CRISPR/Cas9: an advanced tool for editing plant genomes
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To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.
KeywordsSequence-specific nucleases Genome editing CRISPR/Cas9 TALENs ZFNs Plants
We thank Emily Maclary (Department of human genetics, University of Michigan, USA) for editing the language of the manuscript. We are grateful to Prof. Soumitra Kumar Sen for his guidance and inspiration. We thank the funding agencies Indian Council of Agricultural Research (ICAR) and Department of Science and Technology (DST) for financial support.
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Conflict of interest
The authors declare that they have no conflict of interest.
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