Transgenic Research

, Volume 22, Issue 1, pp 195–200 | Cite as

Production of mouse pups from germline transmission-failed knockout chimeras

  • Yoshitaka Fujihara
  • Kazuhiro Kaseda
  • Naokazu Inoue
  • Masahito Ikawa
  • Masaru OkabeEmail author
Technical Report


Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.


ES cells C57BL/6N Transgenic mice EGFP ICSI 



We thank Y. Esaki, K. Kawata and Y. Koreeda for technical support. This work was partly supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Supplementary material

11248_2012_9635_MOESM1_ESM.pdf (189 kb)
Supplementary material 1 (PDF 188 kb)


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yoshitaka Fujihara
    • 1
  • Kazuhiro Kaseda
    • 1
  • Naokazu Inoue
    • 1
  • Masahito Ikawa
    • 1
  • Masaru Okabe
    • 1
    Email author
  1. 1.Research Institute for Microbial DiseasesOsaka UniversitySuitaJapan

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