, Archive Für Tierernahrung, 55: 127–139.Google Scholar
  18. Lin, H., Du, R. and Zhang, Z. Y., 2000. The peroxidation in tissues of heat-stressed broilers, Asian-Australian Journal of Animal Science, 13, 1373–1376.Google Scholar
  19. Lin, H., Buyse, J., Sheng, Q. K., Xie, Y. M. and Song, J. L., 2003. Effects of ascorbic supplementation on the immune function and laying performance of heat-stressed laying hens, Journal Feed Agriculture and Environment, 1, 103–107.Google Scholar
  20. Lin, H., Jiao, H. C., Buyse, J. and Decuypere, E., 2006. Strategies for preventing heat stress in poultry, World’s Poultry Science Journal, 62, 71–85. doi: 10.1079/WPS200585 CrossRefGoogle Scholar
  21. Mahmoud, K. Z., Edens, E. W., Elsen, E. J. and Havenstein, G. B., 2004. Ascorbic acid decreases heat shock protein 70 and plasma corticosterone response in broilers Gallus gallus domesticus subjected to cyclic heat stress, Comparative Biochemical Physiology, B 137, 35–42. doi: 10.1016/j.cbpc.2003.09.013 Google Scholar
  22. Mashaly, M. M., Hendricks, G. L., Kalama, M. A., Gehad, A. E., Abbas, A. O. and Patterson, P. H., 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens, Poultry Science, 83, 889–894.PubMedGoogle Scholar
  23. Mujahid, A., Yoshik, Y., Akiba, Y. and Toyomizu, M., 2005. Superoxide radical production in chicken skeletal muscle induced by acute heat stress, Poultry Science, 84, 307–314.PubMedGoogle Scholar
  24. National Research Cauncil NRC, 1994. Nutrient Requirements of Poultry, 9th revised Edition, National Academy Press. Washington DC., USA.Google Scholar
  25. Pardue, S. I., Thaxton, J. P. and Brake, J. T., 1985. Influence of supplemental ascorbic acid on broiler performance following exposure to high temperature, Poultry Science, 64, 1334–1338.PubMedGoogle Scholar
  26. Puthpongsiriporn, U., Scheideler, S. E., Sell, J. L. and Beck, M. M., 2001. Effects of various E and C supplementation on performance, in Vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress, Poultry Science, 80, 1190–1200.PubMedGoogle Scholar
  27. Remus, J. C., Pierson, E. E. M. and Hruby, M., 2004. The evaluation of betaine and enzymes in coccidian challenged broilers, XXII Poultry Congress, Istanbul, Turkey 8–13 June, 2004.Google Scholar
  28. Sahin, K., Sahin, N., Onderci, I., Gursu, M. F. and Cikim, G., 2002. Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities and serum metabolites of broiler chickens, Biological Trace Elements Research, 89, 53–64. doi: 10.1385/BTER:89:1:53 CrossRefGoogle Scholar
  29. Sahin, K., Sahin, N., Onderci, I., Gursu, M. F. and Kucuk, G., 2003. Dietary vitamin C and folic acid supplementation ameliorates the detrimental effects of heat stress in Japanese quail, Journal of Nutrition, 133, 1882–1886.PubMedGoogle Scholar
  30. SAS Institute 1990 SAS-User’s Guide, Statistics, Version 6, 4th edn. SAS Institute Inc., Cary, NC., USA.Google Scholar
  31. Swain, B. K. and Johri, T. S., 2000. Effect of supplemental methionine, betaine and their combinations on the performance and immune response of broilers, British Poultry Science, 41, 83–88. doi: 10.1080/00071660086457 PubMedCrossRefGoogle Scholar
  32. Trinder, P., 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor, Animal Clinical Biochemistry, 6, 24.Google Scholar
  33. Türker, M., Alp, M. and Kocabacli, N., 2004. Performance of broiler chicks fed on reduced methionine diets supplemented with betaine, XXII Poultry Congress, Istanbul, Turkey 8–13 June, 2004.Google Scholar
  34. Virtanen, E. and Rosi, L., 1995. Effects of betaine on methionine requirement of broilers under various environmental condition, pp. 88–92 in proceeding of the Australian Poultry Science, Symposium, Sydney, Australia.Google Scholar
  35. Waldroup, P.W. and Fritts, C. A., 2005. Evaluation of separate and combined effects of choline and betaine in diets for male broilers, International Journal of Poultry Science, 4, 442–448.CrossRefGoogle Scholar
  36. Wang, Y. Z., Xu, Z. R. and Feng, G., 2004. The effect of betaine and DL-methionine on growth performance and carcass characteristics in meat ducks, Animal Feed Science and Technology, 116, 151–159. doi: 10.1016/j.anifeedsci.2004.05.003 CrossRefGoogle Scholar
  37. Whitehead, C. C. and Keller, T., 2003. An update on ascorbic acid in poultry, World’s Poultry Science Journal, 59, 161–184. doi: 10.1079/WPS20030010 CrossRefGoogle Scholar
  38. Yahav, S., Shinder, D., Tanny, J. and Cohen, S., 2005. Sensible heat loss, the broiler’s paradox, World’s Poultry Science Journal, 61,463–475. doi: 10.1079/WPS200568 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour BranchAlexandria UniversityDamanhourEgypt
  2. 2.Department of Poultry Nutrition, Animal production Research InstituteAgriculture Research Center, Ministry of Agriculture and Land reclamationDamanhourEgypt

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