Gene expression profiles of MON810 and comparable non-GM maize varieties cultured in the field are more similar than are those of conventional lines
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Maize is a major food crop and genetically modified (GM) varieties represented 24% of the global production in 2007. Authorized GM organisms have been tested for human and environmental safety. We previously used microarrays to compare the transcriptome profiles of widely used commercial MON810 versus near-isogenic varieties and reported differential expression of a small set of sequences in leaves of in vitro cultured plants of AristisBt/Aristis and PR33P67/PR33P66 (Coll et al. 2008). Here we further assessed the significance of these differential expression patterns in plants grown in a real context, i.e. in the field. Most sequences that were differentially expressed in plants cultured in vitro had the same expression values in MON810 and comparable varieties when grown in the field; and no sequence was found to be differentially regulated in the two variety pairs grown in the field. The differential expression patterns observed between in vitro and field culture were similar between MON810 and comparable varieties, with higher divergence between the two conventional varieties. This further indicates that MON810 and comparable non-GM varieties are equivalent except for the introduced character.
KeywordsGMO (Genetically Modified Organism) MON810 Maize Field Unintended effects Expression profile
Certified reference material.
European Food Safety Authority.
Genetically modified organism.
Institute for Reference Materials and Measurements.
International Service for the Acquisition of Agri-biotech Applications.
- Real-time RT-PCR
Reverse transcription coupled to real-time polymerase chain reaction.
Vegetative two-leaf stage.
We specially thank T. Esteve (CRAG), J. Serra and J. Salvia (E. E. A. Mas Badia) for valuable suggestions. This work was financially supported by the Spanish MEC project with ref. AGL2007-65903/AGR. AC received a studentship from the Generalitat de Catalunya (2005FI 00144).
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