Transgenic Research

, Volume 17, Issue 5, pp 991–992 | Cite as

Immunohistochemical staining for tilapia and human insulin demonstrates that a tilapia transgenic for humanized insulin is a mosaic

  • James R. WrightJr
  • Jaime Snowdon
  • Olga Hrytsenko
  • Carol M. Morrison
  • Bill Pohajdak
Technical Update
First Online:
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Accepted:

Keywords

Site Directed Mutagenesis Human Insulin Teleost Fish Tight Cluster Immunoperoxidase Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgement

Funded by the Juvenile Diabetes Research Foundation.

References

  1. Pohajdak B, Mansour M, Hrytsenko O, Conlon JM, Dymond LC, Wright JR Jr (2004) Production of transgenic tilapia with Brockman bodies secreting [desThrB30] human insulin. Transgenic Res 13:313–323PubMedCrossRefGoogle Scholar
  2. Snowdon J (2003) Generation of a mouse monoclonal antibody against tilapia (Oreochromis niloticus) insulin: Application to histology and enzyme-linked immunosorbent assay. M.Sc. thesis, Dalhousie University, Halifax, NSGoogle Scholar
  3. Yang H, Morrison CM, Conlon JM, Laybolt K, Wright JR Jr (1999) Immunocytochemical characterization of the pancreatic islet cells of the tilapia (Oreochromis niloticus). Gen Comp Endocrinol 114:47–56PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • James R. WrightJr
    • 1
  • Jaime Snowdon
    • 2
  • Olga Hrytsenko
    • 3
  • Carol M. Morrison
    • 4
  • Bill Pohajdak
    • 3
  1. 1.Department of Pathology & Laboratory Medicine (Calgary Laboratory Services) and the Julia McFarlane Diabetes Research Centre, Faculty of MedicineUniversity of CalgaryCalgaryCanada
  2. 2.Department of Pathology, Faculty of MedicineDalhousie UniversityHalifaxCanada
  3. 3.Department of BiologyDalhousie UniversityHalifaxCanada
  4. 4.IWK Health CentreHalifaxCanada

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