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Transgenic Research

, Volume 23, Issue 1, pp 89–97 | Cite as

The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae

  • Yanrong Zhou
  • Yanli Lin
  • Xiaojie Wu
  • Chong Feng
  • Chuan Long
  • Fuyin Xiong
  • Ning Wang
  • Dengke PanEmail author
  • Hongxing ChenEmail author
Original Paper

Abstract

Livestock meat is generally low in n-3 polyunsaturated fatty acids (PUFAs), which are beneficial to human health. An alternative approach to increasing the levels of n-3 PUFAs in meat is to generate transgenic livestock animals. In this study, we describe the generation of cloned pigs that express the cbr-fat-1 gene from Caenorhabditis briggsae, encoding an n-3 fatty acid desaturase. Analysis of fatty acids demonstrated that the cbr-fat-1 transgenic pigs produced high levels of n-3 fatty acids from n-6 analogs; consequently, a significantly reduced ratio of n-6/n-3 fatty acids was observed. We demonstrated that the n-3 desaturase gene from C. briggsae was functionally expressed, and had a significant effect on the fatty acid composition of the transgenic pigs, which may allow the production of pork enriched in n-3 PUFAs.

Keywords

n-3 fatty acid desaturase cbr-fat-1 Transgenic pig Somatic cell nuclear transfer 

Notes

Acknowledgments

This research was supported by the China Major National Science and Technology Project Grant 2013zx08006-002.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yanrong Zhou
    • 1
  • Yanli Lin
    • 1
  • Xiaojie Wu
    • 1
  • Chong Feng
    • 2
  • Chuan Long
    • 2
  • Fuyin Xiong
    • 1
  • Ning Wang
    • 2
  • Dengke Pan
    • 2
    Email author
  • Hongxing Chen
    • 1
    Email author
  1. 1.Cell Engineering DepartmentBeijing Institute of BiotechnologyBeijingPeople’s Republic of China
  2. 2.Gene and Cell Engineering Department, Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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