Transgenic Research

, Volume 16, Issue 5, pp 661–664

Transgenic embryos and mice produced from low titre lentiviral vectors

  • William A. Ritchie
  • Clare Neil
  • Tim King
  • C. Bruce A. Whitelaw
Technical Report

Abstract

Lentiviral vectors are now recognised as an efficient transgene delivery system which can result in greater than 90% of founder animals carrying the transgene. Vector injection into the perivitelline space has emerged as the standard delivery method but is limited by the need for high-titre lentivirus vector preparations. Based on a modified perivitelline injection method we demonstrate that transgenic animals can be generated from low-titre virus vector preparations further simplifying lentiviral transgenesis. Repeat injection of 107 TU/ml vector preparation resulted in 23% of embryos carrying the transgene compare to 1% from a single injection. Embryos exposed to repeat injection of vector developed to blastocyst with the same efficiency as non-injected embryos and produced transgenic mice capable of transmitting the transgene through the germline

Keywords

Biotechnology Embryo Retrovirus Transgene 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • William A. Ritchie
    • 1
  • Clare Neil
    • 2
  • Tim King
    • 1
  • C. Bruce A. Whitelaw
    • 2
  1. 1.Animal Services DepartmentRoslin InstituteMidlothianUK
  2. 2.Department of Gene Function and DevelopmentRoslin InstituteMidlothianUK

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