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CRISPR/Cas9: an advanced tool for editing plant genomes

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Abstract

To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.

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Acknowledgments

We thank Emily Maclary (Department of human genetics, University of Michigan, USA) for editing the language of the manuscript. We are grateful to Prof. Soumitra Kumar Sen for his guidance and inspiration. We thank the funding agencies Indian Council of Agricultural Research (ICAR) and Department of Science and Technology (DST) for financial support.

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  1. Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    Milan Kumar Samanta & Avishek Dey

  2. Department of Human Genetics, University of Michigan, Ann Arbor, MI, 48109, USA

    Srimonta Gayen

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  1. Milan Kumar Samanta
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  2. Avishek Dey
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Correspondence to Srimonta Gayen.

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Samanta, M.K., Dey, A. & Gayen, S. CRISPR/Cas9: an advanced tool for editing plant genomes. Transgenic Res 25, 561–573 (2016). https://doi.org/10.1007/s11248-016-9953-5

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