We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked chloramphenicol acetyltransferase gene, and examined the Cre activity in the Tg mouse brains by assessing β-galactosidase activity. Cre activity was specifically detected in the caudate-putamen, nucleus accumbens, and olfactory tubercle of the Gpr88-Cre Tg mouse brain. Medium spiny neurons within the caudate-putamen exhibited Cre activity. Thus, Gpr88-Cre Tg mice could be a useful tool for analyzing the function of the basal ganglia by using Cre/loxP systems.
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We thank CHRCO for a BAC clone (RP23-99k15), and Dr. Jun-ichi Miyazaki for providing the CAG-CAT-Z Tg mice. We also thank Dr. Shigeyoshi Itohara and Dr. Yoshikazu Saito (Lab. for Behavioral Genetics, RIKEN) for the NLS-Cre-poly(A) cassette and technical advice and help, and Dr. Takashi Arai (Support unit for Animal Resources Development, RIKEN) for generation of Tg mice. We also thank all member of our laboratory, especially Dr. Katsuhiro Kawaai, Dr. Takeyuki Sugawara, and Ms. Etsuko Ebisui for their kind help. Supported by The Moritani Scholarship Foundation (C. H), JSPS KAKENHI Grant Numbers, 20500301 (C. H), and 20220007 (K. M) and The Japan Science and Technology Agency (K. M).
Conflict of interest
The authors have no conflicts of interest to declare.
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Hisatsune, C., Ogawa, N. & Mikoshiba, K. Striatum-specific expression of Cre recombinase using the Gpr88 promoter in mice. Transgenic Res 22, 1241–1247 (2013). https://doi.org/10.1007/s11248-013-9711-x
- Transgenic mice
- Bacterial artificial clone
- Basal ganglia