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Transgenic Research

, Volume 22, Issue 6, pp 1231–1239 | Cite as

Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease

  • Jianhua Ye
  • Jin He
  • Qiuyan Li
  • Yuanyuan Feng
  • Xueyuan Bai
  • Xiangmei Chen
  • Yaofeng Zhao
  • Xiaoxiang Hu
  • Zhengquan Yu
  • Ning LiEmail author
Brief Communication

Abstract

After several decades of research, autosomal dominant polycystic kidney disease (ADPKD) is still incurable and imposes enormous physical, psychological, and economic burdens on patients and their families. Murine models of ADPKD represent invaluable tools for studying this disease. These murine forms of ADPKD can arise spontaneously, or they can be induced via chemical or genetic manipulations. Although these models have improved our understanding of the etiology and pathogenesis of ADPKD, they have not led to effective treatment strategies. The mini-pig represents an effective biomedical model for studying human diseases, as the pig’s human-like physiological processes help to understand disease mechanisms and to develop novel therapies. Here, we tried to generate a transgenic model of ADPKD in pigs by overexpressing c-Myc in kidney tissue. Western-blot analysis showed that c-Myc was overexpressed in the kidney, brain, heart, and liver of transgenic pigs. Immunohistochemical staining of kidney tissue showed that exogenous c-Myc predominantly localized to renal tubules. Slightly elevated blood urea nitrogen levels were observed in transgenic pigs 1 month after birth, but no obvious abnormalities were detected after that time. In the future, we plan to subject this model to renal injury in an effort to promote ADPKD progression.

Keywords

ADPKD c-Myc Pig Disease model 

Notes

Acknowledgments

This study was funded by the Chinese National Basic Research Program (“973” Program) (Project No. 2011CB944100).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jianhua Ye
    • 1
  • Jin He
    • 1
  • Qiuyan Li
    • 1
  • Yuanyuan Feng
    • 2
  • Xueyuan Bai
    • 2
  • Xiangmei Chen
    • 2
  • Yaofeng Zhao
    • 1
  • Xiaoxiang Hu
    • 1
  • Zhengquan Yu
    • 1
  • Ning Li
    • 1
    Email author
  1. 1.State Key Laboratory for AgrobiotechnologyChina Agricultural UniversityBeijingChina
  2. 2.Department of Nephrology, State Key Laboratory of Kidney DiseasesChinese P.L.A. General HospitalBeijingChina

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