Transgenic Research

, Volume 20, Issue 6, pp 1285–1292

Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

  • Tadayoshi Hirai
  • You-Wang Kim
  • Kazuhisa Kato
  • Kyoko Hiwasa-Tanase
  • Hiroshi Ezura
Original Paper

DOI: 10.1007/s11248-011-9495-9

Cite this article as:
Hirai, T., Kim, YW., Kato, K. et al. Transgenic Res (2011) 20: 1285. doi:10.1007/s11248-011-9495-9

Abstract

The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.

Keywords

E8 promoter Miraculin Taste-modifying protein Tomato 

Supplementary material

11248_2011_9495_MOESM1_ESM.pdf (3.9 mb)
Supplementary material 1 (PDF 4027 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Tadayoshi Hirai
    • 1
  • You-Wang Kim
    • 1
  • Kazuhisa Kato
    • 1
  • Kyoko Hiwasa-Tanase
    • 1
  • Hiroshi Ezura
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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