Transgenic Research

, Volume 21, Issue 1, pp 217–224 | Cite as

Generation of mice with a conditional Stat1 null allele

  • Barbara Wallner
  • Nicole R. Leitner
  • Raimund M. Vielnascher
  • Elisabeth Kernbauer
  • Thomas Kolbe
  • Marina Karaghiosoff
  • Thomas Rülicke
  • Thomas Decker
  • Mathias Müller
Brief Communication

Abstract

Interferons (IFNs) are key cytokines in the innate immune response that also bridge the gap to adaptive immunity. Signaling upon stimulation by IFN type I, II and III is mediated by the Jak-Stat pathway. STAT1 is activated by all three IFN receptor complexes and absence of STAT1 from mice increases their susceptibility to pathogens. In addition, depending on the setting, STAT1 can act as tumor suppressor or oncogene. Here we report the generation and detailed functional characterization of a conditional Stat1 knockout mouse. We show the integrity of the conditional Stat1 locus and report successful in vivo deletion by means of a ubiquitous and a tissue-specific Cre recombinase. The conditional Stat1 null allele represents an important tool for identifying novel and cell-autonomous STAT1 functions in infection and cancer.

Keywords

Stat1 Conditional knockout Cre recombinase Interferon 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Barbara Wallner
    • 1
  • Nicole R. Leitner
    • 1
  • Raimund M. Vielnascher
    • 1
  • Elisabeth Kernbauer
    • 2
  • Thomas Kolbe
    • 3
    • 4
  • Marina Karaghiosoff
    • 1
  • Thomas Rülicke
    • 3
    • 5
  • Thomas Decker
    • 2
  • Mathias Müller
    • 1
    • 3
  1. 1.Institute of Animal Breeding and GeneticsUniversity of Veterinary MedicineViennaAustria
  2. 2.Max F. Perutz Laboratories, Department of Genetics, Microbiology and ImmunobiologyUniversity of ViennaViennaAustria
  3. 3.University Center Biomodels Austria (BIAT)University of Veterinary MedicineViennaAustria
  4. 4.Department IFA-Tulln, Biotechnology in Animal ProductionUniversity of Natural Resources and Applied Life SciencesTullnAustria
  5. 5.Institute of Laboratory Animal SciencesUniversity of Veterinary MedicineViennaAustria

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