Tropical Animal Health and Production

, Volume 40, Issue 7, pp 521–527 | Cite as

The detection of antibody against peste des petits ruminants virus in Sheep, Goats, Cattle and Buffaloes

  • Haider Ali Khan
  • Muhammad Siddique
  • Sajjad-ur-Rahman
  • Muhammad Abubakar
  • Muhammad Ashraf
Original Paper

Abstract

Monoclonal antibody-based competitive ELISA (C-ELISA) has been used for the specific measurement of antibodies to peste des petits ruminants (PPR) viruses in sheep, goats, cattle and Buffalo. Serum samples from sheep (n = 232), goats (n = 428), cattle (n = 43), buffalo (n = 89) were tested. The animals had not been vaccinated against rinderpest or PPR. Findings suggested that the sero-positive cases were significantly higher in sheep (51.29%) than in goats (39.02%) (P = 0.002). The overall sero-prevalence of PPRV in small ruminants was 43.33%. The PPR antibodies seroprevalence was 67.42% in buffalo and 41.86% in cattle which was significantly higher in buffalo (P = 0.005). The overall sero-prevalence of PPRV in large ruminants was 59.09%. Cattle and buffalo sera showed a high prevalence of antibody against PPR virus which may explain the difficulty experienced in achieving high post-vaccination immunity levels against rinderpest. Because antibodies against PPR virus are both cross-neutralizing and cross-protective against rinderpest virus, further vaccination in the presence of antibodies against PPR virus may be a waste of national resources. It was also suggested that antibodies to PPR virus could prevent an immune response to the rinderpest vaccine. This paper presents serological evidence for the transmission of PPR virus from sheep and goats to cattle and buffalo and highlights the need to include PPR serology in the sero-monitoring programme to give a better indication of national herd immunity of sheep and goats against PPR.

