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Transgenic Research

, Volume 26, Issue 6, pp 775–789 | Cite as

High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza

  • Yanaysi Ceballo
  • Kenia Tiel
  • Alina López
  • Gleysin Cabrera
  • Marlene Pérez
  • Osmany Ramos
  • Yamilka Rosabal
  • Carlos Montero
  • Rima Menassa
  • Ann Depicker
  • Abel Hernández
Original Paper

Abstract

Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes. Two regulatory sequences of seed storage protein genes from Phaseolus vulgaris L. were used to direct the expression, yielding 3.0 mg of the viral antigen per g of seeds. The production and stability of seed-produced recombinant HA protein was characterized by different molecular techniques. The aqueous extract of tobacco seed proteins was used for subcutaneous immunization of chickens, which developed antibodies that inhibited the agglutination of erythrocytes after the second application of the antigen. The feasibility of using tobacco seeds as a vaccine carrier is discussed.

Keywords

Avian influenza Tobacco seed Hemagglutinin Phaseolin promoter 

Notes

Acknowledgements

We thank the Department of Plant Systems Biology of Ghent University Belgium for supplying the signals for expression in seeds. In addition, we would like to extend our appreciation to the staff of the National Centre for Animal and Plant Health (CENSA), Cuba for their help with immunization of chickens.

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Yanaysi Ceballo
    • 1
  • Kenia Tiel
    • 1
  • Alina López
    • 1
  • Gleysin Cabrera
    • 2
  • Marlene Pérez
    • 1
  • Osmany Ramos
    • 1
  • Yamilka Rosabal
    • 1
  • Carlos Montero
    • 3
  • Rima Menassa
    • 4
  • Ann Depicker
    • 5
    • 6
  • Abel Hernández
    • 1
  1. 1.Plant Biotechnology DepartmentCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba
  2. 2.Department of Carbohydrate ChemistryCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba
  3. 3.Animal Biotechnology DepartmentCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba
  4. 4.London Research and Development CentreAgriculture and Agri-Food CanadaLondonCanada
  5. 5.Department Plant Biotechnology and BioinformaticsGhent UniversityGhentBelgium
  6. 6.Department Plant Systems BiologieVIBGhentBelgium

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