Expression of Dm-AMP1 in rice confers resistance to Magnaporthe oryzae and Rhizoctonia solani
Magnaporthe oryzae and Rhizoctonia solani, are among the most important pathogens of rice, severely limiting its productivity. Dm-AMP1, an antifungal plant defensin from Dahlia merckii, was expressed in rice (Oryza sativa L. sp. indica cv. Pusa basmati 1) using Agrobacterium tumefaciens-mediated transformation. Expression levels of Dm-AMP1 ranged from 0.43% to 0.57% of total soluble protein in transgenic plants. It was observed that constitutive expression of Dm-AMP1 suppresses the growth of M. oryzae and R. solani by 84% and 72%, respectively. Transgenic expression of Dm-AMP1 was not accompanied by an induction of pathogenesis-related (PR) gene expression, indicating that the expression of DmAMP1 directly inhibits the pathogen. The results of in vitro, in planta and microscopic analyses suggest that Dm-AMP1 expression has the potential to provide broad-spectrum disease resistance in rice.
KeywordsDmAMP1 Magnaporthe oryzae Rhizoctonia solani Transgenic rice Plant-microbe interactions Defensins
We thank Dr. B. Cammue, University of Leuven, Belgium, for providing the plasmid pFAJ3105. We also extend our thanks to Prof. R. N. Pandey, Anand Agricultural University, Anand, India, for providing cultures of R. solani. This work was supported by the grants from Department of Biotechnology, Ministry of Science and Technology, Government of India.
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