Transgenic Research

, Volume 22, Issue 3, pp 621–629 | Cite as

High-level production of lactostatin, a hypocholesterolemic peptide, in transgenic rice using soybean A1aB1b as carrier

  • Cerrone Cabanos
  • Atsushi Ekyo
  • Yoshiki Amari
  • Naoki Kato
  • Masaharu Kuroda
  • Satoshi Nagaoka
  • Fumio Takaiwa
  • Shigeru Utsumi
  • Nobuyuki Maruyama
Original Paper

Abstract

Hypercholesterolemia, a form of cardiovascular disease, is one of the leading causes of deaths worldwide. Lactostatin (Ile-Ile-Ala-Glu-Lys), derived from β-lactoglobulin in cow’s milk, is a bioactive peptide with hypocholesterolemic activity higher than sitosterol, a known anti-hypercholesterolemic drug. Here, we successfully developed a transgenic rice accumulating a much higher level of lactostatin by inserting 29 IIAEK sequences into the structurally flexible (nonconserved) regions of soybean seed storage protein, A1aB1b, and introducing it into LGC-1 (low glutelin content mutant 1) as host variety. A1aB1b containing 29 lactostatins was expressed in the endosperm of rice seed cells by using seed specific promoters and sorted into novel compartments distinct from normal PB-I (ER-derived protein body) and PB-II (protein storage vacuoles). Transgenic rice seeds accumulated approximately 2 mg of lactostatins/g of dry seeds, which is relatively high compared with previous reports. Our findings suggest that the introduction of a high copy number of bioactive peptide into seed storage proteins as carrier is one of the effective means in producing higher amounts of bioactive peptides in rice.

Keywords

Bioactive peptide Rice Seed storage protein Lactostatin 

Notes

Acknowledgments

We thank DASH (Development and Assessment of Sustainable Humanosphere) system and Prof. Kazufumi Yazaki of Kyoto University for supporting the development of the transgenic crop. We also thank Prof. Reiko Urade (Kyoto University) for the warm encouragement and Ms. M. Sawada and Ms E. Okuda for technical assistance. This research was supported by the research grant, “Genomics and Agricultural Innovation, GMC0008” from the Ministry of Agriculture, Forestry and Fisheries of Japan to M. Kuroda, S. Nagaoka, F. Takaiwa, S. Utsumi and N. Maruyama, and the Asahi Glass Foundation to N Maruyama and S Utsumi.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Cerrone Cabanos
    • 1
  • Atsushi Ekyo
    • 1
  • Yoshiki Amari
    • 1
  • Naoki Kato
    • 1
  • Masaharu Kuroda
    • 2
  • Satoshi Nagaoka
    • 3
  • Fumio Takaiwa
    • 4
  • Shigeru Utsumi
    • 1
  • Nobuyuki Maruyama
    • 1
  1. 1.Laboratory of Food Quality Design and Development, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Rice Physiology Research TeamNational Agricultural Research CenterJoetsuJapan
  3. 3.Department of Applied Life Science, Faculty of Applied Biological SciencesGifu UniversityGifuJapan
  4. 4.Functional Crop Research and Development UnitNational Institute of Agrobiological SciencesTsukubaJapan

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