Transgenic Research

, 17:651

Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica)

Original Paper

DOI: 10.1007/s11248-007-9145-4

Cite this article as:
Lal, S., Gulyani, V. & Khurana, P. Transgenic Res (2008) 17: 651. doi:10.1007/s11248-007-9145-4

Abstract

Late embryogenesis abundant (LEA) proteins are members of a large group of hydrophilic proteins found primarily in plants. The barley hva1 gene encodes a group 3 LEA protein and is induced by ABA and water deficit conditions. We report here the over expression of hva1 in mulberry under a constitutive promoter via Agrobacterium-mediated transformation. Molecular analysis of the transgenic plants revealed the stable integration and expression of the transgene in the transformants. Transgenic plants were subjected to simulated salinity and drought stress conditions to study the role of hva1 in conferring tolerance. The transgenic plants showed better cellular membrane stability (CMS), photosynthetic yield, less photo-oxidative damage and better water use efficiency as compared to the non-transgenic plants under both salinity and drought stress. Under salinity stress, transgenic plants show many fold increase in proline concentration than the non-transgenic plants and under water deficit conditions proline is accumulated only in the non-transgenic plants. Results also indicate that the production of HVA1 proteins helps in better performance of transgenic mulberry by protecting membrane stability of plasma membrane as well as chloroplastic membranes from injury under abiotic stress. Interestingly, it was observed that hva1 conferred different degrees of tolerance to the transgenic plants towards various stress conditions. Amongst the lines analysed for stress tolerance transgenic line ST8 was relatively more salt tolerant, ST30, ST31 more drought tolerant, and lines ST11 and ST6 responded well under both salinity and drought stress conditions as compared to the non-transgenic plants. Thus hva1 appears to confer a broad spectrum of tolerance under abiotic stress in mulberry.

Keywords

CMS Drought hva1 LEA Proline Mulberry Salinity Transgenic WUE 

Abbreviations

ABA

Abscisic acid

CMS

Cellular membrane stability

DW

Dry weight

FW

Fresh weight

IRMS

Isotope ratio mass spectrometry

LEA

Late embryogenesis abundant protein

MS

Murashige and Skoog

RBD

Randomized block design

RWC

Relative water content

TDZ

Thidiazuron

TW

Turgid weight

WUE

Water use efficiency

YEM

Yeast extract mannitol

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

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