Production of cloned transgenic cow expressing omega-3 fatty acids
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n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.
KeywordsN-3 fatty acid desaturase gene mfat-1 Transgenic cattle Nuclear transfer
This work was supported by Hi-Tech Research and Development Program of China (863 Programs, Nos. 2007AA100505 and 2008AA10Z159) and grant from Inner Mongolia University for Chang Jiang Scholars Program to GPL.
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