Transgenic Research

, Volume 21, Issue 4, pp 785–795 | Cite as

Over-expression of OSRIP18 increases drought and salt tolerance in transgenic rice plants

  • Shu-Ye Jiang
  • Ritu Bhalla
  • Rengasamy Ramamoorthy
  • Hong-Fen Luan
  • Prasanna Nori Venkatesh
  • Minne Cai
  • Srinivasan RamachandranEmail author
Original Paper


Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for such improvement. Here, we have investigated the biological functions of an Oryza sativa Ribosome-inactivating protein gene 18 (OSRIP18) by ectopically expressing this gene under the control of CaMV 35S promoter in the rice genome. We have generated 11 independent transgenic rice plants and all of them showed significantly increased tolerance to drought and high salinity stresses. Global gene expression changes by Microarray analysis showed that more than 100 probe sets were detected with up-regulated expression abundance while signals from only three probe sets were down-regulated after over-expression of OSRIP18. Most of them were not regulated by drought or high salinity stresses. Our data suggested that the increased tolerance to these abiotic stresses in transgenic plants might be due to up-regulation of some stress-dependent/independent genes and OSRIP18 may be potentially useful in further improving plant tolerance to various abiotic stresses by over-expression.


Drought stress Ectopic over-expression High salinity stress Ribosome-inactivating protein Rice 



Megnaporthe grisea


Polyethylene glycol


Quantitative real-time RT-PCR


Rapid amplification of cDNA ends


Ribosome-inactivating proteins


Wild type


Xanthomonas oryzae pv oryzae

Supplementary material

11248_2011_9568_MOESM1_ESM.pdf (202 kb)
Supplementary material 1 (PDF 202 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Shu-Ye Jiang
    • 1
  • Ritu Bhalla
    • 1
    • 2
  • Rengasamy Ramamoorthy
    • 1
  • Hong-Fen Luan
    • 1
  • Prasanna Nori Venkatesh
    • 1
  • Minne Cai
    • 1
  • Srinivasan Ramachandran
    • 1
    Email author
  1. 1.Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research LinkThe National University of SingaporeSingaporeSingapore
  2. 2.Republic PolytechnicSingaporeSingapore

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