Transgenic Research

, Volume 22, Issue 3, pp 607–619 | Cite as

Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori)

  • Jianping Duan
  • Hanfu Xu
  • Sanyuan Ma
  • Huizhen Guo
  • Feng Wang
  • Ping Zhao
  • Qingyou Xia
Original Paper

Abstract

Cre-mediated recombination is widely used to manipulate defined genes spatiotemporally in vivo. The present study evaluated the Cre/loxP system in Bombyx mori by establishing two transgenic lines. One line contained a Cre recombinase gene controlled by a sericin-1 gene (Ser1) promoter. The other line contained a loxP-Stop-loxP-DsRed cassette driven by the same Ser1 promoter. The precise deletion of the Stop fragment was found to be triggered by Cre-mediated site-specific excision, and led to the expression of DsRed fluorescence protein in the middle silk glands of all double-transgenic hybrids. This result was also confirmed by phenotypical analysis. Hence, the current study demonstrated the feasibility of Cre-mediated site-specific recombination in B. mori, and opened a new window for further refining genetic tools in silkworms.

Keywords

Cre/loxP Site-specific excision Stop fragment Targeted gene activation B. mori 

Abbreviations

Ser1

Sericin-1 gene

floxed

Flanked by two loxP sites

RFP

DsRed fluorescence protein

pA

SV40 polyadenylation signal

AMSG

Anterior subpart of middle silk gland

MMSG

Middle subpart of middle silk gland

PMSG

Posterior subpart of middle silk gland

MSG

Middle silk gland

PSG

Posterior silk gland

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Jianping Duan
    • 1
  • Hanfu Xu
    • 1
  • Sanyuan Ma
    • 1
  • Huizhen Guo
    • 1
  • Feng Wang
    • 1
  • Ping Zhao
    • 1
  • Qingyou Xia
    • 1
  1. 1.State Key Laboratory of Silkworm Genome BiologySouthwest UniversityChongqingChina

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