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Transgenic Research

, Volume 21, Issue 1, pp 225–226 | Cite as

Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs

  • Masato OhtsukaEmail author
  • Hiromi Miura
  • Hirofumi Nakaoka
  • Minoru Kimura
  • Masahiro Sato
  • Hidetoshi Inoko
Technical Update

Keywords

Epididymal Spermatozoon Germline Transmission Extra Sequence Donor Vector Fragment Cloning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Funded by the Grant-in-Aid for Young Scientists (B) (20700368) to M.O. from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

Supplementary material

11248_2011_9505_MOESM1_ESM.docx (151 kb)
Supplementary material 1 (DOCX 150 kb)

Reference

  1. Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H (2010) Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res 38:e198PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Masato Ohtsuka
    • 1
    Email author
  • Hiromi Miura
    • 1
  • Hirofumi Nakaoka
    • 2
  • Minoru Kimura
    • 1
  • Masahiro Sato
    • 3
  • Hidetoshi Inoko
    • 1
  1. 1.Department of Molecular Life Science, Division of Basic Medical Science and Molecular MedicineTokai University School of MedicineIseharaJapan
  2. 2.Division of Human Genetics, Department of Integrated GeneticsNational Institute of GeneticsMishimaJapan
  3. 3.Section of Gene Expression Regulation, Frontier Science Research CenterKagoshima UniversityKagoshimaJapan

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