Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon
Holstein cattle of a small scale dairy production systems were screened for Brucella abortus antibodies in 21 villages in Cameroon by ELISA. Results show a general seroprevalence of 8.4% in Holstein cattle. Of the 192 cows tested, 14 were infected giving a within-sex seroprevalence of 7.3% while 6/74 bulls were infected with a seroprevalence of 8%. There was no evidence (P=0.11) of differences in seroprevalence between age groups although animals above one year and below three years accounted for nearly half of the infected animals. 64% of infected animals were found in three locations (P=0.015): Kutaba (32%), Bamendankwe (16%) and Finge (16%). A specific control programme should be organized at these locations. Measures should be taken to ensure the eradication of the disease within the population and sound control measures adopted to avoid a further spread of the disease to larger cattle populations. Infected animals should be slaughtered systematically. All farmers should be advised to boil milk before consumption. Vaccination against Brucella abortus should be instituted and use of artificial insemination propagated. In order to ensure a productive and healthy population of Holstein cows within the dairy production scheme, regular Brucella testing should be instituted.
KeywordsBrucella Cameroon ELISA Holstein cattle Small scale dairy
World organization for animal health
enzyme-linked immunosorbent assay
This work was sponsored by the International Atomic Energy Agency (IAEA) and the Heifer International Cameroon. The authors are grateful to Mr Fonguh Emannuel, Mr Njong Vincent, Mr Apiembo for samples collection and laboratory work.
- Akakpo A.J., Bornarel P., 1987. Epidémiologie des brucelloses animales en Afrique tropicale:Enquête clinique, sérologique et bactériologique. Rev. Sci. Techn. Off. Int. Epiz. 6: 981–1027.Google Scholar
- Bornarel P., Akakpo A.J., Tuekam, 1987. Epidemiology of bovine brucellosis in tropical Africa. 3. Serological survey in Cameroon. Rev. Méd. Vét. 138: 55–58.Google Scholar
- Domenech J., Lucet P., Vallat B., Stewart C., Bonnet J.B., Bertaudiere L., 1980. Bovine brucellosis in central Africa. II. Clinical and epidemiological survey: regional characteristics and problems of semi-intensive husbandry. Rev. Elev. Méd. Vét. Pays Trop. 33: 277–284, Medline.PubMedGoogle Scholar
- Lefèvre P.C., 1991. Atlas des maladies infectieuses des ruminants. IEMVT-CIRAD, Maisons-Alfort. 95p.Google Scholar
- Omer M. K., Skjerve E., Holstad G., Woldehiwet Z., and Macmillan A. P., 2000. Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems. Epidemiology and Infection 125: 447–453. Cambridge University Press Medline DOI 10.1017/S0950268899004501 PubMedCrossRefGoogle Scholar
- Shey Njila O., 2004. A sero-epidemiological study of bovine brucellosis in the region of Dschang (West, Cameroon). MSc. Thesis ITM Antwerp, Belgium.Google Scholar
- Shey-Njila O., J. Awah-Ndukum, Bayemi P. H., Nyah E., Zoli P. A., Geerts S., 2005. Brucellosis in cameroon: current status and challenges for the future. The role of biotechnology in animal agriculture to address poverty in Africa: Opportunities and challenges. Arusha, Tanzania, 23–26 Septembre.Google Scholar
- SVANOVA, 2005. Brucella-Ab, C-ELISA SVANOVIRä. ELISA test for the detection of Brucella antibodies in serum samples discriminating between infected and vaccinated cattle. Manual.Google Scholar