Transgenic Research

, Volume 21, Issue 3, pp 567–578 | Cite as

Production of Phytophthorainfestans-resistant potato (Solanum tuberosum) utilising Ensifer adhaerens OV14

Original Paper

Abstract

Based on the use of Agrobacterium tumefaciens-mediated transformation commodity crop improvement through genetic engineering is the fastest adopted crop technology in the world (James 2010). However, the complexity of the Agrobacterium patent landscape remains a challenge for non-patent holders who wish to generate novel varieties for a commercial purpose. The potential of non-Agrobacterium strains (Transbacter) to modify a plant genome has previously been described. However, they are unlikely to be widely used without significant adjustments in transformation protocols in order to improve their gene transfer efficiencies. In this study we set out to identify alternative bacteria species that could (a) utilize vir genes for genetic transformation and (b) substitute for A. tumefaciens in existing transformation protocols, without a prerequisite for protocol modifications. To this end we isolated a collection (n = 751) of plant-associated bacteria from the rhizosphere of commercially grown crops. Based on various screens, including plant transformation with the open-source vector pCAMBIA5105, we identified a strain of the bacterium Ensifer adhaerens with the capacity to transform both Arabidopsis thaliana (0.12%) and potato (mean transformation frequency 35.1%). Thereafter, Ensifer adhaerens was used to generate blight- (causative organism Phytophthora infestans) resistant potato using the Solanum bulbocastanum ‘resistance to blight’ (RB) gene. Resistant genotypes were confirmed by associated molecular analysis and resistant phenotypes demonstrated by the development of hypersensitive lesions on inoculated leaf tissue post-pathogen inoculation. These data confirm the potential of Ensifer-mediated transformation (EMT) as a novel platform for the high frequency generation of transgenic potato.

Keywords

Ensifer adhaerens Transformation Non-Agrobacterium strains Solanum tuberosum Transgenic Phytophthora infestans 

Notes

Acknowledgments

The authors wish to acknowledge the Teagasc Core Research Program for funding this research and the Teagasc Walsh Fellowship Scheme for providing a stipend for T. W.

Supplementary material

11248_2011_9553_MOESM1_ESM.doc (748 kb)
Supplementary material 1 (DOC 749 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of Crop ScienceTeagasc Crops Research CentreOak ParkIreland
  2. 2.School of Biology and Environmental SciencesUniversity College DublinDublin 4Ireland
  3. 3.Faculty of Agricultural Sciences, Department of Genetics and BiotechnologyAarhus UniversitySlagelseDenmark

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