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Increased multiple virus resistance in transgenic soybean overexpressing the double-strand RNA-specific ribonuclease gene PAC1

  • Xiangdong Yang
  • Lu Niu
  • Wei Zhang
  • Hongli He
  • Jing Yang
  • Guojie Xing
  • Dongquan Guo
  • Qianqian Zhao
  • Xiaofang Zhong
  • Haiyun Li
  • Qiyun Li
  • Yingshan Dong
Original Paper
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Abstract

Viruses constitute a major constraint to soybean production worldwide and are responsible for significant yield losses every year. Although varying degrees of resistance to specific viral strains has been identified in some soybean genetic sources, the high rate of mutation in viral genomes and mixed infections of different viruses or strains under field conditions usually hinder the effective control of viral diseases. In the present study, we generated transgenic soybean lines constitutively expressing the double-strand RNA specific ribonuclease gene PAC1 from Schizosaccharomyces pombe to evaluate their resistance responses to multiple soybean-infecting virus strains and isolates. Resistance evaluation over three consecutive years showed that the transgenic lines displayed significantly lower levels of disease severity in field conditions when challenged with soybean mosaic virus (SMV) SC3, a prevalent SMV strain in soybean-growing regions of China, compared to the non-transformed (NT) plants. After inoculation with four additional SMV strains (SC7, SC15, SC18, and SMV-R), and three isolates of bean common mosaic virus (BCMV), watermelon mosaic virus (WMV), and bean pod mottle virus (BPMV), the transgenic plants exhibited less severe symptoms and enhanced resistance to virus infections relative to NT plants. Consistent with these results, the accumulation of each virus isolate was significantly inhibited in transgenic plants as confirmed by quantitative real-time PCR and double antibody sandwich enzyme-linked immunosorbent assays. Collectively, our results showed that overexpression of PAC1 can increase multiple virus resistance in transgenic soybean, and thus provide an efficient control strategy against RNA viruses such as SMV, BCMV, WMV, and BPMV.

Keywords

Soybean Multiple virus resistance Soybean mosaic virus Bean common mosaic virus Watermelon mosaic virus Bean pod mottle virus 

Notes

Acknowledgements

This work was supported by Grants from China National Novel Transgenic Organisms Breeding Project (2016ZX08004-004), Jilin Provincial Agricultural Science & Technology Innovation Project (CXGC2017JQ013) and National Natural Science foundation of China (31671764). We thank Prof. Haijian Zhi (Nanjing Agricultural University in China) for providing virus strains and isolates and Prof. Shifang Li (Chinese Academy of Agricultural Sciences, China) for providing the plasmid pBI121-PAC1. We would also like to thank Editage (www.editage.cn) for English language editing.

Author Contributions

Yingshan Dong and Qiyun Li designed the experiments. Xiangdong Yang and Lu Niu conducted the experiments and drafted the manuscript. Jing Yang, Guojie Xing, Dongquan Guo, Qianqian Zhao, and Haiyun Li performed A. tumefaciens-mediated transformation experiments. Wei Zhang conducted the virus inoculation assays. Hongli He and Xiaofang Zhong participated in the qRT-PCR analyses. All authors participated in the manuscript revision.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest in the publication of this paper.

Supplementary material

11248_2018_108_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 66 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Xiangdong Yang
    • 1
  • Lu Niu
    • 1
  • Wei Zhang
    • 1
  • Hongli He
    • 1
  • Jing Yang
    • 1
  • Guojie Xing
    • 1
  • Dongquan Guo
    • 1
  • Qianqian Zhao
    • 1
  • Xiaofang Zhong
    • 1
  • Haiyun Li
    • 1
  • Qiyun Li
    • 1
  • Yingshan Dong
    • 1
  1. 1.Jilin Provincial Key Laboratory of Agricultural BiotechnologyJilin Academy of Agricultural SciencesChangchunChina

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