Tropical Animal Health and Production

, Volume 41, Issue 4, pp 493–498 | Cite as

Effect of bromelain on milk yield, milk composition and mammary health in dairy goats

  • A. Contreras
  • M. J. Paape
  • R. H. Miller
  • J. C. Corrales
  • C. Luengo
  • A. Sánchez
Original Paper
First Online:
Received:
Accepted:

Abstract

A 7 month prospective cohort study was designed to determine if feeding bromelain to dairy goats influenced the MSCC, milk yield, milk composition and the incidence of IMI. Forty-four clinically normal goats from 2nd to 6th parities were studied. Daily bromelain dosage was 7.4 grams/animal (185-mg/Kg weight). Samples for diagnostic bacteriology were collected from each udder half every 2 weeks. Samples for MSCC and composition were obtained every 42 days. Milk yield was also recorded every 42 days. Bromelain affected milk protein and fat but not MSCC, milk yield or milk lactose. Bromelain did not decrease the MSCC in healthy goats. Milk protein and fat increased in the bromelain treated group (P < 0.01), which is important for dairymen because premiums are paid milk fat and protein content. No clinical mastitis was detected in the goats for the total study period and incidence rate of subclinical IMI was 5.7%. Relative risk was 1.50 (0.28 < RR < 8.12) which means that the bromelain had no significant effect on IMI (P > 0.05). In addition, the use of pineapple by-products could be especially important in tropical countries were pineapple waste seems to be a pollution problem.

Keywords

Bromelain Goat Milk composition Milk production Subclinical intrammamary infection 

Abbreviations

IMI

intramammary infection

MSCC

Milk somatic cell counts

RR

relative risk

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Notes

Acknowledgements

The first author wishes to thank the Fundación Seneca for financial help during his stay at the USDA Beltsville Research Center. We acknowledge project CAL-00-046-C3-01 for financial resources. We thank Ajinomoto Co. Inc, Tokyo, Japan for supplying the bromelain. Authors thank Dr. Carlos Fernández Martínez for analyses of the ingredients and chemical composition of diet.

