Transgenic Research

, Volume 20, Issue 6, pp 1367–1377 | Cite as

Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference

Original Paper


Rice stripe disease, with the pathogen Rice stripe virus (RSV), is one of the most widespread and severe virus diseases. Cultivating a resistant breed is an essential and efficient method in preventing rice stripe disease. Following RNA interference (RNAi) theory, we constructed three RNAi binary vectors based on coat protein (CP), special-disease protein (SP) and chimeric CP/SP gene sequence. Transgenic lines of rice cv. Yujing6 were generated through Agrobacterium-mediated transformation. We inoculated T1 generation plants from each line derived from CP/SP, CP, and SP transgenic rice plants with two RSV isolates from Shandong Province and Jiangsu Province using viruliferous vector insects. In these resistance assays, chimeric CP/SP RNAi lines showed stronger resistance against two isolates than CP or SP single RNAi lines. Stable integration and expression of RNAi transgenes were confirmed by Southern and northern blot analysis of independent transgenic lines. In the resistant transgenic lines, lower levels of transgene transcripts and specific short interference RNAs were observed relative to the susceptible transgenic plant, which showed that virus resistance was increased by RNAi. Genetic analysis demonstrated that transgene and virus resistance was stably inherited in the T2 progeny plants.


Rice stripe virus CP gene SP gene RNA interference Transgenic rice 



Bialaphos resistance gene


Coat protein


Double-strand RNA


Hairpin RNA




Open reading frame




Polymerase chain reaction


Posttranscriptional gene silencing


RNA-mediated virus resistance


RNA interference


Short interfering RNA


Special-disease protein


Viral RNA


Viral complementary RNA

Supplementary material

11248_2011_9502_MOESM1_ESM.pdf (8.9 mb)
Supplementary material 1 (PDF 9069 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anPeople’s Republic of China
  2. 2.Rice Science Research InstituteShandong Academy of Agricultural ScienceJiningPeople’s Republic of China

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