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Transgenic Research

, Volume 20, Issue 3, pp 699–700 | Cite as

Erratum to: Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice

  • Pei-wen Pan
  • Katri Käyrä
  • Jukka Leinonen
  • Marja Nissinen
  • Seppo Parkkila
  • Hannu Rajaniemi
Erratum
First Online:

Erratum to: Transgenic Res DOI 10.1007/s11248-010-9441-2

Due to an update of the Car6 gene in GenBank®, the number of exons in Fig. 1A of the above mentioned publication have been changed from 7 to 8. Furthermore, the text describing the construction of Car6 targeting vector should be corrected accordingly. The correct representation of Fig. 1 and the concerning paragraph are published on the following page and should be treated as definitive by the reader.
Fig. 1

Targeted disruption of the Car6 gene in ES cells. A A schematic representation of the disruption of the Car6 locus by homologous recombination. Solid boxes represent exons. F1, R1, and R2 are primers used for genotyping. B Identification of targeted (±) ES cell clones using multiplex PCR. M molecular marker. C− indicates the negative control for PCR (H2O). C Characterization of targeted (±) ES cell clones by Southern blot

Within the section ‘Construction of Car6 targeting vector’ of the ‘Materials and methods’, the second sentence in the first paragraph should read:

“The vector (pPN2ThGHterm-Car6 targeting vector) was designed such that exons 3 and 4 of the mouse Car6 gene were replaced by the bacterial neomycin resistance gene (Neo).”

The first sentence in the second paragraph must read: “To construct the pPN2ThGHterm-Car6 targeting vector, a short 5′-homologous arm (2,058 bp) containing exon 2 was amplified using the entire mouse Car6 gene clone (described above) as a template.”

The fourth sentence in the second paragraph should read: “Subsequently, a long 3′-homologous arm (4,984 bp) containing exons 5 and 6 was amplified using the entire mouse Car6 gene clone as a template.”

The correct Fig. 1 and its caption are shown on the following page.

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Pei-wen Pan
    • 1
    • 3
  • Katri Käyrä
    • 4
  • Jukka Leinonen
    • 4
  • Marja Nissinen
    • 4
  • Seppo Parkkila
    • 1
    • 3
    • 2
  • Hannu Rajaniemi
    • 4
  1. 1.Institute of Medical TechnologyUniversity of TampereTampereFinland
  2. 2.School of MedicineUniversity of TampereTampereFinland
  3. 3.Centre for Laboratory Medicine, Tampere University HospitalUniversity of TampereTampereFinland
  4. 4.Department of Anatomy and Cell Biology, Institute of BiomedicineUniversity of OuluOuluFinland

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