References

  1. Brakebusch, M., Wintergerst, U., Petropoulou, T., Notheis, G., Husfeld, L., Belohradsky, B.H. and Adam, D., 2001 Bromelain is an accelerator of phagocytosis, respiratory burst and killing of Candida albicans by human granulocytes and monocytes. European Journal of Medical Research, 6 193–200.PubMedGoogle Scholar
  2. Chandler, D.S. and Mynott, T.L., 1998 Bromelain protects piglets from diarrhoea caused by oral challenge with K88 positive enterotoxigenic Escherichia coli. Gut, 42 196–202.CrossRefGoogle Scholar
  3. Contreras, A., Corrales, J.C., Sánchez, A. and Sierra, D., 1997 Persistence of subclinical intramammary pathogens in goats throughout lactation. Journal of Dairy Science, 80 2815–2819.PubMedGoogle Scholar
  4. Dean, A.G., Dean, J.A., Couloumbier, D., Brendel, K.A., Smith, D.C., Burton, A.H., Dicker, R.C., Sullivan, K. and Fagan, R.F., 1994 Epi Info, Version 6: A Word Processing, Database, and Statistic Program for Epidemiology on Micro-Computers. Centers for Disease Control and Prevention (CDC) Atlanta, GA. (USA).Google Scholar
  5. Hale, L.P., 2004. Proteolitic activity and immunogenicity of oral bromelain within the gastroeintestinal tract of mice. International Immunopharmacology, 42 255–264. doi: 10.1016/j.intimp.2003.12.010 PubMedCrossRefGoogle Scholar
  6. Harmon, R.J., Eberhart, R.J., Jasper, D.E., Langlois, B.E., and Wilson R.A., 1990 Microbiological procedures for the diagnosis of bovine udder infection. 3rd ed. National Mastitis Council, Inc., Arlington VAGoogle Scholar
  7. Jarrige, R., 1988. Alimentation des bovins, ovins and caprins, (Institute National de la Recherche Agronomique, INRA. Paris)Google Scholar
  8. Llorca-Lionet, H. and Meschy, F., 2000 Pinneaple waste silage utilisation by growing kids in French Polynesia. Proceedings of the 7th International Conference on Goats, Tours, France, pp 136.Google Scholar
  9. Lozt-Winter, H., 1990 On the pharmacology of bromelain: an update with special regard to animal studies on dose-dependent effects. Planta Medica 56 249-253, doi: 10.1055/s-2006-960949 CrossRefGoogle Scholar
  10. Maurer, H.R., 2001 Bromelain: biochemistry, pharmacology and medical use. Cellular and Molecular Life Science, 58 1234–1245, doi: 10.1007/PL00000936 CrossRefGoogle Scholar
  11. Mynott, T.L., Guandalini, S., Raimondi, F. and Fasano, A., 1997 Bromelain prevents secretion caused by Vibrio cholerae and Escherichia coli enterotoxins in rabbit ileum in vitro. Gastroenterology, 113 175–184, doi: 10.1016/S0016-5085(97)70093-3 PubMedCrossRefGoogle Scholar
  12. Otani, S., Ishikawa, Y., Kanno, K. and Ishizawa, N., 1989 Effect of bromelain on bovine mastitis. Journal of veterinary Medicine (Japan) 812 172–175Google Scholar
  13. Paape, M.J., Nelson, T.K., Bowman, M.E., Contreras, A. and Kobayashi, H., 2000. ¿Podrá la bromelaina reducir las células somáticas en leche de cabra como lo hace en leche de vaca? En Mamitis y Calidad de Leche, pp. 351-356. (Eds Contreras., A., Sánchez, A. and Corrales, J.C.). Diego Marin Librero.Murcia, Spain.Google Scholar
  14. Paape, M.J., Poutrel, B., Contreras, A., Marco, J.C. and Capuco, A.V., 2001 Milk somatic cells and lactation in small ruminants. Journal of Dairy Science, 84 E237–E244.CrossRefGoogle Scholar
  15. Parr, M., (Ed.) 2002. Murcia Granada Goat. En: “Animal Health and Production Compendium” (CD-ROM e Internet). CAB International, Wallingford. United Kingdom.Google Scholar
  16. Roselli, M., Britti, M.S., Le Huërou-Luron, I., Marfaing, H., Zhu, W.Y., Mengheri, E., 2007. Effect of different plant extracts and natural substances (PENS) against membrane damage induced by enterotoxigenic Escherichia coli K88 in pig intestinal cells. Toxicology in Vitro, 212:224–229. doi: 10.1016/j.tiv.2006.09.012 PubMedCrossRefGoogle Scholar
  17. SAS Institute, 1999 SAS System for Windows, Release 8.0. Statistical Analysis System Institute, Inc., Cary, NC, USAGoogle Scholar
  18. Taussig, S.J., Yokoyama, M.M., Chinen, A., Onari, K., Yamakido, M., 1975. Bromelain, a proteolytic enzyme and its clinical application. A review. Hiroshima Journal of Medical Sciences, 24: 185–193PubMedGoogle Scholar
  19. Taussig, S.J. and Batkin, S., 1988. Bromelain, the enzyme comples of pineapple (Ananas comosus) and its clinical application. An update. Journal of Ethnopharmacology, 22:191-203, doi: 10.1016/0378-8741(88)90127-4 PubMedCrossRefGoogle Scholar
  20. Zavadova, E., Desser, L. and Mohr, T., 1995. Stimulation of reactive oxygen species production and cytotoxicity in human neutrophils in vitro and after oral administration of a polyenzyme preparation. Cancer Biotherapy and Radiopharmaceuticals, 10: 147–152.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • A. Contreras
    • 1
  • M. J. Paape
    • 2
  • R. H. Miller
    • 2
  • J. C. Corrales
    • 1
  • C. Luengo
    • 1
  • A. Sánchez
    • 1
  1. 1.Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de MurciaMurciaSpain
  2. 2.Bovine Functional Genomics Laboratory, USDA-ARSBeltsvilleUSA

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