Tropical Animal Health and Production

, Volume 41, Issue 2, pp 141–144 | Cite as

Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon

  • P. H. Bayemi
  • E. C. Webb
  • M. V. Nsongka
  • H. Unger
  • H. Njakoi
Short Communication


Holstein cattle of a small scale dairy production systems were screened for Brucella abortus antibodies in 21 villages in Cameroon by ELISA. Results show a general seroprevalence of 8.4% in Holstein cattle. Of the 192 cows tested, 14 were infected giving a within-sex seroprevalence of 7.3% while 6/74 bulls were infected with a seroprevalence of 8%. There was no evidence (P=0.11) of differences in seroprevalence between age groups although animals above one year and below three years accounted for nearly half of the infected animals. 64% of infected animals were found in three locations (P=0.015): Kutaba (32%), Bamendankwe (16%) and Finge (16%). A specific control programme should be organized at these locations. Measures should be taken to ensure the eradication of the disease within the population and sound control measures adopted to avoid a further spread of the disease to larger cattle populations. Infected animals should be slaughtered systematically. All farmers should be advised to boil milk before consumption. Vaccination against Brucella abortus should be instituted and use of artificial insemination propagated. In order to ensure a productive and healthy population of Holstein cows within the dairy production scheme, regular Brucella testing should be instituted.


Brucella Cameroon ELISA Holstein cattle Small scale dairy 



World organization for animal health


enzyme-linked immunosorbent assay



This work was sponsored by the International Atomic Energy Agency (IAEA) and the Heifer International Cameroon. The authors are grateful to Mr Fonguh Emannuel, Mr Njong Vincent, Mr Apiembo for samples collection and laboratory work.


  1. Akakpo A.J., Bornarel P., 1987. Epidémiologie des brucelloses animales en Afrique tropicale:Enquête clinique, sérologique et bactériologique. Rev. Sci. Techn. Off. Int. Epiz. 6: 981–1027.Google Scholar
  2. Bornarel P., Akakpo A.J., Tuekam, 1987. Epidemiology of bovine brucellosis in tropical Africa. 3. Serological survey in Cameroon. Rev. Méd. Vét. 138: 55–58.Google Scholar
  3. Domenech J., Lucet P., Vallat B., Stewart C., Bonnet J.B., Bertaudiere L., 1980. Bovine brucellosis in central Africa. II. Clinical and epidemiological survey: regional characteristics and problems of semi-intensive husbandry. Rev. Elev. Méd. Vét. Pays Trop. 33: 277–284, Medline.PubMedGoogle Scholar
  4. Domenech J., Lucet P., Coudert M., 1982a. Bovine brucellosis in central Africa. V. Description of a simplified survey technique. Rev. Elev. Méd. Vét. Pays Trop. 35: 125–129, Medline.PubMedGoogle Scholar
  5. Domenech J., Lucet P., Vallat B., Stewart C., Bonnet J.B., Hentic A., 1982b. Brucellosis in cattle in central Africa. III. Statistical results of surveys in Chad and Cameroon. Rev. Elev. Méd. Vét. Pays Trop. 35: 15–22, Medline.PubMedGoogle Scholar
  6. Domenech J., Trap D., Gaumont R., 1985. Study of the reproductive disorders of cattle in central Africa: survey for chlamydiosis and Q fever. Rev. Elev. Méd. Vét. Pays Trop. 38: 138–143, Medline.PubMedGoogle Scholar
  7. Lefèvre P.C., 1991. Atlas des maladies infectieuses des ruminants. IEMVT-CIRAD, Maisons-Alfort. 95p.Google Scholar
  8. Ocholi R.A., Ezeokoli C.D., Akerejola O.O., Saror D.I., 1996. Use of the enzyme-linked immunosorbent assay for screening cattle for Brucella antibodies in Nigeria. Vet Q. 181:22–4 Medline, MarPubMedGoogle Scholar
  9. Omer M. K., Skjerve E., Holstad G., Woldehiwet Z., and Macmillan A. P., 2000. Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems. Epidemiology and Infection 125: 447–453. Cambridge University Press Medline DOI  10.1017/S0950268899004501 PubMedCrossRefGoogle Scholar
  10. Saz J.V., Beltran M., Diaz A., Agulla A., Merino F.J., Villasante P.A., Velasco A.C., 1987. Enzyme-linked immunosorbent assay for diagnosis of brucellosis. Eur J Clin Microbiol. 61:71–4, 1987 Feb.PubMedCrossRefGoogle Scholar
  11. Schelling E., Diguimbaye C., Daoud S., Nicolet J., Boerlin P., Tanner M., Zinsstag J., 2003. Brucellosis and Q-fever seroprevalences of nomadic pastoralists and their livestock in Chad. Prev Vet Med. 614:279–93, 2003 Dec 12 Medline. DOI  10.1016/j.prevetmed.2003.08.004 PubMedCrossRefGoogle Scholar
  12. Shey Njila O., 2004. A sero-epidemiological study of bovine brucellosis in the region of Dschang (West, Cameroon). MSc. Thesis ITM Antwerp, Belgium.Google Scholar
  13. Shey-Njila O., J. Awah-Ndukum, Bayemi P. H., Nyah E., Zoli P. A., Geerts S., 2005. Brucellosis in cameroon: current status and challenges for the future. The role of biotechnology in animal agriculture to address poverty in Africa: Opportunities and challenges. Arusha, Tanzania, 23–26 Septembre.Google Scholar
  14. SVANOVA, 2005. Brucella-Ab, C-ELISA SVANOVIRä. ELISA test for the detection of Brucella antibodies in serum samples discriminating between infected and vaccinated cattle. Manual.Google Scholar
  15. Tounkara K., Maiga S., Traore A., Seck B.M., Akakpo A.J., 1994. Epidemiology of bovine brucellosis in Mali: serologic investigation and initial isolation of strains of Brucella abortus. Rev Sci Tech. 133:777–86 Medline, 1994 SepPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • P. H. Bayemi
    • 1
  • E. C. Webb
    • 2
  • M. V. Nsongka
    • 1
  • H. Unger
    • 3
  • H. Njakoi
    • 4
  1. 1.Institute of Agricultural Research for Development Bambui (IRAD)BamendaCameroon
  2. 2.Department of Animal and Wildlife ScienceUniversity of PretoriaPretoriaSouth Africa
  3. 3.Animal Production and Health SectionInternational Atomic Energy Agency (IAEA)ViennaAustria
  4. 4.Heifer International CameroonLittle RockUSA

Personalised recommendations