Keywords

Peste des petits ruminants cELISA Prevalence Sheep and goats Cattle and buffaloe 

References

  1. Abraham, G., Sintayehu, A., Libeau, G., Albina, E., Roger, F., Laekemariam, Y., Abayneh, D & Awoke, K.M. (2005). Antibody seroprevalences against peste des petits ruminants (PPR) virus in camels, cattle, goats and sheep in Ethiopia. Preventive Veterinary Medicine. 70: 51–57.PubMedCrossRefGoogle Scholar
  2. Anderson, E.C., Hassan, A., Barrett, T., & Anderson, J. (1990). Observations on the pathogenicity for sheep and goats and the transmissibility of the strain of virus isolated during the rinderpest outbreak in Sri Lanka in 1987. Veterinary Microbiology 21: 309–318.PubMedCrossRefGoogle Scholar
  3. Anderson, J. and McKay (1994). The detection of antibodies against PPR virus in cattle, sheep and goats and the possible implications to rinderpest control programme. Epidemiology and Infection. 112: 225–231.PubMedGoogle Scholar
  4. Athar, H., Islam, Q. U., Azim, F., Shakoor, A., Maqbool, A. & Chaudhary, N. I. (1995). An outbreak of peste des petits ruminants- like disease among goats in Punjab (Pakistan). Pakistan Veterinary Journal. 15: 140–143Google Scholar
  5. Barrett, T., Visser, I. K. G., Mamaev, L., Goatley, L., Bressem, M. F., & Van Osterhaus, A. D. M. (1993). Dolphine and porpoise morbilliviruses are genetically distinct from phocine distemper virus. Virology 193: 1010–1012.PubMedCrossRefGoogle Scholar
  6. Diallo, A., Barrett, T., Barbron, M., Subbarao, S. M. & Taylor, W. P. (1989). Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones. Journal of Virological Methods 23:127–136.PubMedCrossRefGoogle Scholar
  7. Dost, M. (1998). Grassland and Pasture Crops: Country Pasture/Forage Resourse Profile. Available from: www.fao.org/ag/AGP/AGPC/doc/Counprof/ Pakistan.htm. Accessed 2006 June12.Google Scholar
  8. Gibbs, P. J. E., Taylor, W. P., Lawman, M. P. & Bryant, J. (1979). Classification of the peste des petits ruminants virus as the fourth member of the genus Morbillivirus. Intervirology 11: 268–274.PubMedCrossRefGoogle Scholar
  9. Govindarajan, R., Koteeswaran, A., Venugopalan, A. T., Shyam, G. Shaouna, S., Shaila, M. S. & Ramachandran, S. (1997). Isolation of peste des petits ruminants virus from an outbreak in Indian buffalo (Buballus bubalus). Veterinary Record 141: 573–574.PubMedCrossRefGoogle Scholar
  10. Hussain, M., Afzal, M., Muneer, R., Ashfaq, M. and Haq, E. U. (1998). An outbreak of peste des petits ruminants in goats in Rawalpindi. Pakistan Veterinary Journal 18: 224–226.Google Scholar
  11. Lefevre, P. C. and Diallo, A. (1990). Peste des petites ruminants. Revue Scientifique Office International des Epizooties 9 (4) : 951–965.Google Scholar
  12. Ngangnou, A., Zoyem, N., Hamet, M. and Abdoulkadiri, S. (1996). Evaluation de la protection vaccinale contre la peste bovine au Cameroun III. Evaluation globale. Revue d’élevage et de médecine vétérinaire des pays tropicaux 49 (1) : 18–22.Google Scholar
  13. Ozkul, A., Akca,Y., Alkan, F., Barrett, T.,Karaoglu, T.,Daglap, S. B., Anderson, J., Yesilbag, K., Cokcaliskan, C., Genacy, A. and Burgu, I., (2002). Prevalence, distribution and host range of peste des petits ruminants virus, Turkey. Emerging Infectious Diseases 8 (7): 708–712.PubMedGoogle Scholar
  14. Roeder, P. L., Abraham, G., Kenfe and Barrett, T.(1994). PPR in Ethiopian goats. Tropical Animal Health and Production 26 (2): 69–70.PubMedCrossRefGoogle Scholar
  15. Roeder, P. L. and Obi, T. U. (1999). Recognizing Peste des Petites Ruminants: A Field Manual, Food and Agriculture Organization, Rome. Animal Health Manual pp. 5–28.Google Scholar
  16. Roger, F., Diallo, A., Yigezu, L. M., Hurard, C., Libeau, G.,Mebratu G. Y. and Faye, B. (2000). Investigation of a new pathological condition of camels in Ethiopia. Journal of Camel Practice & Research 2(7): 163–166.Google Scholar
  17. Saliki, J., Libeau, G., House, J. A., Mebus, A. and Dubov, E. J. (1993). Monoclonal antibody based blocking ELISA for specific detection and titration of PPR virus antibody in caprine and ovine sera. Journal Clinical Microbiology. 31 (5): 1075–1082.Google Scholar
  18. Shaila, M. S, Purushothaman, V., Bhavasar, D., Venugopal, K., Venkatesan, R. A. (1989). Peste des petits ruminants in India. Veterinary Record 125: 602.PubMedGoogle Scholar
  19. Shaila, M. S., David, S., Foryth, M. A., Diallo, A., Goatley, L., Kitching, R. P. & Barret, T. (1996). Geographical distribution and epidemiology of PPR viruses. Virus Research 43: 149–153.PubMedCrossRefGoogle Scholar
  20. Singh, R. P., Sreenivasa, B. P., Dhar, P., Shah, L. C. & Bandyopadhyay, S. K. (2004). Development of a monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petits ruminants (PPR) virus. Veterinary Microbiology 98: 3–15.PubMedCrossRefGoogle Scholar
  21. Taylor, W. P., (1984). The distribution and epidemiology of PPR. Preventive Veterinary Medicine. 2: 157–166.CrossRefGoogle Scholar
  22. Taylor W.P., Al Busaidy, S. & Barrett, T. (1990). The epidemiology of peste des petits ruminants in the Sultanate of Oman. Veterinary Microbiology 22(4): 341–352.PubMedCrossRefGoogle Scholar
  23. Taylor W.P., Diallo, A., Gopalkrishna, S., Sreeramalu, P., Wilsore, A. J., Nanda, Y. P., Libeau, G., Rajasekhar, M., and Mukhopadhyay, A. K. (2002). Peste des petits ruminants has been widely present in southern India since, if not before, the late 1980s. Prev Vet Med 52: 305–12PubMedCrossRefGoogle Scholar
  24. Tounkara, K., Traore, A., Traore, A. P., Sidibe, S., Samake, Diallo, B.O. & Diallo, A. (1996). Epidémiologie de la peste des petits ruminants (PPR) et de la peste bovine au Mali: enquètes sérologiques. Revue d’élevage et de médecine vétérinaire des pays tropicaux 49 (4): 273–277.PubMedGoogle Scholar
  25. Wamwayi, H. M., Rossiter, P. B., Kariuki, D. P., Wafula, J. S., Barrett, T. and Anderson, J. (1995). Peste des petits ruminants antibodies in East Africa. Veterinary Record 136: 199–200.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Haider Ali Khan
    • 1
  • Muhammad Siddique
    • 1
  • Sajjad-ur-Rahman
    • 1
  • Muhammad Abubakar
    • 2
  • Muhammad Ashraf
    • 1
  1. 1.Department of Veterinary Microbiology, Faculty of Veterinary ScienceUniversity of AgricultureFaisalabadPakistan
  2. 2.ELISA GroupNational Veterinary Laboratory, NARCIslamabadPakistan

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