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Transgenic pigs expressing β-xylanase in the parotid gland improve nutrient utilization

  • Mao Zhang
  • Gengyuan Cai
  • Enqing Zheng
  • Guangguang Zhang
  • Yang Li
  • Zicong Li
  • Huaqiang YangEmail author
  • Zhenfang WuEmail author
Original Paper
  • 15 Downloads

Abstract

Xylan is one of the main anti-nutritional factors in pig’s feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo can improve digestibility of dietary xylan and absorption of nutrients, we produced transgenic pigs which express the xynB from Aspergillus Niger CGMCC1067 in the parotid gland via nuclear transfer. In four live transgenic founders, β-xylanase activities in the saliva were 0.74, 0.59, 0.37 and 0.24 U/mL, respectively. Compared with non-transgenic pigs, the content of crude protein (CP) in feces reduced by 15.5% (P < 0.05). Furthermore, in 100 of the 271 F1 pigs the xynB gene was detectable. The digestibility of gross energy and CP in F1 transgenic pigs were increased by 5% and 22%, respectively, with the CP content in feces decreased by 6.4%. Taken together, our study showed that the transgenic pigs producing β-xylanase from parotid gland can reduce the anti-nutritional effect in animal diet and improve the utilization of nutrients.

Keywords

β-xylanase Parotid gland Transgenic pigs 

Notes

Acknowledgements

This work was supported by grants from the National Science and Technology Major Project for Breeding of New Transgenic Organisms (2016ZX08006002), the National Natural Science Foundation of China (31772555), and the Education and research projects for junior faculty in Fujian Provincial Department of Education (JA1550).

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.National Engineering Research Center for Breeding Swine Industry, College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina
  2. 2.College of Life ScienceLongyan UniversityLongyanChina

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