Production of cloned transgenic cow expressing omega-3 fatty acids
n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.
KeywordsN-3 fatty acid desaturase gene mfat-1 Transgenic cattle Nuclear transfer
- Indo Y, Tatemizo A, Abe Y, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K (2009) Functional expression of a humanized gene for an ω-3 fatty acid desaturase from scarlet flax in transfected bovine adipocytes and bovine embryos cloned from the cells. Biochim Biophys Acta 1791:183–190PubMedGoogle Scholar
- Marmer WN, Maxwell RJ, Williams JE (1984) Effects of dietary regimen and tissue site on bovine fatty acid profiles. J Anim Sci 59:109–121Google Scholar
- Saeki K, Matsumoto K, Kinoshita M, Suzuki I, Tasaka Y, Kano K, Taguchi Y, Mikami K, Hirabayashi M, Kashiwazaki N, Hosoi Y, Murata N, Iritani A (2004) Functional expression of a Delta12 fatty acid desaturase gene from spinach in transgenic pigs. Proc Natl Acad Sci USA 101:6361–6366PubMedCrossRefGoogle Scholar
- Tao F, Zhu SW, Fan J, Cheng BJ (2006) Cloning and sequence analysis of maize FAD2 gene. Chin J Plant Physiol Mol Biol 32:649–656Google Scholar