Transgenic Research

, Volume 21, Issue 1, pp 225–226

Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs

  • Masato Ohtsuka
  • Hiromi Miura
  • Hirofumi Nakaoka
  • Minoru Kimura
  • Masahiro Sato
  • Hidetoshi Inoko
Technical Update

Supplementary material

11248_2011_9505_MOESM1_ESM.docx (151 kb)
Supplementary material 1 (DOCX 150 kb)

Reference

  1. Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H (2010) Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res 38:e198PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Masato Ohtsuka
    • 1
  • Hiromi Miura
    • 1
  • Hirofumi Nakaoka
    • 2
  • Minoru Kimura
    • 1
  • Masahiro Sato
    • 3
  • Hidetoshi Inoko
    • 1
  1. 1.Department of Molecular Life Science, Division of Basic Medical Science and Molecular MedicineTokai University School of MedicineIseharaJapan
  2. 2.Division of Human Genetics, Department of Integrated GeneticsNational Institute of GeneticsMishimaJapan
  3. 3.Section of Gene Expression Regulation, Frontier Science Research CenterKagoshima UniversityKagoshimaJapan

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