GADD34 suppresses wound healing by upregulating expression of myosin IIA
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Wound healing consists of sequential steps of tissue repair, and cell migration is particularly important. In order to analyze the potential function of growth arrest and DNA damage inducible protein 34 (GADD34) in tissue repair, we performed in vitro and in vivo wound healing experiments. In an in vitro scratch assay, GADD34 knockout (KO) mouse embryonic fibroblasts (MEFs) had higher migration rates than did wild type (WT) MEFs. Furthermore, the rate of wound closure was faster in GADD34 KO MEFs than in WT MEFs. Using in vivo punch biopsy assays, GADD34 KO mice had accelerated wound healing compared to WT mice. WT mice expressed higher amounts of myosin IIA in migrating macrophages and myofibroblasts than did GADD34 KO mice. These results indicate that GADD34 negatively regulates cell migration in wound healing via expression of myosin IIA.
KeywordsGADD34 Wound healing Cell migration Myosin
We thank M. Tanaka for technical assistance with confocal imaging. This work was supported by the Ministry of Education, Science, Technology, Sports and Culture, Japan
- He B, Gross M, Roizman B (1998) The gamma(1)34.5 protein of herpes simplex virus 1 has the structural and functional attributes of a protein phosphatase I regulatory subunit and is present in a high molecular weight complex with the enzyme in infected cells. Journal of Biological Chemistry 273:20737–20743CrossRefPubMedGoogle Scholar