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Transgenic Research

, Volume 22, Issue 4, pp 849–859 | Cite as

Characterization of stable fluorescent transgenic marine medaka (Oryzias dancena) lines carrying red fluorescent protein gene driven by myosin light chain 2 promoter

  • Young Sun Cho
  • Sang Yoon Lee
  • Dong Soo Kim
  • Yoon Kwon NamEmail author
Brief Communication

Abstract

Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a single copy to more than 230 copies per genome. Although the transgenic founders displayed mosaic and/or ectopic expression of the RFP signal, the resultant F1 transgenics and their progeny showed consistently stable transmission of the transgenic locus and uniform RFP signal through several subsequent generations. In adult transgenics, an authentic brilliant red fluorescence was achieved over the skeletal muscles of the transgenic individuals, which might be sufficient for ornamental display. Expression analysis of the transgenic mRNAs indicated that rfp transcripts were predominantly expressed in the skeletal muscles. Different transgenic lines displayed different levels of transgene expression at the mRNA, protein, and phenotypic levels. However, the efficiency of transgene expression was independent of the transgene copy number. The RFP protein levels were consistently stable in the transgenic fish muscles through several generations, up to F5. The results of this study suggest that transgenic marine medaka that acquire strong fluorescent signals in their skeletal muscles can be developed as a promising, novel ornamental fish for display in both freshwater and seawater aquaria.

Keywords

Myosin light chain 2 promoter rfp Marine medaka Oryzias dancena Fluorescent expression Transgenic germline 

Notes

Acknowledgments

This study was supported by a research fund from the Ministry of Land, Transport and Maritime Affairs, Korea (project #20088033-1).

Supplementary material

11248_2012_9675_MOESM1_ESM.docx (2.6 mb)
Supplementary material 1 (DOCX 2689 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Young Sun Cho
    • 1
  • Sang Yoon Lee
    • 2
  • Dong Soo Kim
    • 1
    • 2
  • Yoon Kwon Nam
    • 1
    • 2
    Email author
  1. 1.Institute of Marine Living Modified OrganismsPukyong National UniversityBusanKorea
  2. 2.Department of Marine Bio-Materials and AquaculturePukyong National UniversityBusanKorea

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