Transgenic Research

, Volume 20, Issue 6, pp 1285–1292 | Cite as

Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

  • Tadayoshi Hirai
  • You-Wang Kim
  • Kazuhisa Kato
  • Kyoko Hiwasa-Tanase
  • Hiroshi EzuraEmail author
Original Paper


The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.


E8 promoter Miraculin Taste-modifying protein Tomato 



We thank the members of the Ezura laboratory for helpful discussions. “Micro-Tom” seeds (TOMJPF00001) were obtained from the National BioResource Project Tomato (NBRP-tomato). This research was supported by the project “Development of Fundamental Technologies for the Production of High-value Materials Using Transgenic Plants” from the Ministry of Economy, Trade, and Industry of Japan (to H.E.).

Supplementary material

11248_2011_9495_MOESM1_ESM.pdf (3.9 mb)
Supplementary material 1 (PDF 4027 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Tadayoshi Hirai
    • 1
  • You-Wang Kim
    • 1
  • Kazuhisa Kato
    • 1
  • Kyoko Hiwasa-Tanase
    • 1
  • Hiroshi Ezura
    • 1
    Email author
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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