Tropical Animal Health and Production

, Volume 41, Issue 4, pp 427–430 | Cite as

Post-vaccination antibodies profile against Peste des petits ruminants (PPR) virus in sheep and goats of Punjab, Pakistan

  • H. A. Khan
  • M. Siddique
  • M. Arshad
  • M. Abubakar
  • M. Akhtar
  • M. J. Arshad
  • M. Ashraf
Short Communication

Abstract

A total of 70 sheep and 330 goats were selected randomly. All the animals were kept under same housing and management conditions. Serum samples were collected from all the animals and tested for the presence of antibodies against Peste des petits ruminants (PPR) virus using competitive ELISA (cELISA). All the animals were found negative showing percentage inhibition (PI) values <50. The animals were vaccinated against PPR with Nig/75/1 strain vaccine of PPR Serum samples were collected from randomly selected 12 sheep and 30 goats at 10, 30 and 45 days post-vaccination. The samples were subjected to cELISA to determine the presence of antibodies against PPRV. The samples with PI >50 were considered as sero-positive. The sheep found positive at 10, 30 and 45 days post-vaccination were 1(8.3%), 7(58.3%) and 12(100%) respectively. In case of goats 3(10.0%), 29(96.6%) and 27(90.0%) animals gave positive results at 10, 30 and 45 days post-vaccination respectively. Mean PI values in sheep at 10, 30 and 45 days post-vaccination were recorded as 37, 65 and 91 respectively, whereas in goats these values were 43, 78 and 86 respectively.

Keywords

Peste des petits ruminants Post vaccination Antibody titre cELISA Sheep and goats, Pakistan 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • H. A. Khan
    • 1
  • M. Siddique
    • 1
  • M. Arshad
    • 1
  • M. Abubakar
    • 2
  • M. Akhtar
    • 3
  • M. J. Arshad
    • 2
  • M. Ashraf
    • 1
  1. 1.Department of MicrobiologyUniversity of AgricultureFaisalabadPakistan
  2. 2.National Veterinary LaboratoryIslamabadPakistan
  3. 3.Department of ParasitologyUniversity of AgricultureFaisalabadPakistan

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