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Low frequency paternal transmission of plastid genes in Brassicaceae

Abstract

Plastid-encoded genes are maternally inherited in most plant species. Transgenes located on the plastid genome are thus within a natural confinement system, preventing their distribution via pollen. However, a low-frequency leakage of plastids via pollen seems to be universal in plants. Here we report that a very low-level paternal inheritance in Arabidopsis thaliana occurs under field conditions. As pollen donor an Arabidopsis accession (Ler-Ely) was used, which carried a plastid-localized atrazine resistance due to a point mutation in the psbA gene. The frequency of pollen transmission into F1 plants, based on their ability to express the atrazine resistance was 1.9 × 10−5. We extended our analysis to another cruciferous species, the world-wide cultivated crop Brassica napus. First, we isolated a fertile and stable plastid transformant (T36) in a commercial cultivar of B. napus (cv Drakkar). In T36 the aadA and the bar genes were integrated in the inverted repeat region of the B. napus plastid DNA following particle bombardment of hypocotyl segments. Southern blot analysis confirmed transgene integration and homoplasmy of plastid DNA. Line T36 expressed Basta resistance from the inserted bar gene and this trait was used to estimate the frequency of pollen transmission into F1 plants. A frequency of <2.6 × 10−5 was determined in the greenhouse. Taken together, our data show a very low rate of paternal plastid transmission in Brassicacea. Moreover, the establishment of plastid transformation in B. napus facilitates a safe use of this important crop plant for plant biotechnology.

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Acknowledgments

This research was supported by the Bundesministerium für Bildung und Forschung (Germany) (Förderkennzeichen 0315212C). We gratefully acknowledge that Icon Genetics AG (Freising-Weihenstephan, Germany) made their protocol for plastid transformation available for this study. We thank Prof. Koornneef for Arabidopsis seeds and Dr. Liudmyla Sakhno for B. napus seeds, and Amira Zribi and the greenhouse team for taking care of the rapeseed plants.

Conflict of interest

All authors state that they have no conflicts of interest.

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Correspondence to Dario Leister.

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Schneider, A., Stelljes, C., Adams, C. et al. Low frequency paternal transmission of plastid genes in Brassicaceae . Transgenic Res 24, 267–277 (2015). https://doi.org/10.1007/s11248-014-9842-8

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Keywords

  • Arabidopsis thaliana
  • Brassica napus
  • Glufosinate resistance
  • Paternal inheritance
  • Plastid transformation
  • Plastid inheritance
  • Transgene containment